Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b8/388a8a62a81978a49d551ad5c61f27/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b8/388a8a62a81978a49d551ad5c61f27/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/08/bc229ddd360b5814901539299f56ea/output/Sig_18_tissue_S3_L003_R2_001.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/08/bc229ddd360b5814901539299f56ea/output/Sig_18_tissue_S3_L003_R1_001.fastq.gz
==> STAGING COMPLETE (4 inputs)

Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

Read1 before filtering:
total reads: 62899
total bases: 9434850
Q20 bases: 9272103(98.275%)
Q30 bases: 8995714(95.3456%)
Q40 bases: 8995714(95.3456%)

Read2 before filtering:
total reads: 62899
total bases: 9434850
Q20 bases: 9217984(97.7014%)
Q30 bases: 8930666(94.6562%)
Q40 bases: 8930666(94.6562%)

Read1 after filtering:
total reads: 62896
total bases: 8903870
Q20 bases: 8799424(98.827%)
Q30 bases: 8562137(96.162%)
Q40 bases: 8562137(96.162%)

Read2 after filtering:
total reads: 62896
total bases: 8904361
Q20 bases: 8743682(98.1955%)
Q30 bases: 8508716(95.5567%)
Q40 bases: 8508716(95.5567%)

Filtering result:
reads passed filter: 125792
reads failed due to low quality: 6
reads failed due to too many N: 0
reads failed due to too short: 0
reads with adapter trimmed: 29407
bases trimmed due to adapters: 1060725

Duplication rate: 0.785386%

Insert size peak (evaluated by paired-end reads): 180

JSON report: Sig_18_tissue.fastp.json
HTML report: Sig_18_tissue.fastp.html

fastp --in1 Sig_18_tissue_S3_L003_R1_001.fastq.gz --in2 Sig_18_tissue_S3_L003_R2_001.fastq.gz --out1 Sig_18_tissue/Sig_18_tissue_R1.fastq.gz --out2 Sig_18_tissue/Sig_18_tissue_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json Sig_18_tissue.fastp.json --html Sig_18_tissue.fastp.html --thread 4 
fastp v1.0.1, time used: 1 seconds