Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/dc/4bd5ce6318f8a9cb51df9fc205555d/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/dc/4bd5ce6318f8a9cb51df9fc205555d/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0d/a6cddcd6c977973443d7744a19ca98/output/Sig_18_tissue_S3_L004_R2_001.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0d/a6cddcd6c977973443d7744a19ca98/output/Sig_18_tissue_S3_L004_R1_001.fastq.gz
==> STAGING COMPLETE (4 inputs)

Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

Read1 before filtering:
total reads: 62452
total bases: 9367800
Q20 bases: 9205520(98.2677%)
Q30 bases: 8929482(95.321%)
Q40 bases: 8929482(95.321%)

Read2 before filtering:
total reads: 62452
total bases: 9367800
Q20 bases: 9151629(97.6924%)
Q30 bases: 8864270(94.6249%)
Q40 bases: 8864270(94.6249%)

Read1 after filtering:
total reads: 62451
total bases: 8836476
Q20 bases: 8733380(98.8333%)
Q30 bases: 8497408(96.1629%)
Q40 bases: 8497408(96.1629%)

Read2 after filtering:
total reads: 62451
total bases: 8836926
Q20 bases: 8676206(98.1813%)
Q30 bases: 8441021(95.5199%)
Q40 bases: 8441021(95.5199%)

Filtering result:
reads passed filter: 124902
reads failed due to low quality: 2
reads failed due to too many N: 0
reads failed due to too short: 0
reads with adapter trimmed: 29332
bases trimmed due to adapters: 1061992

Duplication rate: 0.754179%

Insert size peak (evaluated by paired-end reads): 143

JSON report: Sig_18_tissue.fastp.json
HTML report: Sig_18_tissue.fastp.html

fastp --in1 Sig_18_tissue_S3_L004_R1_001.fastq.gz --in2 Sig_18_tissue_S3_L004_R2_001.fastq.gz --out1 Sig_18_tissue/Sig_18_tissue_R1.fastq.gz --out2 Sig_18_tissue/Sig_18_tissue_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json Sig_18_tissue.fastp.json --html Sig_18_tissue.fastp.html --thread 4 
fastp v1.0.1, time used: 2 seconds