Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ed/283a0033be478e7ebee82df5a11747/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ed/283a0033be478e7ebee82df5a11747/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9a/83d63ee43dab72fc07dabf2a4e8ce9/output/HCC1395_BL_S2_L007_R2_001.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9a/83d63ee43dab72fc07dabf2a4e8ce9/output/HCC1395_BL_S2_L007_R1_001.fastq.gz ==> STAGING COMPLETE (4 inputs) Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 62452 total bases: 9367800 Q20 bases: 9203672(98.248%) Q30 bases: 8884176(94.8374%) Q40 bases: 8884176(94.8374%) Read2 before filtering: total reads: 62452 total bases: 9367800 Q20 bases: 9144281(97.614%) Q30 bases: 8751992(93.4263%) Q40 bases: 8751992(93.4263%) Read1 after filtering: total reads: 62452 total bases: 9015423 Q20 bases: 8886185(98.5665%) Q30 bases: 8596762(95.3562%) Q40 bases: 8596762(95.3562%) Read2 after filtering: total reads: 62452 total bases: 9015944 Q20 bases: 8824465(97.8762%) Q30 bases: 8468391(93.9268%) Q40 bases: 8468391(93.9268%) Filtering result: reads passed filter: 124904 reads failed due to low quality: 0 reads failed due to too many N: 0 reads failed due to too short: 0 reads with adapter trimmed: 19335 bases trimmed due to adapters: 704233 Duplication rate: 0.922308% Insert size peak (evaluated by paired-end reads): 191 JSON report: HCC1395_BL.fastp.json HTML report: HCC1395_BL.fastp.html fastp --in1 HCC1395_BL_S2_L007_R1_001.fastq.gz --in2 HCC1395_BL_S2_L007_R2_001.fastq.gz --out1 HCC1395_BL/HCC1395_BL_R1.fastq.gz --out2 HCC1395_BL/HCC1395_BL_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json HCC1395_BL.fastp.json --html HCC1395_BL.fastp.html --thread 4 fastp v1.0.1, time used: 2 seconds