cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_tumor.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1965.779 CPU sec, 68.040 real sec [M::process] read 125124 sequences (17999868 bp) in 0.180 CPU sec, 0.183 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 43763, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 272, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (296.95, 149.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125124 reads in 17.400 CPU sec, 0.585 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125124 overall: 68403859456 mem 1848.011 user 142.584 sys 76.044 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_tumor.L007\tPU:L007\tSM:HCC1395_tumor\tLB:HCC1395_tumor\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_tumor_R1.fastq.gz HCC1395_tumor_R2.fastq.gz [main] Real time: 76.075 sec; CPU: 1990.599 sec sorting block 0, #reads = 125293 mem = 59924104 peak = 157360128 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7303558 output reads: 125293 bam_mem_sort: 1 calls 0.035 user 0.000 sys 0.042 real bam_write: 1 calls 0.063 user 0.009 sys 0.065 real execute: 1 calls 0.062 user 0.165 sys 76.544 real merge_files: 1 calls 0.042 user 0.135 sys 0.338 real parse_chunk: 2 calls 0.061 user 0.008 sys 0.083 real read_chunk: 8 calls 0.029 user 0.080 sys 7.398 real sort_block: 1 calls 0.148 user 0.059 sys 7.379 real write_chunk: 4 calls 0.012 user 0.028 sys 0.058 real overall: 403931136 mem 1.532 user 0.418 sys 76.563 real ls: cannot access '*.cram': No such file or directory