FastQCFastQC Report
Sat 16 May 2026
NTC_0001_0001_23KMKGLT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_23KMKGLT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502222
Total Bases23.2 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151013.0068376136449615No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC128082.550266615162219No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA112822.246416923193329No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC67671.3474121006248234No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC66631.326704126860233No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC39270.7819251247456305No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC38160.7598233450545775No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG35270.702279071804899No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC32420.6455312590846279No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC32380.6447347985552205No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC31530.6278100123053152No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC28550.5684737028644703No Hit
TTCCCCACCGGGCCTTCCCGT24970.4971904854825157No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG21460.4273010740270239No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC18410.36657095865971623No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC17950.3574116625715321No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC17720.35283201452744006No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG16900.33650457367459014No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC14960.2978762379983354No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC14900.2966815472042244No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC13510.26900454380732025No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG13040.2596461325867843No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG12720.2532744483515258No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC12700.2528762180868222No Hit
TTCCCCACCGGGCCTTCCCGTG12400.24690276411626733No Hit
AACCGAAACCGAAACCTA11110.22121691204288144No Hit
TAGGTTTCGGTTTCGGTT11070.2204204515134741No Hit
GTGCTGCTGCCGGGGGGGC10210.20329655013121686No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC10120.20150451394005042No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC10040.19991159288123578No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG9320.18557530335190414No Hit
CTTCGCGCCCCCCGTTCCTCC8830.17581866186666453No Hit
GTGCTGCTGCCGGGGGGGCC8740.1740266256754981No Hit
CGCCCTTCTTTCTCTTTCTCTCCCCC8730.17382751054314627No Hit
CGCCCTTCTTTCTCTTTCTCTCCCCCGCG8330.16586290524907313No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG8160.16247794799909204No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAG8110.1614823723373329No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG8030.15988945127851825No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG7990.15909299074911093No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCC7860.15650449402853717No Hit
TTCCCCACCGGGCCTTCCCCG7810.15550891836677805No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC7680.15292042164620426No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC7560.15053104005798235No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG7550.1503319249256305No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC7440.14814165846976038No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC7350.14634962227859394No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG7320.14575227688153844No Hit
CCGCCCCCCGGCCCCGCGTCC7160.14256643476390918No Hit
TTCCCCACCGGGCCTTCCCCC7020.1397788229109836No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCGG6960.13858413211687262No Hit
GGGCCCCCCGGCCCCGCGTCC6880.13699121105805798No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC6830.13599563539629886No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC6730.13400448407278057No Hit
GCTGCTGCCGGGGGGGCC6340.12623899391105925No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCCGG6280.12504430311694828No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGG6270.12484518798459646No Hit
GGCCCCCCCGGCAGCAGC6150.1224558063963745No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCA6130.12205757613167084No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC6020.11986730967580074No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGC6010.11966819454344892No Hit
GGGCCCCCCGGCAGCAGC6000.11946907941109708No Hit
GGGGGATTCCCCGCGGGGGTGG5940.11827438861698612No Hit
CCCACCCCCGCGGGGAATCCCCC5940.11827438861698612No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAG5890.11727881295522696No Hit
GGGGGATTCCCCGCGGGGGTGGG5660.11269916491113492No Hit
CCCGCCCCCCGGCCCCGCGTCC5630.11210181951407942No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGCG5580.11110624385232029No Hit
CCATCTTTCGGGTCCGCCCCCGGGGC5530.11011066819056116No Hit
GCTGCTGCCGGGGGGGC5480.109115092528802No Hit
GCCCCCCGGCCCCGCGTCC5430.10811951686704287No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC5350.10652659580822822No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAG5290.10533190501411727No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAG5260.10473455961706177No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC5180.10314163855824714No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCCG5150.10254429316119167No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCCG5110.10174783263178434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)