FastQCFastQC Report
Sat 16 May 2026
NTC_0001_0001_23KMKGLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_23KMKGLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096639
Total Bases467.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC72

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTGGG83838827.07412778822459TruSeq Adapter, Index 9 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTGGT71434323.068333118584373TruSeq Adapter, Index 9 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTAGT43393014.012934668845803TruSeq Adapter, Index 9 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTAGG2971049.594402188953895TruSeq Adapter, Index 9 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTGG244300.7889198579492153TruSeq Adapter, Index 9 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTGGTGTG176450.5698113341593902TruSeq Adapter, Index 9 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTGGGGTG164730.5319638485467632TruSeq Adapter, Index 9 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTGGGGGG151020.48769004071834005TruSeq Adapter, Index 9 (97% over 36bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA112910.36462112632437943No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTAG100780.3254496245768396TruSeq Adapter, Index 9 (97% over 39bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC87480.2824998328833293No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTGGTATG81620.26357609007701577TruSeq Adapter, Index 9 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTAGTGTG72470.23402792511493914TruSeq Adapter, Index 9 (97% over 36bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCAGATCGGAAGAGCA68170.22014190223658617No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTAGTATG67870.2191731099427476TruSeq Adapter, Index 9 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTGGGG66770.21562087153200615TruSeq Adapter, Index 9 (97% over 38bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAG66060.21332806310325486No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT49270.1591079877247558No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTAGGGTG48820.1576547992839979TruSeq Adapter, Index 9 (97% over 36bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG47720.15410256087325644No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCAGATCGGAAGAGCAC39940.12897854738637599No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCAGATCGGAAGAGCA38190.12332725900565096No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTGGTG38000.12271369055288654TruSeq Adapter, Index 9 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTGGGATG32780.10585670464009528TruSeq Adapter, Index 9 (97% over 36bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCACAGATCGGAAGAGC32260.1041774646641084No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGAGATCGGAAGAGCACACGTC31890.10298262083504083No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCGGGG31690.10233675930581511TruSeq Adapter, Index 9 (97% over 39bp)
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAGAGATCGGAAGAGCACACGTC31660.10223988007643126No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)