This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_01F-N1-BDNA-1_B23KFG5LT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1560.992 CPU sec, 711.366 real sec
[M::process] read 676356 sequences (100000196 bp) in 0.861 CPU sec, 0.865 real sec...
[M::process] read 675198 sequences (100000016 bp) in 1.008 CPU sec, 1.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279950, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 374, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (385.84, 175.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674336 sequences (100000026 bp) in 1.036 CPU sec, 1.062 real sec...
[M::process] read 673690 sequences (100000288 bp) in 1.040 CPU sec, 1.037 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279540, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 387, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (397.04, 178.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676356 reads in 129.988 CPU sec, 4.141 real sec
[M::mem_process_seqs] Processed 675198 reads in 79.475 CPU sec, 2.536 real sec
sorting block 0, #reads = 1077873 mem = 536880467 peak = 602898432
[M::process] read 673348 sequences (100000203 bp) in 1.393 CPU sec, 1.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279483, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 403, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083)
[M::mem_pestat] mean and std.dev: (412.01, 184.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1355)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673450 sequences (100000197 bp) in 1.090 CPU sec, 1.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278769, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 411, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100)
[M::mem_pestat] mean and std.dev: (418.06, 185.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1376)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (344, 344, 1217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2963)
[M::mem_pestat] mean and std.dev: (831.36, 604.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3836)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278708, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 411, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090)
[M::mem_pestat] mean and std.dev: (418.54, 184.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1362)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674336 reads in 186.988 CPU sec, 6.016 real sec
[M::mem_process_seqs] Processed 673690 reads in 142.488 CPU sec, 4.618 real sec
[M::mem_process_seqs] Processed 673348 reads in 155.456 CPU sec, 5.051 real sec
sorting block 1, #reads = 1077313 mem = 536879127 peak = 731873280
[M::process] read 673300 sequences (100000240 bp) in 1.541 CPU sec, 1.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278400, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 404, 541)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085)
[M::mem_pestat] mean and std.dev: (413.52, 184.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1357)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 2, #reads = 1077052 mem = 536877565 peak = 790421504
[M::process] read 674654 sequences (100000184 bp) in 1.565 CPU sec, 1.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278595, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 407, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090)
[M::mem_pestat] mean and std.dev: (416.43, 185.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1363)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675274 sequences (100000179 bp) in 1.133 CPU sec, 1.154 real sec...
[M::mem_process_seqs] Processed 673450 reads in 147.073 CPU sec, 4.693 real sec
[M::mem_process_seqs] Processed 673300 reads in 92.608 CPU sec, 2.940 real sec
sorting block 3, #reads = 1075333 mem = 536877917 peak = 790421504
[M::process] read 674668 sequences (100000245 bp) in 1.600 CPU sec, 1.740 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279098, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 391, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (402.71, 182.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1317)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673888 sequences (100000171 bp) in 1.078 CPU sec, 1.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279295, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (393.83, 177.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674654 reads in 141.086 CPU sec, 4.517 real sec
[M::mem_process_seqs] Processed 675274 reads in 87.605 CPU sec, 2.812 real sec
sorting block 4, #reads = 1075445 mem = 536878711 peak = 790421504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279127, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 394, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (404.00, 181.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673256 sequences (100000172 bp) in 1.769 CPU sec, 1.895 real sec...
[M::process] read 673438 sequences (100000012 bp) in 1.160 CPU sec, 1.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279591, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 408, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080)
[M::mem_pestat] mean and std.dev: (415.83, 184.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1349)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674668 reads in 135.153 CPU sec, 4.337 real sec
[M::mem_process_seqs] Processed 673888 reads in 84.758 CPU sec, 2.702 real sec
sorting block 5, #reads = 1078316 mem = 536878033 peak = 790421504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 279541, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 414, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091)
[M::mem_pestat] mean and std.dev: (421.56, 185.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1362)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673768 sequences (100000146 bp) in 1.902 CPU sec, 2.020 real sec...
[M::process] read 673200 sequences (100000053 bp) in 1.190 CPU sec, 1.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279276, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 413, 551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105)
[M::mem_pestat] mean and std.dev: (420.99, 187.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1382)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673256 reads in 137.853 CPU sec, 4.416 real sec
sorting block 6, #reads = 1077882 mem = 536878214 peak = 790421504
[M::mem_process_seqs] Processed 673438 reads in 86.467 CPU sec, 2.763 real sec
sorting block 7, #reads = 1076038 mem = 536879928 peak = 878100480
[M::process] read 676206 sequences (100000175 bp) in 1.634 CPU sec, 1.760 real sec...
[M::process] read 675020 sequences (100000128 bp) in 1.490 CPU sec, 1.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278678, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 412, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096)
[M::mem_pestat] mean and std.dev: (420.21, 187.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1370)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673200 reads in 76.130 CPU sec, 2.414 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279077, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 406, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094)
[M::mem_pestat] mean and std.dev: (415.14, 186.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1369)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279125, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 373, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (383.75, 172.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674184 sequences (100000254 bp) in 1.296 CPU sec, 1.305 real sec...
[M::mem_process_seqs] Processed 673768 reads in 212.538 CPU sec, 6.796 real sec
sorting block 8, #reads = 1075088 mem = 536878300 peak = 878100480
[M::mem_process_seqs] Processed 676206 reads in 98.954 CPU sec, 3.185 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279896, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 381, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (390.77, 173.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1077336 mem = 536878129 peak = 878100480
[M::process] read 673730 sequences (100000204 bp) in 2.180 CPU sec, 2.400 real sec...
[M::process] read 673532 sequences (100000297 bp) in 1.240 CPU sec, 1.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279195, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 393, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (401.53, 175.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675020 reads in 139.857 CPU sec, 4.483 real sec
[M::mem_process_seqs] Processed 674184 reads in 87.807 CPU sec, 2.802 real sec
sorting block 10, #reads = 1078537 mem = 536877817 peak = 878100480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278605, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 404, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061)
[M::mem_pestat] mean and std.dev: (411.16, 180.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1324)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673832 sequences (100000222 bp) in 1.776 CPU sec, 1.909 real sec...
[M::process] read 673814 sequences (100000063 bp) in 1.260 CPU sec, 1.266 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278945, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 410, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094)
[M::mem_pestat] mean and std.dev: (417.52, 185.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1368)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673730 reads in 140.245 CPU sec, 4.495 real sec
[M::mem_process_seqs] Processed 673532 reads in 88.148 CPU sec, 2.819 real sec
sorting block 11, #reads = 1076533 mem = 536879739 peak = 878100480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278795, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 406, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103)
[M::mem_pestat] mean and std.dev: (414.47, 186.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1382)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674372 sequences (100000251 bp) in 2.181 CPU sec, 2.346 real sec...
[M::process] read 675848 sequences (100000152 bp) in 1.270 CPU sec, 1.279 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278881, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 407, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103)
[M::mem_pestat] mean and std.dev: (416.70, 187.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1381)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673832 reads in 142.738 CPU sec, 4.564 real sec
[M::mem_process_seqs] Processed 673814 reads in 87.787 CPU sec, 2.804 real sec
sorting block 12, #reads = 1076679 mem = 536880913 peak = 878100480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278546, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 394, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061)
[M::mem_pestat] mean and std.dev: (405.25, 183.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1327)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674872 sequences (100000094 bp) in 2.267 CPU sec, 2.397 real sec...
[M::process] read 674052 sequences (100000067 bp) in 1.270 CPU sec, 1.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279220, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 376, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (386.14, 173.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674372 reads in 141.038 CPU sec, 4.515 real sec
sorting block 13, #reads = 1075830 mem = 536879839 peak = 878100480
[M::mem_process_seqs] Processed 675848 reads in 88.377 CPU sec, 2.819 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278395, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 389, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (398.23, 176.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1077644 mem = 536880451 peak = 906768384
[M::process] read 674066 sequences (100000161 bp) in 2.223 CPU sec, 2.436 real sec...
[M::process] read 673372 sequences (100000128 bp) in 1.300 CPU sec, 1.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278026, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 408, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087)
[M::mem_pestat] mean and std.dev: (414.20, 183.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1359)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674872 reads in 144.418 CPU sec, 4.631 real sec
[M::mem_process_seqs] Processed 674052 reads in 90.817 CPU sec, 2.904 real sec
sorting block 15, #reads = 1076423 mem = 536877828 peak = 906768384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278300, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 409, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092)
[M::mem_pestat] mean and std.dev: (415.36, 184.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1366)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674132 sequences (100000273 bp) in 2.116 CPU sec, 2.250 real sec...
[M::process] read 673212 sequences (100000066 bp) in 1.290 CPU sec, 1.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278406, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 413, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093)
[M::mem_pestat] mean and std.dev: (418.16, 183.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1366)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674066 reads in 148.399 CPU sec, 4.753 real sec
[M::mem_process_seqs] Processed 673372 reads in 91.730 CPU sec, 2.930 real sec
sorting block 16, #reads = 1074835 mem = 536881016 peak = 906768384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278530, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 400, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087)
[M::mem_pestat] mean and std.dev: (409.85, 183.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1361)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681394 sequences (100000005 bp) in 2.219 CPU sec, 2.404 real sec...
[M::process] read 679560 sequences (100000097 bp) in 1.300 CPU sec, 1.295 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278846, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 410, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095)
[M::mem_pestat] mean and std.dev: (418.32, 185.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1369)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674132 reads in 141.311 CPU sec, 4.503 real sec
[M::mem_process_seqs] Processed 673212 reads in 87.688 CPU sec, 2.787 real sec
sorting block 17, #reads = 1075539 mem = 536879149 peak = 906768384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281502, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 330, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (356.54, 178.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677306 sequences (100000136 bp) in 2.293 CPU sec, 2.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280791, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 345, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (368.07, 180.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676740 sequences (100000097 bp) in 1.280 CPU sec, 1.275 real sec...
[M::mem_process_seqs] Processed 681394 reads in 140.533 CPU sec, 4.477 real sec
sorting block 18, #reads = 1075202 mem = 536882727 peak = 906768384
[M::mem_process_seqs] Processed 679560 reads in 87.557 CPU sec, 2.777 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280528, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 365, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (380.73, 179.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1081036 mem = 536879809 peak = 961679360
[M::process] read 675976 sequences (100000158 bp) in 2.299 CPU sec, 2.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279532, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 370, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (384.62, 179.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675720 sequences (100000197 bp) in 1.300 CPU sec, 1.306 real sec...
[M::mem_process_seqs] Processed 677306 reads in 138.783 CPU sec, 4.424 real sec
[M::mem_process_seqs] Processed 676740 reads in 87.685 CPU sec, 2.779 real sec
sorting block 20, #reads = 1079004 mem = 536878185 peak = 961679360
[M::process] read 677000 sequences (100000293 bp) in 2.277 CPU sec, 2.421 real sec...
[M::process] read 681600 sequences (100000032 bp) in 1.300 CPU sec, 1.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279972, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 376, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (389.24, 179.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1311)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675720 reads in 82.447 CPU sec, 2.621 real sec
sorting block 21, #reads = 1076553 mem = 536881688 peak = 961679360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279931, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 376, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (389.21, 179.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1314)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280105, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 364, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067)
[M::mem_pestat] mean and std.dev: (384.97, 185.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1344)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679846 sequences (100000234 bp) in 1.372 CPU sec, 1.374 real sec...
[M::mem_process_seqs] Processed 675976 reads in 219.486 CPU sec, 6.995 real sec
[M::mem_process_seqs] Processed 677000 reads in 102.780 CPU sec, 3.291 real sec
sorting block 22, #reads = 1076302 mem = 536876649 peak = 961679360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281864, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 329, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (355.37, 178.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677504 sequences (100000118 bp) in 2.257 CPU sec, 2.410 real sec...
[M::process] read 676834 sequences (100000037 bp) in 1.260 CPU sec, 1.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281191, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 344, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (367.86, 182.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681600 reads in 136.007 CPU sec, 4.332 real sec
[M::mem_process_seqs] Processed 679846 reads in 85.391 CPU sec, 2.711 real sec
sorting block 23, #reads = 1080004 mem = 536878580 peak = 961679360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279309, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 365, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (381.23, 181.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676050 sequences (100000266 bp) in 1.925 CPU sec, 2.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279595, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 372, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (387.00, 182.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675726 sequences (100000055 bp) in 1.250 CPU sec, 1.253 real sec...
[M::mem_process_seqs] Processed 677504 reads in 134.696 CPU sec, 4.303 real sec
[M::mem_process_seqs] Processed 676834 reads in 84.584 CPU sec, 2.683 real sec
sorting block 24, #reads = 1079873 mem = 536875968 peak = 961679360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279248, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 379, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (392.36, 182.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1331)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677488 sequences (100000235 bp) in 2.205 CPU sec, 2.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280151, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 383, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080)
[M::mem_pestat] mean and std.dev: (397.75, 186.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1357)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681400 sequences (100000223 bp) in 1.350 CPU sec, 1.342 real sec...
[M::mem_process_seqs] Processed 676050 reads in 134.505 CPU sec, 4.284 real sec
sorting block 25, #reads = 1078044 mem = 536880491 peak = 961679360
[M::mem_process_seqs] Processed 675726 reads in 85.953 CPU sec, 2.727 real sec
sorting block 26, #reads = 1077980 mem = 536879418 peak = 961679360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279950, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 358, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069)
[M::mem_pestat] mean and std.dev: (382.41, 187.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1348)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679488 sequences (100000127 bp) in 1.811 CPU sec, 1.933 real sec...
[M::process] read 677496 sequences (100000131 bp) in 1.280 CPU sec, 1.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281161, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 330, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (355.47, 177.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677488 reads in 141.651 CPU sec, 4.521 real sec
[M::mem_process_seqs] Processed 681400 reads in 86.530 CPU sec, 2.757 real sec
sorting block 27, #reads = 1078782 mem = 536879357 peak = 961679360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280520, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 347, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (368.94, 180.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677164 sequences (100000008 bp) in 2.195 CPU sec, 2.305 real sec...
[M::process] read 676922 sequences (100000180 bp) in 1.310 CPU sec, 1.306 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279999, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 364, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (378.82, 177.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679488 reads in 139.584 CPU sec, 4.448 real sec
[M::mem_process_seqs] Processed 677496 reads in 85.753 CPU sec, 2.725 real sec
sorting block 28, #reads = 1080644 mem = 536878596 peak = 961679360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280208, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 367, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (382.25, 179.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676248 sequences (100000124 bp) in 1.884 CPU sec, 2.016 real sec...
[M::process] read 679574 sequences (100000146 bp) in 1.300 CPU sec, 1.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279579, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 370, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056)
[M::mem_pestat] mean and std.dev: (385.58, 181.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1328)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677164 reads in 144.350 CPU sec, 4.614 real sec
[M::mem_process_seqs] Processed 676922 reads in 89.034 CPU sec, 2.834 real sec
sorting block 29, #reads = 1078910 mem = 536878816 peak = 961679360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279924, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 375, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076)
[M::mem_pestat] mean and std.dev: (390.81, 184.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1354)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681406 sequences (100000174 bp) in 2.174 CPU sec, 2.265 real sec...
[M::process] read 678918 sequences (100000283 bp) in 1.300 CPU sec, 1.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280610, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 341, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (368.65, 185.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676248 reads in 148.566 CPU sec, 4.736 real sec
sorting block 30, #reads = 1076534 mem = 536879303 peak = 961679360
[M::mem_process_seqs] Processed 679574 reads in 92.378 CPU sec, 2.941 real sec
sorting block 31, #reads = 1076206 mem = 536880330 peak = 972988416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281290, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 332, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (356.14, 176.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677446 sequences (100000031 bp) in 1.858 CPU sec, 1.981 real sec...
[M::process] read 677132 sequences (100000009 bp) in 1.260 CPU sec, 1.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279975, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 352, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (371.39, 179.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681406 reads in 143.859 CPU sec, 4.595 real sec
[M::mem_process_seqs] Processed 678918 reads in 88.205 CPU sec, 2.813 real sec
sorting block 32, #reads = 1081676 mem = 536877829 peak = 972988416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279189, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 362, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (377.82, 177.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675898 sequences (100000123 bp) in 2.148 CPU sec, 2.259 real sec...
[M::process] read 675816 sequences (100000231 bp) in 1.270 CPU sec, 1.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279296, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 362, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (377.94, 176.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677446 reads in 144.731 CPU sec, 4.620 real sec
[M::mem_process_seqs] Processed 677132 reads in 89.129 CPU sec, 2.841 real sec
sorting block 33, #reads = 1078876 mem = 536879777 peak = 972988416
[M::process] read 421032 sequences (62304074 bp) in 1.399 CPU sec, 1.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278944, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 375, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (387.41, 177.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278920, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 378, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (392.06, 181.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1327)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675898 reads in 142.509 CPU sec, 4.612 real sec
[M::mem_process_seqs] Processed 675816 reads in 87.871 CPU sec, 2.851 real sec
sorting block 34, #reads = 1078312 mem = 536882667 peak = 972988416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 173924, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 378, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (396.77, 188.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1384)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 421032 reads in 49.984 CPU sec, 1.673 real sec
sorting block 35, #reads = 1076387 mem = 536878108 peak = 1042079744
os version: Linux 6.12.83
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 38950752
overall: 69669945344 mem 5712.990 user 242.909 sys 859.805 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KFG5LT4.1029_01F-N1-BDNA-1_B23KFG5LT4_1.L008\tPU:L008\tSM:1029_01F-N1-BDNA-1_B23KFG5LT4_1\tLB:1029_01F-N1-BDNA-1_B23KFG5LT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1029_01F-N1-BDNA-1_B23KFG5LT4_1_R1.fastq.gz 1029_01F-N1-BDNA-1_B23KFG5LT4_1_R2.fastq.gz
[main] Real time: 859.840 sec; CPU: 5955.902 sec
sorting block 36, #reads = 343515 mem = 171359072 peak = 1042079744
uniq header count 1
merging 37 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.83
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 1935824494
output reads: 39133536
bam_mem_sort: 37 calls 16.574 user 0.266 sys 16.921 real
bam_write: 37 calls 9.185 user 0.297 sys 9.492 real
execute: 1 calls 7.513 user 0.530 sys 870.288 real
merge_files: 1 calls 7.505 user 0.498 sys 10.125 real
parse_chunk: 582 calls 22.215 user 5.703 sys 28.228 real
read_chunk: 1568 calls 10.775 user 13.163 sys 147.095 real
sort_block: 1 calls 9.776 user 12.641 sys 143.773 real
write_chunk: 984 calls 7.116 user 0.354 sys 9.411 real
overall: 2323951616 mem 397.889 user 23.937 sys 870.310 real
ls: cannot access '*.cram': No such file or directory