Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... No adapter detected for read2 Read1 before filtering: total reads: 20805724 total bases: 3141664324 Q20 bases: 3087351027(98.2712%) Q30 bases: 2970711194(94.5585%) Read2 before filtering: total reads: 20805724 total bases: 3141664324 Q20 bases: 3073176519(97.82%) Q30 bases: 2929422640(93.2443%) Read1 after filtering: total reads: 20518494 total bases: 2719630348 Q20 bases: 2703034494(99.3898%) Q30 bases: 2628497935(96.6491%) Read2 after filtering: total reads: 20518494 total bases: 2723012580 Q20 bases: 2700939117(99.1894%) Q30 bases: 2623938708(96.3616%) Filtering result: reads passed filter: 41036988 reads failed due to low quality: 456540 reads failed due to too many N: 4498 reads failed due to too short: 113422 reads with adapter trimmed: 22815794 bases trimmed due to adapters: 761096974 Duplication rate: 28.3176% Insert size peak (evaluated by paired-end reads): 140 JSON report: 659_beZ_T1_TRNA_2_B2372Y3LT4_1.fastp.json HTML report: 659_beZ_T1_TRNA_2_B2372Y3LT4_1.fastp.html fastp --in1 659_beZ_T1_TRNA_2_B2372Y3LT4_1_1.fastq.gz --in2 659_beZ_T1_TRNA_2_B2372Y3LT4_1_2.fastq.gz --out1 659_beZ_T1_TRNA_2_B2372Y3LT4_1_1.fastp.fastq.gz --out2 659_beZ_T1_TRNA_2_B2372Y3LT4_1_2.fastp.fastq.gz --json 659_beZ_T1_TRNA_2_B2372Y3LT4_1.fastp.json --html 659_beZ_T1_TRNA_2_B2372Y3LT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 86 seconds