Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... No adapter detected for read2 Read1 before filtering: total reads: 127559649 total bases: 19261506999 Q20 bases: 18984411367(98.5614%) Q30 bases: 18330827550(95.1682%) Read2 before filtering: total reads: 127559649 total bases: 19261506999 Q20 bases: 18944047385(98.3518%) Q30 bases: 18218097711(94.5829%) Read1 after filtering: total reads: 126338015 total bases: 17974569763 Q20 bases: 17845041123(99.2794%) Q30 bases: 17329261691(96.4099%) Read2 after filtering: total reads: 126338015 total bases: 17977745281 Q20 bases: 17826790521(99.1603%) Q30 bases: 17321795474(96.3513%) Filtering result: reads passed filter: 252676030 reads failed due to low quality: 2087576 reads failed due to too many N: 354434 reads failed due to too short: 1258 reads with adapter trimmed: 59622879 bases trimmed due to adapters: 2210920324 Duplication rate: 12.9848% Insert size peak (evaluated by paired-end reads): 179 JSON report: dna_s764.fastp.json HTML report: dna_s764.fastp.html fastp --in1 dna_s764_1.fastq.gz --in2 dna_s764_2.fastq.gz --out1 dna_s764_1.fastp.fastq.gz --out2 dna_s764_2.fastp.fastq.gz --json dna_s764.fastp.json --html dna_s764.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 311 seconds