This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_BL.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2712.461 CPU sec, 100.069 real sec [M::process] read 125268 sequences (18081190 bp) in 0.171 CPU sec, 0.180 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 44781, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 281, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (306.22, 154.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125268 reads in 28.395 CPU sec, 0.931 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125268 overall: 67988697088 mem 2286.256 user 464.987 sys 111.415 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_BL.L008\tPU:L008\tSM:HCC1395_BL\tLB:HCC1395_BL\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_BL_R1.fastq.gz HCC1395_BL_R2.fastq.gz [main] Real time: 111.445 sec; CPU: 2751.247 sec sorting block 0, #reads = 125405 mem = 59527015 peak = 163647488 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7353993 output reads: 125405 bam_mem_sort: 1 calls 0.016 user 0.000 sys 0.026 real bam_write: 1 calls 0.090 user 0.025 sys 0.107 real execute: 1 calls 0.055 user 0.128 sys 111.869 real merge_files: 1 calls 0.041 user 0.102 sys 0.270 real parse_chunk: 2 calls 0.037 user 0.034 sys 0.080 real read_chunk: 7 calls 0.018 user 0.071 sys 10.354 real sort_block: 1 calls 0.133 user 0.105 sys 10.400 real write_chunk: 3 calls 0.009 user 0.017 sys 0.051 real overall: 458289152 mem 1.186 user 0.438 sys 111.886 real ls: cannot access '*.cram': No such file or directory