fastp report

Summary

General

fastp version:	1.0.1 (https://github.com/OpenGene/fastp)
sequencing:	paired end (150 cycles + 150 cycles)
mean length before filtering:	150bp, 150bp
mean length after filtering:	141bp, 141bp
duplication rate:	0.754271%
Insert size peak:	170
Detected read1 adapter:	AGATCGGAAGAGCACACGTCTGAACTCCAGTCA ->Illumina TruSeq Adapter Read 1
Detected read2 adapter:	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT ->Illumina TruSeq Adapter Read 2

Before filtering

total reads:	125.154000 K
total bases:	18.773100 M
Q20 bases:	18.397281 M (97.998098%)
Q30 bases:	17.837862 M (95.018202%)
Q40 bases:	17.837862 M (95.018202%)
GC content:	50.229760%

After filtering

total reads:	125.154000 K
total bases:	17.709424 M
Q20 bases:	17.447126 M (98.518879%)
Q30 bases:	16.978939 M (95.875162%)
Q40 bases:	16.978939 M (95.875162%)
GC content:	50.130925%

Filtering result

reads passed filters:	125.154000 K (100.000000%)
reads with low quality:	0 (0.000000%)
reads with too many N:	0 (0.000000%)
reads too short:	0 (0.000000%)

Adapters

Adapter or bad ligation of read1 Sequence Occurrences A 210 AG 238 AGA 218 AGAT 250 AGATC 262 AGATCG 217 AGATCGG 235 AGATCGGA 218 AGATCGGAA 228 AGATCGGAAG 216 AGATCGGAAGA 209 AGATCGGAAGAG 215 AGATCGGAAGAGC 229 AGATCGGAAGAGCA 238 AGATCGGAAGAGCAC 228 AGATCGGAAGAGCACA 203 AGATCGGAAGAGCACAC 227 AGATCGGAAGAGCACACG 215 AGATCGGAAGAGCACACGT 180 AGATCGGAAGAGCACACGTC 229 AGATCGGAAGAGCACACGTCT 243 AGATCGGAAGAGCACACGTCTG 187 AGATCGGAAGAGCACACGTCTGA 228 AGATCGGAAGAGCACACGTCTGAA 205 AGATCGGAAGAGCACACGTCTGAAC 191 AGATCGGAAGAGCACACGTCTGAACT 214 AGATCGGAAGAGCACACGTCTGAACTC 201 AGATCGGAAGAGCACACGTCTGAACTCC 215 AGATCGGAAGAGCACACGTCTGAACTCCA 222 AGATCGGAAGAGCACACGTCTGAACTCCAG 196 AGATCGGAAGAGCACACGTCTGAACTCCAGT 205 AGATCGGAAGAGCACACGTCTGAACTCCAGTC 230 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA 207 AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC 178 AGATCGGAAGAGCACACGTCTGAACTCCAGTCACA 196 AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC 177 other adapter sequences 7033

Adapter or bad ligation of read2 Sequence Occurrences A 211 AA 241 AGA 202 AGAA 250 AGATA 194 AGATCA 193 AGATCGA 215 AGATCGGA 201 AGATCGGAA 230 AGATCGGAAA 217 AGATCGGAAGA 186 AGATCGGAAGAA 210 AGATCGGAAGAGA 208 AGATCGGAAGAGCA 208 AGATCGGAAGAGCGA 201 AGATCGGAAGAGCGTA 181 AGATCGGAAGAGCGTCA 206 AGATCGGAAGAGCGTCGA 187 AGATCGGAAGAGCGTCGTA 162 AGATCGGAAGAGCGTCGTGA 205 AGATCGGAAGAGCGTCGTGTA 226 AGATCGGAAGAGCGTCGTGTAA 181 AGATCGGAAGAGCGTCGTGTAGA 192 AGATCGGAAGAGCGTCGTGTAGGA 174 AGATCGGAAGAGCGTCGTGTAGGGA 157 AGATCGGAAGAGCGTCGTGTAGGGAA 204 AGATCGGAAGAGCGTCGTGTAGGGAAA 182 AGATCGGAAGAGCGTCGTGTAGGGAAAA 200 AGATCGGAAGAGCGTCGTGTAGGGAAAGA 180 AGATCGGAAGAGCGTCGTGTAGGGAAAGAA 178 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGA 170 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTA 192 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGA 174 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCA 158 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGA 165 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGTA 161 other adapter sequences 7762

Insert size estimation

This estimation is based on paired-end overlap analysis, and there are 36.276941% reads found not overlapped.
The nonoverlapped read pairs may have insert size <30 or >270, or contain too much sequencing errors to be detected as overlapped.

Filtering statistics

Before filtering: read1: quality Value of each position will be shown on mouse over.	After filtering: read1: quality Value of each position will be shown on mouse over.
Before filtering: read2: quality Value of each position will be shown on mouse over.	After filtering: read2: quality Value of each position will be shown on mouse over.
Before filtering: read1: base contents Value of each position will be shown on mouse over.	After filtering: read1: base contents Value of each position will be shown on mouse over.
Before filtering: read2: base contents Value of each position will be shown on mouse over.	After filtering: read2: base contents Value of each position will be shown on mouse over.
Before filtering: read1: KMER counting Darker background means larger counts. The count will be shown on mouse over. AA AT AC AG TA TT TC TG CA CT CC CG GA GT GC GG AAA AAAAA AAAAT AAAAC AAAAG AAATA AAATT AAATC AAATG AAACA AAACT AAACC AAACG AAAGA AAAGT AAAGC AAAGG AAT AATAA AATAT AATAC AATAG AATTA AATTT AATTC AATTG AATCA AATCT AATCC AATCG AATGA AATGT AATGC AATGG AAC AACAA AACAT AACAC AACAG AACTA AACTT AACTC AACTG AACCA AACCT AACCC AACCG AACGA AACGT AACGC AACGG AAG AAGAA AAGAT AAGAC AAGAG AAGTA AAGTT AAGTC AAGTG AAGCA AAGCT AAGCC AAGCG AAGGA AAGGT AAGGC AAGGG ATA ATAAA ATAAT ATAAC ATAAG ATATA ATATT ATATC ATATG ATACA ATACT ATACC ATACG ATAGA ATAGT ATAGC ATAGG ATT ATTAA ATTAT ATTAC ATTAG ATTTA ATTTT ATTTC ATTTG ATTCA ATTCT ATTCC ATTCG ATTGA ATTGT ATTGC ATTGG ATC ATCAA ATCAT ATCAC ATCAG ATCTA ATCTT ATCTC ATCTG ATCCA ATCCT ATCCC ATCCG ATCGA ATCGT ATCGC ATCGG ATG ATGAA ATGAT ATGAC ATGAG ATGTA ATGTT ATGTC ATGTG ATGCA ATGCT ATGCC ATGCG ATGGA ATGGT ATGGC ATGGG ACA ACAAA ACAAT ACAAC ACAAG ACATA ACATT ACATC ACATG ACACA ACACT ACACC ACACG ACAGA ACAGT ACAGC ACAGG ACT ACTAA ACTAT ACTAC ACTAG ACTTA ACTTT ACTTC ACTTG ACTCA ACTCT ACTCC ACTCG ACTGA ACTGT ACTGC ACTGG ACC ACCAA ACCAT ACCAC ACCAG ACCTA ACCTT ACCTC ACCTG ACCCA ACCCT ACCCC ACCCG ACCGA ACCGT ACCGC ACCGG ACG ACGAA ACGAT ACGAC ACGAG ACGTA ACGTT ACGTC ACGTG ACGCA ACGCT ACGCC ACGCG ACGGA ACGGT ACGGC ACGGG AGA AGAAA AGAAT AGAAC AGAAG AGATA AGATT AGATC AGATG AGACA AGACT AGACC AGACG AGAGA AGAGT AGAGC AGAGG AGT AGTAA AGTAT AGTAC AGTAG AGTTA AGTTT AGTTC AGTTG AGTCA AGTCT AGTCC AGTCG AGTGA AGTGT AGTGC AGTGG AGC AGCAA AGCAT AGCAC AGCAG AGCTA AGCTT AGCTC AGCTG AGCCA AGCCT AGCCC AGCCG AGCGA AGCGT AGCGC AGCGG AGG AGGAA AGGAT AGGAC AGGAG AGGTA AGGTT AGGTC AGGTG AGGCA AGGCT AGGCC AGGCG AGGGA AGGGT AGGGC AGGGG TAA TAAAA TAAAT TAAAC TAAAG TAATA TAATT TAATC TAATG TAACA TAACT TAACC TAACG TAAGA TAAGT TAAGC TAAGG TAT TATAA TATAT TATAC TATAG TATTA TATTT TATTC TATTG TATCA TATCT TATCC TATCG TATGA TATGT TATGC TATGG TAC TACAA TACAT TACAC TACAG TACTA TACTT TACTC TACTG TACCA TACCT TACCC TACCG TACGA TACGT TACGC TACGG TAG TAGAA TAGAT TAGAC TAGAG TAGTA TAGTT TAGTC TAGTG TAGCA TAGCT TAGCC TAGCG TAGGA TAGGT TAGGC TAGGG TTA TTAAA TTAAT TTAAC TTAAG TTATA TTATT TTATC TTATG TTACA TTACT TTACC TTACG TTAGA TTAGT TTAGC TTAGG TTT TTTAA TTTAT TTTAC TTTAG TTTTA TTTTT TTTTC TTTTG TTTCA TTTCT TTTCC TTTCG TTTGA TTTGT TTTGC TTTGG TTC TTCAA TTCAT TTCAC TTCAG TTCTA TTCTT TTCTC TTCTG TTCCA TTCCT TTCCC TTCCG TTCGA TTCGT TTCGC TTCGG TTG TTGAA TTGAT TTGAC TTGAG TTGTA TTGTT TTGTC TTGTG TTGCA TTGCT TTGCC TTGCG TTGGA TTGGT TTGGC TTGGG TCA TCAAA TCAAT TCAAC TCAAG TCATA TCATT TCATC TCATG TCACA TCACT TCACC TCACG TCAGA TCAGT TCAGC TCAGG TCT TCTAA TCTAT TCTAC TCTAG TCTTA TCTTT TCTTC TCTTG TCTCA TCTCT TCTCC TCTCG TCTGA TCTGT TCTGC TCTGG TCC TCCAA TCCAT TCCAC TCCAG TCCTA TCCTT TCCTC TCCTG TCCCA TCCCT TCCCC TCCCG TCCGA TCCGT TCCGC TCCGG TCG TCGAA TCGAT TCGAC TCGAG TCGTA TCGTT TCGTC TCGTG TCGCA TCGCT TCGCC TCGCG TCGGA TCGGT TCGGC TCGGG TGA TGAAA TGAAT TGAAC TGAAG TGATA TGATT TGATC TGATG TGACA TGACT TGACC TGACG TGAGA TGAGT TGAGC TGAGG TGT TGTAA TGTAT TGTAC TGTAG TGTTA TGTTT TGTTC TGTTG TGTCA TGTCT TGTCC TGTCG TGTGA TGTGT TGTGC TGTGG TGC TGCAA TGCAT TGCAC TGCAG TGCTA TGCTT TGCTC TGCTG TGCCA TGCCT TGCCC TGCCG TGCGA TGCGT TGCGC TGCGG TGG TGGAA TGGAT TGGAC TGGAG TGGTA TGGTT TGGTC TGGTG TGGCA TGGCT TGGCC TGGCG TGGGA TGGGT TGGGC TGGGG CAA CAAAA CAAAT CAAAC CAAAG CAATA CAATT CAATC CAATG CAACA CAACT CAACC CAACG CAAGA CAAGT CAAGC CAAGG CAT CATAA CATAT CATAC CATAG CATTA CATTT CATTC CATTG CATCA CATCT CATCC CATCG CATGA CATGT CATGC CATGG CAC CACAA CACAT CACAC CACAG CACTA CACTT CACTC CACTG CACCA CACCT CACCC CACCG CACGA CACGT CACGC CACGG CAG CAGAA CAGAT CAGAC CAGAG CAGTA CAGTT CAGTC CAGTG CAGCA CAGCT CAGCC CAGCG CAGGA CAGGT CAGGC CAGGG CTA CTAAA CTAAT CTAAC CTAAG CTATA CTATT CTATC CTATG CTACA CTACT CTACC CTACG CTAGA CTAGT CTAGC CTAGG CTT CTTAA CTTAT CTTAC CTTAG CTTTA CTTTT CTTTC CTTTG CTTCA CTTCT CTTCC CTTCG CTTGA CTTGT CTTGC CTTGG CTC CTCAA CTCAT CTCAC CTCAG CTCTA CTCTT CTCTC CTCTG CTCCA CTCCT CTCCC CTCCG CTCGA CTCGT CTCGC CTCGG CTG CTGAA CTGAT CTGAC CTGAG CTGTA CTGTT CTGTC CTGTG CTGCA CTGCT CTGCC CTGCG CTGGA CTGGT CTGGC CTGGG CCA CCAAA CCAAT CCAAC CCAAG CCATA CCATT CCATC CCATG CCACA CCACT CCACC CCACG CCAGA CCAGT CCAGC CCAGG CCT CCTAA CCTAT CCTAC CCTAG CCTTA CCTTT CCTTC CCTTG CCTCA CCTCT CCTCC CCTCG CCTGA CCTGT CCTGC CCTGG CCC CCCAA CCCAT CCCAC CCCAG CCCTA CCCTT CCCTC CCCTG CCCCA CCCCT CCCCC CCCCG CCCGA CCCGT CCCGC CCCGG CCG CCGAA CCGAT CCGAC CCGAG CCGTA CCGTT CCGTC CCGTG CCGCA CCGCT CCGCC CCGCG CCGGA CCGGT CCGGC CCGGG CGA CGAAA CGAAT CGAAC CGAAG CGATA CGATT CGATC CGATG CGACA CGACT CGACC CGACG CGAGA CGAGT CGAGC CGAGG CGT CGTAA CGTAT CGTAC CGTAG CGTTA CGTTT CGTTC CGTTG CGTCA CGTCT CGTCC CGTCG CGTGA CGTGT CGTGC CGTGG CGC CGCAA CGCAT CGCAC CGCAG CGCTA CGCTT CGCTC CGCTG CGCCA CGCCT CGCCC CGCCG CGCGA CGCGT CGCGC CGCGG CGG CGGAA CGGAT CGGAC CGGAG CGGTA CGGTT CGGTC CGGTG CGGCA CGGCT CGGCC CGGCG CGGGA CGGGT CGGGC CGGGG GAA GAAAA GAAAT GAAAC GAAAG GAATA GAATT GAATC GAATG GAACA GAACT GAACC GAACG GAAGA GAAGT GAAGC GAAGG GAT GATAA GATAT GATAC GATAG GATTA GATTT GATTC GATTG GATCA GATCT GATCC GATCG GATGA GATGT GATGC GATGG GAC GACAA GACAT GACAC GACAG GACTA GACTT GACTC GACTG GACCA GACCT GACCC GACCG GACGA GACGT GACGC GACGG GAG GAGAA GAGAT GAGAC GAGAG GAGTA GAGTT GAGTC GAGTG GAGCA GAGCT GAGCC GAGCG GAGGA GAGGT GAGGC GAGGG GTA GTAAA GTAAT GTAAC GTAAG GTATA GTATT GTATC GTATG GTACA GTACT GTACC GTACG GTAGA GTAGT GTAGC GTAGG GTT GTTAA GTTAT GTTAC GTTAG GTTTA GTTTT GTTTC GTTTG GTTCA GTTCT GTTCC GTTCG GTTGA GTTGT GTTGC GTTGG GTC GTCAA GTCAT GTCAC GTCAG GTCTA GTCTT GTCTC GTCTG GTCCA GTCCT GTCCC GTCCG GTCGA GTCGT GTCGC GTCGG GTG GTGAA GTGAT GTGAC GTGAG GTGTA GTGTT GTGTC GTGTG GTGCA GTGCT GTGCC GTGCG GTGGA GTGGT GTGGC GTGGG GCA GCAAA GCAAT GCAAC GCAAG GCATA GCATT GCATC GCATG GCACA GCACT GCACC GCACG GCAGA GCAGT GCAGC GCAGG GCT GCTAA GCTAT GCTAC GCTAG GCTTA GCTTT GCTTC GCTTG GCTCA GCTCT GCTCC GCTCG GCTGA GCTGT GCTGC GCTGG GCC GCCAA GCCAT GCCAC GCCAG GCCTA GCCTT GCCTC GCCTG GCCCA GCCCT GCCCC GCCCG GCCGA GCCGT GCCGC GCCGG GCG GCGAA GCGAT GCGAC GCGAG GCGTA GCGTT GCGTC GCGTG GCGCA GCGCT GCGCC GCGCG GCGGA GCGGT GCGGC GCGGG GGA GGAAA GGAAT GGAAC GGAAG GGATA GGATT GGATC GGATG GGACA GGACT GGACC GGACG GGAGA GGAGT GGAGC GGAGG GGT GGTAA GGTAT GGTAC GGTAG GGTTA GGTTT GGTTC GGTTG GGTCA GGTCT GGTCC GGTCG GGTGA GGTGT GGTGC GGTGG GGC GGCAA GGCAT GGCAC GGCAG GGCTA GGCTT GGCTC GGCTG GGCCA GGCCT GGCCC GGCCG GGCGA GGCGT GGCGC GGCGG GGG GGGAA GGGAT GGGAC GGGAG GGGTA GGGTT GGGTC GGGTG GGGCA GGGCT GGGCC GGGCG GGGGA GGGGT GGGGC GGGGG	After filtering: read1: KMER counting Darker background means larger counts. The count will be shown on mouse over. AA AT AC AG TA TT TC TG CA CT CC CG GA GT GC GG AAA AAAAA AAAAT AAAAC AAAAG AAATA AAATT AAATC AAATG AAACA AAACT AAACC AAACG AAAGA AAAGT AAAGC AAAGG AAT AATAA AATAT AATAC AATAG AATTA AATTT AATTC AATTG AATCA AATCT AATCC AATCG AATGA AATGT AATGC AATGG AAC AACAA AACAT AACAC AACAG AACTA AACTT AACTC AACTG AACCA AACCT AACCC AACCG AACGA AACGT AACGC AACGG AAG AAGAA AAGAT AAGAC AAGAG AAGTA AAGTT AAGTC AAGTG AAGCA AAGCT AAGCC AAGCG AAGGA AAGGT AAGGC AAGGG ATA ATAAA ATAAT ATAAC ATAAG ATATA ATATT ATATC ATATG ATACA ATACT ATACC ATACG ATAGA ATAGT ATAGC ATAGG ATT ATTAA ATTAT ATTAC ATTAG ATTTA ATTTT ATTTC ATTTG ATTCA ATTCT ATTCC ATTCG ATTGA ATTGT ATTGC ATTGG ATC ATCAA ATCAT ATCAC ATCAG ATCTA ATCTT ATCTC ATCTG ATCCA ATCCT ATCCC ATCCG ATCGA ATCGT ATCGC ATCGG ATG ATGAA ATGAT ATGAC ATGAG ATGTA ATGTT ATGTC ATGTG ATGCA ATGCT ATGCC ATGCG ATGGA ATGGT ATGGC ATGGG ACA ACAAA ACAAT ACAAC ACAAG ACATA ACATT ACATC ACATG ACACA ACACT ACACC ACACG ACAGA ACAGT ACAGC ACAGG ACT ACTAA ACTAT ACTAC ACTAG ACTTA ACTTT ACTTC ACTTG ACTCA ACTCT ACTCC ACTCG ACTGA ACTGT ACTGC ACTGG ACC ACCAA ACCAT ACCAC ACCAG ACCTA ACCTT ACCTC ACCTG ACCCA ACCCT ACCCC ACCCG ACCGA ACCGT ACCGC ACCGG ACG ACGAA ACGAT ACGAC ACGAG ACGTA ACGTT ACGTC ACGTG ACGCA ACGCT ACGCC ACGCG ACGGA ACGGT ACGGC ACGGG AGA AGAAA AGAAT AGAAC AGAAG AGATA AGATT AGATC AGATG AGACA AGACT AGACC AGACG AGAGA AGAGT AGAGC AGAGG AGT AGTAA AGTAT AGTAC AGTAG AGTTA AGTTT AGTTC AGTTG AGTCA AGTCT AGTCC AGTCG AGTGA AGTGT AGTGC AGTGG AGC AGCAA AGCAT AGCAC AGCAG AGCTA AGCTT AGCTC AGCTG AGCCA AGCCT AGCCC AGCCG AGCGA AGCGT AGCGC AGCGG AGG AGGAA AGGAT AGGAC AGGAG AGGTA AGGTT AGGTC AGGTG AGGCA AGGCT AGGCC AGGCG AGGGA AGGGT AGGGC AGGGG TAA TAAAA TAAAT TAAAC TAAAG TAATA TAATT TAATC TAATG TAACA TAACT TAACC TAACG TAAGA TAAGT TAAGC TAAGG TAT TATAA TATAT TATAC TATAG TATTA TATTT TATTC TATTG TATCA TATCT TATCC TATCG TATGA TATGT TATGC TATGG TAC TACAA TACAT TACAC TACAG TACTA TACTT TACTC TACTG TACCA TACCT TACCC TACCG TACGA TACGT TACGC TACGG TAG TAGAA TAGAT TAGAC TAGAG TAGTA TAGTT TAGTC TAGTG TAGCA TAGCT TAGCC TAGCG TAGGA TAGGT TAGGC TAGGG TTA TTAAA TTAAT TTAAC TTAAG TTATA TTATT TTATC TTATG TTACA TTACT TTACC TTACG TTAGA TTAGT TTAGC TTAGG TTT TTTAA TTTAT TTTAC TTTAG TTTTA TTTTT TTTTC TTTTG TTTCA TTTCT TTTCC TTTCG TTTGA TTTGT TTTGC TTTGG TTC TTCAA TTCAT TTCAC TTCAG TTCTA TTCTT TTCTC TTCTG TTCCA TTCCT TTCCC TTCCG TTCGA TTCGT TTCGC TTCGG TTG TTGAA TTGAT TTGAC TTGAG TTGTA TTGTT TTGTC TTGTG TTGCA TTGCT TTGCC TTGCG TTGGA TTGGT TTGGC TTGGG TCA TCAAA TCAAT TCAAC TCAAG TCATA TCATT TCATC TCATG TCACA TCACT TCACC TCACG TCAGA TCAGT TCAGC TCAGG TCT TCTAA TCTAT TCTAC TCTAG TCTTA TCTTT TCTTC TCTTG TCTCA TCTCT TCTCC TCTCG TCTGA TCTGT TCTGC TCTGG TCC TCCAA TCCAT TCCAC TCCAG TCCTA TCCTT TCCTC TCCTG TCCCA TCCCT TCCCC TCCCG TCCGA TCCGT TCCGC TCCGG TCG TCGAA TCGAT TCGAC TCGAG TCGTA TCGTT TCGTC TCGTG TCGCA TCGCT TCGCC TCGCG TCGGA TCGGT TCGGC TCGGG TGA TGAAA TGAAT TGAAC TGAAG TGATA TGATT TGATC TGATG TGACA TGACT TGACC TGACG TGAGA TGAGT TGAGC TGAGG TGT TGTAA TGTAT TGTAC TGTAG TGTTA TGTTT TGTTC TGTTG TGTCA TGTCT TGTCC TGTCG TGTGA TGTGT TGTGC TGTGG TGC TGCAA TGCAT TGCAC TGCAG TGCTA TGCTT TGCTC TGCTG TGCCA TGCCT TGCCC TGCCG TGCGA TGCGT TGCGC TGCGG TGG TGGAA TGGAT TGGAC TGGAG TGGTA TGGTT TGGTC TGGTG TGGCA TGGCT TGGCC TGGCG TGGGA TGGGT TGGGC TGGGG CAA CAAAA CAAAT CAAAC CAAAG CAATA CAATT CAATC CAATG CAACA CAACT CAACC CAACG CAAGA CAAGT CAAGC CAAGG CAT CATAA CATAT CATAC CATAG CATTA CATTT CATTC CATTG CATCA CATCT CATCC CATCG CATGA CATGT CATGC CATGG CAC CACAA CACAT CACAC CACAG CACTA CACTT CACTC CACTG CACCA CACCT CACCC CACCG CACGA CACGT CACGC CACGG CAG CAGAA CAGAT CAGAC CAGAG CAGTA CAGTT CAGTC CAGTG CAGCA CAGCT CAGCC CAGCG CAGGA CAGGT CAGGC CAGGG CTA CTAAA CTAAT CTAAC CTAAG CTATA CTATT CTATC CTATG CTACA CTACT CTACC CTACG CTAGA CTAGT CTAGC CTAGG CTT CTTAA CTTAT CTTAC CTTAG CTTTA CTTTT CTTTC CTTTG CTTCA CTTCT CTTCC CTTCG CTTGA CTTGT CTTGC CTTGG CTC CTCAA CTCAT CTCAC CTCAG CTCTA CTCTT CTCTC CTCTG CTCCA CTCCT CTCCC CTCCG CTCGA CTCGT CTCGC CTCGG CTG CTGAA CTGAT CTGAC CTGAG CTGTA CTGTT CTGTC CTGTG CTGCA CTGCT CTGCC CTGCG CTGGA CTGGT CTGGC CTGGG CCA CCAAA CCAAT CCAAC CCAAG CCATA CCATT CCATC CCATG CCACA CCACT CCACC CCACG CCAGA CCAGT CCAGC CCAGG CCT CCTAA CCTAT CCTAC CCTAG CCTTA CCTTT CCTTC CCTTG CCTCA CCTCT CCTCC CCTCG CCTGA CCTGT CCTGC CCTGG CCC CCCAA CCCAT CCCAC CCCAG CCCTA CCCTT CCCTC CCCTG CCCCA CCCCT CCCCC CCCCG CCCGA CCCGT CCCGC CCCGG CCG CCGAA CCGAT CCGAC CCGAG CCGTA CCGTT CCGTC CCGTG CCGCA CCGCT CCGCC CCGCG CCGGA CCGGT CCGGC CCGGG CGA CGAAA CGAAT CGAAC CGAAG CGATA CGATT CGATC CGATG CGACA CGACT CGACC CGACG CGAGA CGAGT CGAGC CGAGG CGT CGTAA CGTAT CGTAC CGTAG CGTTA CGTTT CGTTC CGTTG CGTCA CGTCT CGTCC CGTCG CGTGA CGTGT CGTGC CGTGG CGC CGCAA CGCAT CGCAC CGCAG CGCTA CGCTT CGCTC CGCTG CGCCA CGCCT CGCCC CGCCG CGCGA CGCGT CGCGC CGCGG CGG CGGAA CGGAT CGGAC CGGAG CGGTA CGGTT CGGTC CGGTG CGGCA CGGCT CGGCC CGGCG CGGGA CGGGT CGGGC CGGGG GAA GAAAA GAAAT GAAAC GAAAG GAATA GAATT GAATC GAATG GAACA GAACT GAACC GAACG GAAGA GAAGT GAAGC GAAGG GAT GATAA GATAT GATAC GATAG GATTA GATTT GATTC GATTG GATCA GATCT GATCC GATCG GATGA GATGT GATGC GATGG GAC GACAA GACAT GACAC GACAG GACTA GACTT GACTC GACTG GACCA GACCT GACCC GACCG GACGA GACGT GACGC GACGG GAG GAGAA GAGAT GAGAC GAGAG GAGTA GAGTT GAGTC GAGTG GAGCA GAGCT GAGCC GAGCG GAGGA GAGGT GAGGC GAGGG GTA GTAAA GTAAT GTAAC GTAAG GTATA GTATT GTATC GTATG GTACA GTACT GTACC GTACG GTAGA GTAGT GTAGC GTAGG GTT GTTAA GTTAT GTTAC GTTAG GTTTA GTTTT GTTTC GTTTG GTTCA GTTCT GTTCC GTTCG GTTGA GTTGT GTTGC GTTGG GTC GTCAA GTCAT GTCAC GTCAG GTCTA GTCTT GTCTC GTCTG GTCCA GTCCT GTCCC GTCCG GTCGA GTCGT GTCGC GTCGG GTG GTGAA GTGAT GTGAC GTGAG GTGTA GTGTT GTGTC GTGTG GTGCA GTGCT GTGCC GTGCG GTGGA GTGGT GTGGC GTGGG GCA GCAAA GCAAT GCAAC GCAAG GCATA GCATT GCATC GCATG GCACA GCACT GCACC GCACG GCAGA GCAGT GCAGC GCAGG GCT GCTAA GCTAT GCTAC GCTAG GCTTA GCTTT GCTTC GCTTG GCTCA GCTCT GCTCC GCTCG GCTGA GCTGT GCTGC GCTGG GCC GCCAA GCCAT GCCAC GCCAG GCCTA GCCTT GCCTC GCCTG GCCCA GCCCT GCCCC GCCCG GCCGA GCCGT GCCGC GCCGG GCG GCGAA GCGAT GCGAC GCGAG GCGTA GCGTT GCGTC GCGTG GCGCA GCGCT GCGCC GCGCG GCGGA GCGGT GCGGC GCGGG GGA GGAAA GGAAT GGAAC GGAAG GGATA GGATT GGATC GGATG GGACA GGACT GGACC GGACG GGAGA GGAGT GGAGC GGAGG GGT GGTAA GGTAT GGTAC GGTAG GGTTA GGTTT GGTTC GGTTG GGTCA GGTCT GGTCC GGTCG GGTGA GGTGT GGTGC GGTGG GGC GGCAA GGCAT GGCAC GGCAG GGCTA GGCTT GGCTC GGCTG GGCCA GGCCT GGCCC GGCCG GGCGA GGCGT GGCGC GGCGG GGG GGGAA GGGAT GGGAC GGGAG GGGTA GGGTT GGGTC GGGTG GGGCA GGGCT GGGCC GGGCG GGGGA GGGGT GGGGC GGGGG
Before filtering: read2: KMER counting Darker background means larger counts. The count will be shown on mouse over. AA AT AC AG TA TT TC TG CA CT CC CG GA GT GC GG AAA AAAAA AAAAT AAAAC AAAAG AAATA AAATT AAATC AAATG AAACA AAACT AAACC AAACG AAAGA AAAGT AAAGC AAAGG AAT AATAA AATAT AATAC AATAG AATTA AATTT AATTC AATTG AATCA AATCT AATCC AATCG AATGA AATGT AATGC AATGG AAC AACAA AACAT AACAC AACAG AACTA AACTT AACTC AACTG AACCA AACCT AACCC AACCG AACGA AACGT AACGC AACGG AAG AAGAA AAGAT AAGAC AAGAG AAGTA AAGTT AAGTC AAGTG AAGCA AAGCT AAGCC AAGCG AAGGA AAGGT AAGGC AAGGG ATA ATAAA ATAAT ATAAC ATAAG ATATA ATATT ATATC ATATG ATACA ATACT ATACC ATACG ATAGA ATAGT ATAGC ATAGG ATT ATTAA ATTAT ATTAC ATTAG ATTTA ATTTT ATTTC ATTTG ATTCA ATTCT ATTCC ATTCG ATTGA ATTGT ATTGC ATTGG ATC ATCAA ATCAT ATCAC ATCAG ATCTA ATCTT ATCTC ATCTG ATCCA ATCCT ATCCC ATCCG ATCGA ATCGT ATCGC ATCGG ATG ATGAA ATGAT ATGAC ATGAG ATGTA ATGTT ATGTC ATGTG ATGCA ATGCT ATGCC ATGCG ATGGA ATGGT ATGGC ATGGG ACA ACAAA ACAAT ACAAC ACAAG ACATA ACATT ACATC ACATG ACACA ACACT ACACC ACACG ACAGA ACAGT ACAGC ACAGG ACT ACTAA ACTAT ACTAC ACTAG ACTTA ACTTT ACTTC ACTTG ACTCA ACTCT ACTCC ACTCG ACTGA ACTGT ACTGC ACTGG ACC ACCAA ACCAT ACCAC ACCAG ACCTA ACCTT ACCTC ACCTG ACCCA ACCCT ACCCC ACCCG ACCGA ACCGT ACCGC ACCGG ACG ACGAA ACGAT ACGAC ACGAG ACGTA ACGTT ACGTC ACGTG ACGCA ACGCT ACGCC ACGCG ACGGA ACGGT ACGGC ACGGG AGA AGAAA AGAAT AGAAC AGAAG AGATA AGATT AGATC AGATG AGACA AGACT AGACC AGACG AGAGA AGAGT AGAGC AGAGG AGT AGTAA AGTAT AGTAC AGTAG AGTTA AGTTT AGTTC AGTTG AGTCA AGTCT AGTCC AGTCG AGTGA AGTGT AGTGC AGTGG AGC AGCAA AGCAT AGCAC AGCAG AGCTA AGCTT AGCTC AGCTG AGCCA AGCCT AGCCC AGCCG AGCGA AGCGT AGCGC AGCGG AGG AGGAA AGGAT AGGAC AGGAG AGGTA AGGTT AGGTC AGGTG AGGCA AGGCT AGGCC AGGCG AGGGA AGGGT AGGGC AGGGG TAA TAAAA TAAAT TAAAC TAAAG TAATA TAATT TAATC TAATG TAACA TAACT TAACC TAACG TAAGA TAAGT TAAGC TAAGG TAT TATAA TATAT TATAC TATAG TATTA TATTT TATTC TATTG TATCA TATCT TATCC TATCG TATGA TATGT TATGC TATGG TAC TACAA TACAT TACAC TACAG TACTA TACTT TACTC TACTG TACCA TACCT TACCC TACCG TACGA TACGT TACGC TACGG TAG TAGAA TAGAT TAGAC TAGAG TAGTA TAGTT TAGTC TAGTG TAGCA TAGCT TAGCC TAGCG TAGGA TAGGT TAGGC TAGGG TTA TTAAA TTAAT TTAAC TTAAG TTATA TTATT TTATC TTATG TTACA TTACT TTACC TTACG TTAGA TTAGT TTAGC TTAGG TTT TTTAA TTTAT TTTAC TTTAG TTTTA TTTTT TTTTC TTTTG TTTCA TTTCT TTTCC TTTCG TTTGA TTTGT TTTGC TTTGG TTC TTCAA TTCAT TTCAC TTCAG TTCTA TTCTT TTCTC TTCTG TTCCA TTCCT TTCCC TTCCG TTCGA TTCGT TTCGC TTCGG TTG TTGAA TTGAT TTGAC TTGAG TTGTA TTGTT TTGTC TTGTG TTGCA TTGCT TTGCC TTGCG TTGGA TTGGT TTGGC TTGGG TCA TCAAA TCAAT TCAAC TCAAG TCATA TCATT TCATC TCATG TCACA TCACT TCACC TCACG TCAGA TCAGT TCAGC TCAGG TCT TCTAA TCTAT TCTAC TCTAG TCTTA TCTTT TCTTC TCTTG TCTCA TCTCT TCTCC TCTCG TCTGA TCTGT TCTGC TCTGG TCC TCCAA TCCAT TCCAC TCCAG TCCTA TCCTT TCCTC TCCTG TCCCA TCCCT TCCCC TCCCG TCCGA TCCGT TCCGC TCCGG TCG TCGAA TCGAT TCGAC TCGAG TCGTA TCGTT TCGTC TCGTG TCGCA TCGCT TCGCC TCGCG TCGGA TCGGT TCGGC TCGGG TGA TGAAA TGAAT TGAAC TGAAG TGATA TGATT TGATC TGATG TGACA TGACT TGACC TGACG TGAGA TGAGT TGAGC TGAGG TGT TGTAA TGTAT TGTAC TGTAG TGTTA TGTTT TGTTC TGTTG TGTCA TGTCT TGTCC TGTCG TGTGA TGTGT TGTGC TGTGG TGC TGCAA TGCAT TGCAC TGCAG TGCTA TGCTT TGCTC TGCTG TGCCA TGCCT TGCCC TGCCG TGCGA TGCGT TGCGC TGCGG TGG TGGAA TGGAT TGGAC TGGAG TGGTA TGGTT TGGTC TGGTG TGGCA TGGCT TGGCC TGGCG TGGGA TGGGT TGGGC TGGGG CAA CAAAA CAAAT CAAAC CAAAG CAATA CAATT CAATC CAATG CAACA CAACT CAACC CAACG CAAGA CAAGT CAAGC CAAGG CAT CATAA CATAT CATAC CATAG CATTA CATTT CATTC CATTG CATCA CATCT CATCC CATCG CATGA CATGT CATGC CATGG CAC CACAA CACAT CACAC CACAG CACTA CACTT CACTC CACTG CACCA CACCT CACCC CACCG CACGA CACGT CACGC CACGG CAG CAGAA CAGAT CAGAC CAGAG CAGTA CAGTT CAGTC CAGTG CAGCA CAGCT CAGCC CAGCG CAGGA CAGGT CAGGC CAGGG CTA CTAAA CTAAT CTAAC CTAAG CTATA CTATT CTATC CTATG CTACA CTACT CTACC CTACG CTAGA CTAGT CTAGC CTAGG CTT CTTAA CTTAT CTTAC CTTAG CTTTA CTTTT CTTTC CTTTG CTTCA CTTCT CTTCC CTTCG CTTGA CTTGT CTTGC CTTGG CTC CTCAA CTCAT CTCAC CTCAG CTCTA CTCTT CTCTC CTCTG CTCCA CTCCT CTCCC CTCCG CTCGA CTCGT CTCGC CTCGG CTG CTGAA CTGAT CTGAC CTGAG CTGTA CTGTT CTGTC CTGTG CTGCA CTGCT CTGCC CTGCG CTGGA CTGGT CTGGC CTGGG CCA CCAAA CCAAT CCAAC CCAAG CCATA CCATT CCATC CCATG CCACA CCACT CCACC CCACG CCAGA CCAGT CCAGC CCAGG CCT CCTAA CCTAT CCTAC CCTAG CCTTA CCTTT CCTTC CCTTG CCTCA CCTCT CCTCC CCTCG CCTGA CCTGT CCTGC CCTGG CCC CCCAA CCCAT CCCAC CCCAG CCCTA CCCTT CCCTC CCCTG CCCCA CCCCT CCCCC CCCCG CCCGA CCCGT CCCGC CCCGG CCG CCGAA CCGAT CCGAC CCGAG CCGTA CCGTT CCGTC CCGTG CCGCA CCGCT CCGCC CCGCG CCGGA CCGGT CCGGC CCGGG CGA CGAAA CGAAT CGAAC CGAAG CGATA CGATT CGATC CGATG CGACA CGACT CGACC CGACG CGAGA CGAGT CGAGC CGAGG CGT CGTAA CGTAT CGTAC CGTAG CGTTA CGTTT CGTTC CGTTG CGTCA CGTCT CGTCC CGTCG CGTGA CGTGT CGTGC CGTGG CGC CGCAA CGCAT CGCAC CGCAG CGCTA CGCTT CGCTC CGCTG CGCCA CGCCT CGCCC CGCCG CGCGA CGCGT CGCGC CGCGG CGG CGGAA CGGAT CGGAC CGGAG CGGTA CGGTT CGGTC CGGTG CGGCA CGGCT CGGCC CGGCG CGGGA CGGGT CGGGC CGGGG GAA GAAAA GAAAT GAAAC GAAAG GAATA GAATT GAATC GAATG GAACA GAACT GAACC GAACG GAAGA GAAGT GAAGC GAAGG GAT GATAA GATAT GATAC GATAG GATTA GATTT GATTC GATTG GATCA GATCT GATCC GATCG GATGA GATGT GATGC GATGG GAC GACAA GACAT GACAC GACAG GACTA GACTT GACTC GACTG GACCA GACCT GACCC GACCG GACGA GACGT GACGC GACGG GAG GAGAA GAGAT GAGAC GAGAG GAGTA GAGTT GAGTC GAGTG GAGCA GAGCT GAGCC GAGCG GAGGA GAGGT GAGGC GAGGG GTA GTAAA GTAAT GTAAC GTAAG GTATA GTATT GTATC GTATG GTACA GTACT GTACC GTACG GTAGA GTAGT GTAGC GTAGG GTT GTTAA GTTAT GTTAC GTTAG GTTTA GTTTT GTTTC GTTTG GTTCA GTTCT GTTCC GTTCG GTTGA GTTGT GTTGC GTTGG GTC GTCAA GTCAT GTCAC GTCAG GTCTA GTCTT GTCTC GTCTG GTCCA GTCCT GTCCC GTCCG GTCGA GTCGT GTCGC GTCGG GTG GTGAA GTGAT GTGAC GTGAG GTGTA GTGTT GTGTC GTGTG GTGCA GTGCT GTGCC GTGCG GTGGA GTGGT GTGGC GTGGG GCA GCAAA GCAAT GCAAC GCAAG GCATA GCATT GCATC GCATG GCACA GCACT GCACC GCACG GCAGA GCAGT GCAGC GCAGG GCT GCTAA GCTAT GCTAC GCTAG GCTTA GCTTT GCTTC GCTTG GCTCA GCTCT GCTCC GCTCG GCTGA GCTGT GCTGC GCTGG GCC GCCAA GCCAT GCCAC GCCAG GCCTA GCCTT GCCTC GCCTG GCCCA GCCCT GCCCC GCCCG GCCGA GCCGT GCCGC GCCGG GCG GCGAA GCGAT GCGAC GCGAG GCGTA GCGTT GCGTC GCGTG GCGCA GCGCT GCGCC GCGCG GCGGA GCGGT GCGGC GCGGG GGA GGAAA GGAAT GGAAC GGAAG GGATA GGATT GGATC GGATG GGACA GGACT GGACC GGACG GGAGA GGAGT GGAGC GGAGG GGT GGTAA GGTAT GGTAC GGTAG GGTTA GGTTT GGTTC GGTTG GGTCA GGTCT GGTCC GGTCG GGTGA GGTGT GGTGC GGTGG GGC GGCAA GGCAT GGCAC GGCAG GGCTA GGCTT GGCTC GGCTG GGCCA GGCCT GGCCC GGCCG GGCGA GGCGT GGCGC GGCGG GGG GGGAA GGGAT GGGAC GGGAG GGGTA GGGTT GGGTC GGGTG GGGCA GGGCT GGGCC GGGCG GGGGA GGGGT GGGGC GGGGG	After filtering: read2: KMER counting Darker background means larger counts. The count will be shown on mouse over. AA AT AC AG TA TT TC TG CA CT CC CG GA GT GC GG AAA AAAAA AAAAT AAAAC AAAAG AAATA AAATT AAATC AAATG AAACA AAACT AAACC AAACG AAAGA AAAGT AAAGC AAAGG AAT AATAA AATAT AATAC AATAG AATTA AATTT AATTC AATTG AATCA AATCT AATCC AATCG AATGA AATGT AATGC AATGG AAC AACAA AACAT AACAC AACAG AACTA AACTT AACTC AACTG AACCA AACCT AACCC AACCG AACGA AACGT AACGC AACGG AAG AAGAA AAGAT AAGAC AAGAG AAGTA AAGTT AAGTC AAGTG AAGCA AAGCT AAGCC AAGCG AAGGA AAGGT AAGGC AAGGG ATA ATAAA ATAAT ATAAC ATAAG ATATA ATATT ATATC ATATG ATACA ATACT ATACC ATACG ATAGA ATAGT ATAGC ATAGG ATT ATTAA ATTAT ATTAC ATTAG ATTTA ATTTT ATTTC ATTTG ATTCA ATTCT ATTCC ATTCG ATTGA ATTGT ATTGC ATTGG ATC ATCAA ATCAT ATCAC ATCAG ATCTA ATCTT ATCTC ATCTG ATCCA ATCCT ATCCC ATCCG ATCGA ATCGT ATCGC ATCGG ATG ATGAA ATGAT ATGAC ATGAG ATGTA ATGTT ATGTC ATGTG ATGCA ATGCT ATGCC ATGCG ATGGA ATGGT ATGGC ATGGG ACA ACAAA ACAAT ACAAC ACAAG ACATA ACATT ACATC ACATG ACACA ACACT ACACC ACACG ACAGA ACAGT ACAGC ACAGG ACT ACTAA ACTAT ACTAC ACTAG ACTTA ACTTT ACTTC ACTTG ACTCA ACTCT ACTCC ACTCG ACTGA ACTGT ACTGC ACTGG ACC ACCAA ACCAT ACCAC ACCAG ACCTA ACCTT ACCTC ACCTG ACCCA ACCCT ACCCC ACCCG ACCGA ACCGT ACCGC ACCGG ACG ACGAA ACGAT ACGAC ACGAG ACGTA ACGTT ACGTC ACGTG ACGCA ACGCT ACGCC ACGCG ACGGA ACGGT ACGGC ACGGG AGA AGAAA AGAAT AGAAC AGAAG AGATA AGATT AGATC AGATG AGACA AGACT AGACC AGACG AGAGA AGAGT AGAGC AGAGG AGT AGTAA AGTAT AGTAC AGTAG AGTTA AGTTT AGTTC AGTTG AGTCA AGTCT AGTCC AGTCG AGTGA AGTGT AGTGC AGTGG AGC AGCAA AGCAT AGCAC AGCAG AGCTA AGCTT AGCTC AGCTG AGCCA AGCCT AGCCC AGCCG AGCGA AGCGT AGCGC AGCGG AGG AGGAA AGGAT AGGAC AGGAG AGGTA AGGTT AGGTC AGGTG AGGCA AGGCT AGGCC AGGCG AGGGA AGGGT AGGGC AGGGG TAA TAAAA TAAAT TAAAC TAAAG TAATA TAATT TAATC TAATG TAACA TAACT TAACC TAACG TAAGA TAAGT TAAGC TAAGG TAT TATAA TATAT TATAC TATAG TATTA TATTT TATTC TATTG TATCA TATCT TATCC TATCG TATGA TATGT TATGC TATGG TAC TACAA TACAT TACAC TACAG TACTA TACTT TACTC TACTG TACCA TACCT TACCC TACCG TACGA TACGT TACGC TACGG TAG TAGAA TAGAT TAGAC TAGAG TAGTA TAGTT TAGTC TAGTG TAGCA TAGCT TAGCC TAGCG TAGGA TAGGT TAGGC TAGGG TTA TTAAA TTAAT TTAAC TTAAG TTATA TTATT TTATC TTATG TTACA TTACT TTACC TTACG TTAGA TTAGT TTAGC TTAGG TTT TTTAA TTTAT TTTAC TTTAG TTTTA TTTTT TTTTC TTTTG TTTCA TTTCT TTTCC TTTCG TTTGA TTTGT TTTGC TTTGG TTC TTCAA TTCAT TTCAC TTCAG TTCTA TTCTT TTCTC TTCTG TTCCA TTCCT TTCCC TTCCG TTCGA TTCGT TTCGC TTCGG TTG TTGAA TTGAT TTGAC TTGAG TTGTA TTGTT TTGTC TTGTG TTGCA TTGCT TTGCC TTGCG TTGGA TTGGT TTGGC TTGGG TCA TCAAA TCAAT TCAAC TCAAG TCATA TCATT TCATC TCATG TCACA TCACT TCACC TCACG TCAGA TCAGT TCAGC TCAGG TCT TCTAA TCTAT TCTAC TCTAG TCTTA TCTTT TCTTC TCTTG TCTCA TCTCT TCTCC TCTCG TCTGA TCTGT TCTGC TCTGG TCC TCCAA TCCAT TCCAC TCCAG TCCTA TCCTT TCCTC TCCTG TCCCA TCCCT TCCCC TCCCG TCCGA TCCGT TCCGC TCCGG TCG TCGAA TCGAT TCGAC TCGAG TCGTA TCGTT TCGTC TCGTG TCGCA TCGCT TCGCC TCGCG TCGGA TCGGT TCGGC TCGGG TGA TGAAA TGAAT TGAAC TGAAG TGATA TGATT TGATC TGATG TGACA TGACT TGACC TGACG TGAGA TGAGT TGAGC TGAGG TGT TGTAA TGTAT TGTAC TGTAG TGTTA TGTTT TGTTC TGTTG TGTCA TGTCT TGTCC TGTCG TGTGA TGTGT TGTGC TGTGG TGC TGCAA TGCAT TGCAC TGCAG TGCTA TGCTT TGCTC TGCTG TGCCA TGCCT TGCCC TGCCG TGCGA TGCGT TGCGC TGCGG TGG TGGAA TGGAT TGGAC TGGAG TGGTA TGGTT TGGTC TGGTG TGGCA TGGCT TGGCC TGGCG TGGGA TGGGT TGGGC TGGGG CAA CAAAA CAAAT CAAAC CAAAG CAATA CAATT CAATC CAATG CAACA CAACT CAACC CAACG CAAGA CAAGT CAAGC CAAGG CAT CATAA CATAT CATAC CATAG CATTA CATTT CATTC CATTG CATCA CATCT CATCC CATCG CATGA CATGT CATGC CATGG CAC CACAA CACAT CACAC CACAG CACTA CACTT CACTC CACTG CACCA CACCT CACCC CACCG CACGA CACGT CACGC CACGG CAG CAGAA CAGAT CAGAC CAGAG CAGTA CAGTT CAGTC CAGTG CAGCA CAGCT CAGCC CAGCG CAGGA CAGGT CAGGC CAGGG CTA CTAAA CTAAT CTAAC CTAAG CTATA CTATT CTATC CTATG CTACA CTACT CTACC CTACG CTAGA CTAGT CTAGC CTAGG CTT CTTAA CTTAT CTTAC CTTAG CTTTA CTTTT CTTTC CTTTG CTTCA CTTCT CTTCC CTTCG CTTGA CTTGT CTTGC CTTGG CTC CTCAA CTCAT CTCAC CTCAG CTCTA CTCTT CTCTC CTCTG CTCCA CTCCT CTCCC CTCCG CTCGA CTCGT CTCGC CTCGG CTG CTGAA CTGAT CTGAC CTGAG CTGTA CTGTT CTGTC CTGTG CTGCA CTGCT CTGCC CTGCG CTGGA CTGGT CTGGC CTGGG CCA CCAAA CCAAT CCAAC CCAAG CCATA CCATT CCATC CCATG CCACA CCACT CCACC CCACG CCAGA CCAGT CCAGC CCAGG CCT CCTAA CCTAT CCTAC CCTAG CCTTA CCTTT CCTTC CCTTG CCTCA CCTCT CCTCC CCTCG CCTGA CCTGT CCTGC CCTGG CCC CCCAA CCCAT CCCAC CCCAG CCCTA CCCTT CCCTC CCCTG CCCCA CCCCT CCCCC CCCCG CCCGA CCCGT CCCGC CCCGG CCG CCGAA CCGAT CCGAC CCGAG CCGTA CCGTT CCGTC CCGTG CCGCA CCGCT CCGCC CCGCG CCGGA CCGGT CCGGC CCGGG CGA CGAAA CGAAT CGAAC CGAAG CGATA CGATT CGATC CGATG CGACA CGACT CGACC CGACG CGAGA CGAGT CGAGC CGAGG CGT CGTAA CGTAT CGTAC CGTAG CGTTA CGTTT CGTTC CGTTG CGTCA CGTCT CGTCC CGTCG CGTGA CGTGT CGTGC CGTGG CGC CGCAA CGCAT CGCAC CGCAG CGCTA CGCTT CGCTC CGCTG CGCCA CGCCT CGCCC CGCCG CGCGA CGCGT CGCGC CGCGG CGG CGGAA CGGAT CGGAC CGGAG CGGTA CGGTT CGGTC CGGTG CGGCA CGGCT CGGCC CGGCG CGGGA CGGGT CGGGC CGGGG GAA GAAAA GAAAT GAAAC GAAAG GAATA GAATT GAATC GAATG GAACA GAACT GAACC GAACG GAAGA GAAGT GAAGC GAAGG GAT GATAA GATAT GATAC GATAG GATTA GATTT GATTC GATTG GATCA GATCT GATCC GATCG GATGA GATGT GATGC GATGG GAC GACAA GACAT GACAC GACAG GACTA GACTT GACTC GACTG GACCA GACCT GACCC GACCG GACGA GACGT GACGC GACGG GAG GAGAA GAGAT GAGAC GAGAG GAGTA GAGTT GAGTC GAGTG GAGCA GAGCT GAGCC GAGCG GAGGA GAGGT GAGGC GAGGG GTA GTAAA GTAAT GTAAC GTAAG GTATA GTATT GTATC GTATG GTACA GTACT GTACC GTACG GTAGA GTAGT GTAGC GTAGG GTT GTTAA GTTAT GTTAC GTTAG GTTTA GTTTT GTTTC GTTTG GTTCA GTTCT GTTCC GTTCG GTTGA GTTGT GTTGC GTTGG GTC GTCAA GTCAT GTCAC GTCAG GTCTA GTCTT GTCTC GTCTG GTCCA GTCCT GTCCC GTCCG GTCGA GTCGT GTCGC GTCGG GTG GTGAA GTGAT GTGAC GTGAG GTGTA GTGTT GTGTC GTGTG GTGCA GTGCT GTGCC GTGCG GTGGA GTGGT GTGGC GTGGG GCA GCAAA GCAAT GCAAC GCAAG GCATA GCATT GCATC GCATG GCACA GCACT GCACC GCACG GCAGA GCAGT GCAGC GCAGG GCT GCTAA GCTAT GCTAC GCTAG GCTTA GCTTT GCTTC GCTTG GCTCA GCTCT GCTCC GCTCG GCTGA GCTGT GCTGC GCTGG GCC GCCAA GCCAT GCCAC GCCAG GCCTA GCCTT GCCTC GCCTG GCCCA GCCCT GCCCC GCCCG GCCGA GCCGT GCCGC GCCGG GCG GCGAA GCGAT GCGAC GCGAG GCGTA GCGTT GCGTC GCGTG GCGCA GCGCT GCGCC GCGCG GCGGA GCGGT GCGGC GCGGG GGA GGAAA GGAAT GGAAC GGAAG GGATA GGATT GGATC GGATG GGACA GGACT GGACC GGACG GGAGA GGAGT GGAGC GGAGG GGT GGTAA GGTAT GGTAC GGTAG GGTTA GGTTT GGTTC GGTTG GGTCA GGTCT GGTCC GGTCG GGTGA GGTGT GGTGC GGTGG GGC GGCAA GGCAT GGCAC GGCAG GGCTA GGCTT GGCTC GGCTG GGCCA GGCCT GGCCC GGCCG GGCGA GGCGT GGCGC GGCGG GGG GGGAA GGGAT GGGAC GGGAG GGGTA GGGTT GGGTC GGGTG GGGCA GGGCT GGGCC GGGCG GGGGA GGGGT GGGGC GGGGG

fastp --in1 Sig_18_tissue_S3_L001_R1_001.fastq.gz --in2 Sig_18_tissue_S3_L001_R2_001.fastq.gz --out1 Sig_18_tissue/Sig_18_tissue_R1.fastq.gz --out2 Sig_18_tissue/Sig_18_tissue_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json Sig_18_tissue.fastp.json --html Sig_18_tissue.fastp.html --thread 4