Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... No adapter detected for read2 Read1 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44487156202(98.2056%) Q30 bases: 42781192625(94.4397%) Read2 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44426562552(98.0719%) Q30 bases: 42582867715(94.0019%) Read1 after filtering: total reads: 297671777 total bases: 40278591682 Q20 bases: 39976612122(99.2503%) Q30 bases: 38836033255(96.4185%) Read2 after filtering: total reads: 297671777 total bases: 40290480424 Q20 bases: 39983877655(99.239%) Q30 bases: 38986781395(96.7643%) Filtering result: reads passed filter: 595343554 reads failed due to low quality: 4030548 reads failed due to too many N: 623064 reads failed due to too short: 2834 reads with adapter trimmed: 239614797 bases trimmed due to adapters: 9361326793 Duplication rate: 19.3116% Insert size peak (evaluated by paired-end reads): 120 JSON report: dna_s736.fastp.json HTML report: dna_s736.fastp.html fastp --in1 dna_s736_1.fastq.gz --in2 dna_s736_2.fastq.gz --out1 dna_s736_1.fastp.fastq.gz --out2 dna_s736_2.fastp.fastq.gz --json dna_s736.fastp.json --html dna_s736.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 1018 seconds