Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... No adapter detected for read2 Read1 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44675549869(98.6215%) Q30 bases: 43098883769(95.141%) Read2 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44512918198(98.2625%) Q30 bases: 42739114546(94.3468%) Read1 after filtering: total reads: 297453087 total bases: 41474372522 Q20 bases: 41176422834(99.2816%) Q30 bases: 40018284723(96.4892%) Read2 after filtering: total reads: 297453087 total bases: 41484208588 Q20 bases: 41148697221(99.1912%) Q30 bases: 40039280103(96.5169%) Filtering result: reads passed filter: 594906174 reads failed due to low quality: 4374874 reads failed due to too many N: 715058 reads failed due to too short: 3894 reads with adapter trimmed: 180775223 bases trimmed due to adapters: 6899218919 Duplication rate: 19.0185% Insert size peak (evaluated by paired-end reads): 151 JSON report: dna_s740.fastp.json HTML report: dna_s740.fastp.html fastp --in1 dna_s740_1.fastq.gz --in2 dna_s740_2.fastq.gz --out1 dna_s740_1.fastp.fastq.gz --out2 dna_s740_2.fastp.fastq.gz --json dna_s740.fastp.json --html dna_s740.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 998 seconds