Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... No adapter detected for read2 Read1 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44635834562(98.5339%) Q30 bases: 43086927968(95.1146%) Read2 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44512884541(98.2624%) Q30 bases: 42755967249(94.384%) Read1 after filtering: total reads: 297370270 total bases: 42124893750 Q20 bases: 41819916169(99.276%) Q30 bases: 40601900576(96.3846%) Read2 after filtering: total reads: 297370270 total bases: 42132809902 Q20 bases: 41778066750(99.158%) Q30 bases: 40603239429(96.3696%) Filtering result: reads passed filter: 594740540 reads failed due to low quality: 4402358 reads failed due to too many N: 853168 reads failed due to too short: 3934 reads with adapter trimmed: 147103081 bases trimmed due to adapters: 5568148991 Duplication rate: 21.1566% Insert size peak (evaluated by paired-end reads): 161 JSON report: dna_s731.fastp.json HTML report: dna_s731.fastp.html fastp --in1 dna_s731_1.fastq.gz --in2 dna_s731_2.fastq.gz --out1 dna_s731_1.fastp.fastq.gz --out2 dna_s731_2.fastp.fastq.gz --json dna_s731.fastp.json --html dna_s731.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 1030 seconds