Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5f/feb53137fcee7a650a946cf300cf88/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-inputs/illumina_run_folders/data/20251009_LH00166_0186_B2372Y3LT4/RunParameters.xml Downloading: s3://natera-platform-sandbox/pipeline-inputs/illumina_run_folders/data/20251009_LH00166_0186_B2372Y3LT4/bclconvert_samplesheet_newRNAids.csv Downloading: s3://natera-platform-sandbox/pipeline-inputs/illumina_run_folders/data/20251009_LH00166_0186_B2372Y3LT4/Data/Intensities/s.locs Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5f/feb53137fcee7a650a946cf300cf88/.command.run Downloading: s3://natera-platform-sandbox/pipeline-inputs/illumina_run_folders/data/20251009_LH00166_0186_B2372Y3LT4/RunInfo.xml Downloading: s3://natera-platform-sandbox/pipeline-inputs/illumina_run_folders/data/20251009_LH00166_0186_B2372Y3LT4/Data/Intensities/BaseCalls/L004 ==> STAGING COMPLETE (7 inputs) WARNING: skipping lane 1 because RunInfo.xml file lists no tiles for it WARNING: skipping lane 2 because RunInfo.xml file lists no tiles for it WARNING: skipping lane 3 because RunInfo.xml file lists no tiles for it WARNING: skipping lane 5 because RunInfo.xml file lists no tiles for it WARNING: skipping lane 6 because RunInfo.xml file lists no tiles for it WARNING: skipping lane 7 because RunInfo.xml file lists no tiles for it WARNING: skipping lane 8 because RunInfo.xml file lists no tiles for it Sample sheet being processed by common lib? Yes SampleSheet Settings: FastqCompressionFormat = gzip NoLaneSplitting = FALSE OverrideCycles = Y151;I8;I8;Y151 SoftwareVersion = 4.3.6 Index Read 2 (i5): The barcode and UMI outputs will be processed and output in Reverse Complement of Sample Sheet inputs. OverrideCycles bases for this read will be interpreted in reverse. shared-thread-linux-native-asio output is disabled bcl-convert Version 4.4.6 Copyright (c) 2014-2025 Illumina, Inc. Command Line: --tiles s_4 --output-directory output --bcl-input-directory rundir --sample-sheet rundir/SampleSheet.csv Conversion Begins. Input BCL Information: Read Structure: Y151;I8;I8;Y151 RunInfo.xml file lists: 8 lanes, 6272 tiles BCL file format: cbcl Positions file format: slocs Filter file format: filter Conversion Information: Tile filtering: converting 784/6272 tiles Sample Count for Lane 1: 48 Lane 1 will be skipped because there are no tiles to convert (not listed in RunInfo.xml tile list?) Sample Count for Lane 2: 48 Lane 2 will be skipped because there are no tiles to convert (not listed in RunInfo.xml tile list?) Sample Count for Lane 3: 48 Lane 3 will be skipped because there are no tiles to convert (not listed in RunInfo.xml tile list?) Sample Count for Lane 4: 48 Sample Count for Lane 5: 24 Lane 5 will be skipped because there are no tiles to convert (not listed in RunInfo.xml tile list?) Sample Count for Lane 6: 24 Lane 6 will be skipped because there are no tiles to convert (not listed in RunInfo.xml tile list?) Sample Count for Lane 7: 24 Lane 7 will be skipped because there are no tiles to convert (not listed in RunInfo.xml tile list?) Sample Count for Lane 8: 24 Lane 8 will be skipped because there are no tiles to convert (not listed in RunInfo.xml tile list?) Expected number of sample FASTQ files to be created: 96 # CPU hw threads available: 64 Parallel Tiles: 4. Threads Per Tile: 16 SW compressors: 64 SW decompressors: 32 SW FASTQ compression level: 1 Lane 4 Post-run Information: Total clusters: 4176716290 Total Genomic Reads: 8353432580 Total Genomic Input Gigabases 1261.37 Minimum reads for a sample: 35210 Maximum reads for a sample: 419696556 Average reads per sample: 164842075.92 Undetermined reads: 441012936 Conversion Complete.