Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... No adapter detected for read2 Read1 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44247614758(97.6769%) Q30 bases: 42456157090(93.7222%) Read2 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44244651662(97.6703%) Q30 bases: 42232038274(93.2275%) Read1 after filtering: total reads: 297769585 total bases: 39146942413 Q20 bases: 38841419042(99.2195%) Q30 bases: 37696437553(96.2947%) Read2 after filtering: total reads: 297769585 total bases: 39160555615 Q20 bases: 38876503859(99.2746%) Q30 bases: 37955730607(96.9234%) Filtering result: reads passed filter: 595539170 reads failed due to low quality: 3812126 reads failed due to too many N: 644314 reads failed due to too short: 4390 reads with adapter trimmed: 287892695 bases trimmed due to adapters: 11658864733 Duplication rate: 21.1509% Insert size peak (evaluated by paired-end reads): 111 JSON report: dna_s745.fastp.json HTML report: dna_s745.fastp.html fastp --in1 dna_s745_1.fastq.gz --in2 dna_s745_2.fastq.gz --out1 dna_s745_1.fastp.fastq.gz --out2 dna_s745_2.fastp.fastq.gz --json dna_s745.fastp.json --html dna_s745.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 1031 seconds