[W::hts_idx_load3] The index file is older than the data file: dna_s758.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15052) splicing reads. 4295 splicing junctions. 1247 known splicing junctions. 3048 novel splicing junctions.
sampling 10% (30105) splicing reads. 6525 splicing junctions. 1462 known splicing junctions. 5063 novel splicing junctions.
sampling 15% (45158) splicing reads. 8242 splicing junctions. 1599 known splicing junctions. 6643 novel splicing junctions.
sampling 20% (60211) splicing reads. 9706 splicing junctions. 1686 known splicing junctions. 8020 novel splicing junctions.
sampling 25% (75264) splicing reads. 11017 splicing junctions. 1744 known splicing junctions. 9273 novel splicing junctions.
sampling 30% (90317) splicing reads. 12160 splicing junctions. 1811 known splicing junctions. 10349 novel splicing junctions.
sampling 35% (105370) splicing reads. 13179 splicing junctions. 1846 known splicing junctions. 11333 novel splicing junctions.
sampling 40% (120423) splicing reads. 14154 splicing junctions. 1892 known splicing junctions. 12262 novel splicing junctions.
sampling 45% (135476) splicing reads. 15031 splicing junctions. 1917 known splicing junctions. 13114 novel splicing junctions.
sampling 50% (150529) splicing reads. 15768 splicing junctions. 1944 known splicing junctions. 13824 novel splicing junctions.
sampling 55% (165582) splicing reads. 16505 splicing junctions. 1961 known splicing junctions. 14544 novel splicing junctions.
sampling 60% (180635) splicing reads. 17197 splicing junctions. 1982 known splicing junctions. 15215 novel splicing junctions.
sampling 65% (195688) splicing reads. 17823 splicing junctions. 1993 known splicing junctions. 15830 novel splicing junctions.
sampling 70% (210741) splicing reads. 18409 splicing junctions. 2006 known splicing junctions. 16403 novel splicing junctions.
sampling 75% (225794) splicing reads. 18906 splicing junctions. 2016 known splicing junctions. 16890 novel splicing junctions.
sampling 80% (240847) splicing reads. 19399 splicing junctions. 2022 known splicing junctions. 17377 novel splicing junctions.
sampling 85% (255900) splicing reads. 19862 splicing junctions. 2025 known splicing junctions. 17837 novel splicing junctions.
sampling 90% (270953) splicing reads. 20278 splicing junctions. 2029 known splicing junctions. 18249 novel splicing junctions.
sampling 95% (286006) splicing reads. 20660 splicing junctions. 2035 known splicing junctions. 18625 novel splicing junctions.
sampling 100% (301059) splicing reads. 20986 splicing junctions. 2037 known splicing junctions. 18949 novel splicing junctions.