FastQCFastQC Report
Sat 16 May 2026
NTC_0001_0001_23KMKGLT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_23KMKGLT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502222
Total Bases22 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA111142.2129655809582216No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC87151.7352883784461852No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC68861.3711068013746908No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC66401.322124478816141No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT48820.9720800761416267No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG46760.9310623588771499No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC40560.8076109768190163No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC38620.7689826411427615No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG33050.658075512422793No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC32420.6455312590846279No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAG31870.6345799268052774No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC28500.5674781272027111No Hit
ACGGGAAGGCCCGGTGGGGAA25130.5003763276001449No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC21840.43486744905639335No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC18520.36876122511558634No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG18430.3669691889244199No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC17870.3558187415127175No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC17360.34566386976277425No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC15050.29966827418950187No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG15030.29927004392479817No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG13890.27657091883668977No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG13430.2674116227485056No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC12910.25705763586621055No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC12780.2544691391456368No Hit
CACGGGAAGGCCCGGTGGGGAA12480.24849568517508192No Hit
AACCGAAACCGAAACCTA11370.226393905484029No Hit
TAGGTTTCGGTTTCGGTT11320.22539832982226984No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG10530.20966823436647536No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC10360.20628327711649427No Hit
GCCCCCCCGGCAGCAGCAC10270.20449124092532783No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC9600.19115052705775534No Hit
GGGGGAGAGAAAGAGAAAGAAGGGCG8960.1784071585872383No Hit
GGCCCCCCCGGCAGCAGCAC8940.17800892832253465No Hit
GGAGGAACGGGGGGCGCGAAG8800.17522131646960906No Hit
CGCGGGGGAGAGAAAGAGAAAGAAGGGCG8440.16805317170494322No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC8310.16546467498436945No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC8280.16486732958731398No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC8100.16128325720498105No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG8060.16048679667557375No Hit
GGGGAGAGAGAGAGAGAGAGGGCGCGAAG8020.15969033614616643No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG7960.15849564535205546No Hit
CGGGGAAGGCCCGGTGGGGAA7870.15670360916088902No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC7730.1539159973079634No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC7640.15212396111679696No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC7530.14993369466092685No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC7440.14814165846976038No Hit
GGACGCGGGGCCGGGGGGCGG7380.1469469676756494No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC7330.14595139201389026No Hit
GGGGGAAGGCCCGGTGGGGAA7010.13957970777863177No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC6930.13798678671981712No Hit
GGACGCGGGGCCGGGGGGCCC6900.13738944132276165No Hit
CCGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC6900.13738944132276165No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC6770.1348009446021879No Hit
TTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT6550.13042041169044766No Hit
GGCCCCCCCGGCAGCAGC6470.128827490631633No Hit
TGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC6380.12703545444046657No Hit
CCGGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC6310.12564164851400378No Hit
GCTGCTGCCGGGGGGGCC6290.1252434182493001No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC6280.12504430311694828No Hit
GCTGCTGCCGGGGGGCCC6220.12384961232283732No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGG6180.12305315179343No Hit
GGGGGATTCCCCGCGGGGGTGGG6120.12185846099931903No Hit
CCACCCCCGCGGGGAATCCCCC6080.12106200046991171No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAG5980.11907084914639342No Hit
GGACGCGGGGCCGGGGGGCGGG5780.11508854649935685No Hit
CCCACCCCCGCGGGGAATCCCCC5720.11389385570524588No Hit
GCCCCGGGGGCGGACCCGAAAGATGG5600.11150447411702395No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGG5590.11130535898467211No Hit
CGCGCCCTCTCTCTCTCTCTCTCCCCCGC5590.11130535898467211No Hit
GGACGCGGGGCCGGGGGGC5580.11110624385232029No Hit
GCCCCCCCGGCAGCAGC5560.11070801358761663No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC5460.10871686226409834No Hit
CTGCCGGGGGGGCTGTAACACTCGGGGGGCCC5380.10712394120528372No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCCGC5290.10533190501411727No Hit
CGGGGGAGAGAGAGAGAGAGAGGGCGCGAAG5230.1041372142200063No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC5150.10254429316119167No Hit
CGGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC5150.10254429316119167No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACGATCTGTATGATCTGGGG5030.10015491157296973TruSeq Adapter, Index 9 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)