Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c1/9abc49c711512d0d431ba088ffdec1/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c1/9abc49c711512d0d431ba088ffdec1/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/04/fed074c5ef2ce5273e428877ea48a0/output/Sig_18_tissue_S3_L001_R1_001.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/04/fed074c5ef2ce5273e428877ea48a0/output/Sig_18_tissue_S3_L001_R2_001.fastq.gz
==> STAGING COMPLETE (4 inputs)

Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

Read1 before filtering:
total reads: 62577
total bases: 9386550
Q20 bases: 9225636(98.2857%)
Q30 bases: 8949673(95.3457%)
Q40 bases: 8949673(95.3457%)

Read2 before filtering:
total reads: 62577
total bases: 9386550
Q20 bases: 9171645(97.7105%)
Q30 bases: 8888189(94.6907%)
Q40 bases: 8888189(94.6907%)

Read1 after filtering:
total reads: 62577
total bases: 8854472
Q20 bases: 8751144(98.833%)
Q30 bases: 8514296(96.1581%)
Q40 bases: 8514296(96.1581%)

Read2 after filtering:
total reads: 62577
total bases: 8854952
Q20 bases: 8695982(98.2047%)
Q30 bases: 8464643(95.5922%)
Q40 bases: 8464643(95.5922%)

Filtering result:
reads passed filter: 125154
reads failed due to low quality: 0
reads failed due to too many N: 0
reads failed due to too short: 0
reads with adapter trimmed: 29557
bases trimmed due to adapters: 1063676

Duplication rate: 0.754271%

Insert size peak (evaluated by paired-end reads): 170

JSON report: Sig_18_tissue.fastp.json
HTML report: Sig_18_tissue.fastp.html

fastp --in1 Sig_18_tissue_S3_L001_R1_001.fastq.gz --in2 Sig_18_tissue_S3_L001_R2_001.fastq.gz --out1 Sig_18_tissue/Sig_18_tissue_R1.fastq.gz --out2 Sig_18_tissue/Sig_18_tissue_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json Sig_18_tissue.fastp.json --html Sig_18_tissue.fastp.html --thread 4 
fastp v1.0.1, time used: 2 seconds