This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_Blood.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2555.275 CPU sec, 94.051 real sec [M::process] read 125346 sequences (18175258 bp) in 0.170 CPU sec, 0.176 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 45082, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 292, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (316.67, 156.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125346 reads in 28.930 CPU sec, 0.956 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125346 overall: 68082991104 mem 2130.486 user 464.326 sys 105.610 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_Blood.L004\tPU:L004\tSM:Sig_18_Blood\tLB:Sig_18_Blood\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_Blood_R1.fastq.gz Sig_18_Blood_R2.fastq.gz [main] Real time: 105.641 sec; CPU: 2594.815 sec sorting block 0, #reads = 125458 mem = 60226619 peak = 163676160 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7445623 output reads: 125458 bam_mem_sort: 1 calls 0.022 user 0.000 sys 0.027 real bam_write: 1 calls 0.111 user 0.000 sys 0.113 real execute: 1 calls 0.074 user 0.121 sys 106.083 real merge_files: 1 calls 0.043 user 0.111 sys 0.283 real parse_chunk: 2 calls 0.047 user 0.032 sys 0.092 real read_chunk: 8 calls 0.005 user 0.069 sys 10.550 real sort_block: 1 calls 0.165 user 0.072 sys 10.599 real write_chunk: 4 calls 0.007 user 0.021 sys 0.051 real overall: 457113600 mem 1.240 user 0.440 sys 106.100 real ls: cannot access '*.cram': No such file or directory