Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ed/b40e7431ef0011aec7cb5e0d0590cd/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/df/9660a5d1f6339209f51542173b898c/output/Sig_18_tissue_S3_L005_R1_001.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ed/b40e7431ef0011aec7cb5e0d0590cd/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/df/9660a5d1f6339209f51542173b898c/output/Sig_18_tissue_S3_L005_R2_001.fastq.gz
==> STAGING COMPLETE (4 inputs)

Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

Read1 before filtering:
total reads: 62305
total bases: 9345750
Q20 bases: 9184543(98.2751%)
Q30 bases: 8911359(95.352%)
Q40 bases: 8911359(95.352%)

Read2 before filtering:
total reads: 62305
total bases: 9345750
Q20 bases: 9130570(97.6976%)
Q30 bases: 8846965(94.663%)
Q40 bases: 8846965(94.663%)

Read1 after filtering:
total reads: 62305
total bases: 8814399
Q20 bases: 8712182(98.8403%)
Q30 bases: 8478417(96.1883%)
Q40 bases: 8478417(96.1883%)

Read2 after filtering:
total reads: 62305
total bases: 8815110
Q20 bases: 8655714(98.1918%)
Q30 bases: 8424209(95.5656%)
Q40 bases: 8424209(95.5656%)

Filtering result:
reads passed filter: 124610
reads failed due to low quality: 0
reads failed due to too many N: 0
reads failed due to too short: 0
reads with adapter trimmed: 29204
bases trimmed due to adapters: 1061991

Duplication rate: 0.799294%

Insert size peak (evaluated by paired-end reads): 179

JSON report: Sig_18_tissue.fastp.json
HTML report: Sig_18_tissue.fastp.html

fastp --in1 Sig_18_tissue_S3_L005_R1_001.fastq.gz --in2 Sig_18_tissue_S3_L005_R2_001.fastq.gz --out1 Sig_18_tissue/Sig_18_tissue_R1.fastq.gz --out2 Sig_18_tissue/Sig_18_tissue_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json Sig_18_tissue.fastp.json --html Sig_18_tissue.fastp.html --thread 4 
fastp v1.0.1, time used: 2 seconds