Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/08/bc229ddd360b5814901539299f56ea/output/HCC1395_BL_S2_L003_R2_001.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d0/b0120c2ec844ac347e1a181f05e7ab/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d0/b0120c2ec844ac347e1a181f05e7ab/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/08/bc229ddd360b5814901539299f56ea/output/HCC1395_BL_S2_L003_R1_001.fastq.gz ==> STAGING COMPLETE (4 inputs) Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 62275 total bases: 9341250 Q20 bases: 9173994(98.2095%) Q30 bases: 8847351(94.7127%) Q40 bases: 8847351(94.7127%) Read2 before filtering: total reads: 62275 total bases: 9341250 Q20 bases: 9114858(97.5764%) Q30 bases: 8716485(93.3118%) Q40 bases: 8716485(93.3118%) Read1 after filtering: total reads: 62274 total bases: 8991629 Q20 bases: 8859478(98.5303%) Q30 bases: 8563509(95.2387%) Q40 bases: 8563509(95.2387%) Read2 after filtering: total reads: 62274 total bases: 8991886 Q20 bases: 8797983(97.8436%) Q30 bases: 8436445(93.8229%) Q40 bases: 8436445(93.8229%) Filtering result: reads passed filter: 124548 reads failed due to low quality: 2 reads failed due to too many N: 0 reads failed due to too short: 0 reads with adapter trimmed: 19099 bases trimmed due to adapters: 698687 Duplication rate: 0.892814% Insert size peak (evaluated by paired-end reads): 170 JSON report: HCC1395_BL.fastp.json HTML report: HCC1395_BL.fastp.html fastp --in1 HCC1395_BL_S2_L003_R1_001.fastq.gz --in2 HCC1395_BL_S2_L003_R2_001.fastq.gz --out1 HCC1395_BL/HCC1395_BL_R1.fastq.gz --out2 HCC1395_BL/HCC1395_BL_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json HCC1395_BL.fastp.json --html HCC1395_BL.fastp.html --thread 4 fastp v1.0.1, time used: 2 seconds