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tih_rna_sample_00156_23KMKGLT4_1.fastp.log
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s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0001/B23KMKGLT4/nfcore_rnafusion__2cae617--20260515-145204/fastp/tih_rna_sample_00156_23KMKGLT4_1.fastp.log
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Workflow Run
dev_rna_exp0001R_2cae617
Published
May 16, 2026 1:16 AM

Content

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Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

Read1 before filtering:
total reads: 28063786
total bases: 4237631686
Q20 bases: 4037029156(95.2662%)
Q30 bases: 3755266314(88.6171%)

Read2 before filtering:
total reads: 28063786
total bases: 4237631686
Q20 bases: 4083872879(96.3716%)
Q30 bases: 3792827277(89.5035%)

Read1 after filtering:
total reads: 25494091
total bases: 2814256062
Q20 bases: 2799067871(99.4603%)
Q30 bases: 2738246935(97.2991%)

Read2 after filtering:
total reads: 25494091
total bases: 2831417826
Q20 bases: 2794599971(98.6997%)
Q30 bases: 2706099776(95.574%)

Filtering result:
reads passed filter: 50988182
reads failed due to low quality: 1342204
reads failed due to too many N: 7542
reads failed due to too short: 3789644
reads with adapter trimmed: 42950318
bases trimmed due to adapters: 2155204511

Duplication rate: 42.9419%

Insert size peak (evaluated by paired-end reads): 107

JSON report: tih_rna_sample_00156_23KMKGLT4_1.fastp.json
HTML report: tih_rna_sample_00156_23KMKGLT4_1.fastp.html

fastp --in1 tih_rna_sample_00156_23KMKGLT4_1_1.fastq.gz --in2 tih_rna_sample_00156_23KMKGLT4_1_2.fastq.gz --out1 tih_rna_sample_00156_23KMKGLT4_1_1.fastp.fastq.gz --out2 tih_rna_sample_00156_23KMKGLT4_1_2.fastp.fastq.gz --json tih_rna_sample_00156_23KMKGLT4_1.fastp.json --html tih_rna_sample_00156_23KMKGLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 
fastp v0.23.4, time used: 45 seconds