FastQCFastQC Report
Sat 16 May 2026
tih_rna_sample_00156_23KMKGLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametih_rna_sample_00156_23KMKGLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28063786
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCAACACAATCTGGG10961293.9058486264112764TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCAACACAATCTAGG6137242.186889537997475TruSeq Adapter, Index 15 (97% over 36bp)
GCGGTTATCAGTACGACCTGGCATGAAAACTATTCCTTCCTGTGGATTTT1296740.46206880283365903No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAAGCACTTTA1054850.3758758707752404No Hit
GCGGCATTTAACCATTACGCCAGCATCCTTGACAAAAGTCGCAATCCTCA1014590.36152998030985556No Hit
GTCGTATACAAATGATTTATCCTTGCGCAAAATGACATTGCAATTCGCCG965940.34419447183640867No Hit
CTCGTCGAAAGCACTTTACAAATAACTGGGATCCTCGCCACACGGGATTC643690.22936677182472812No Hit
CACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAAGCACTT614990.21914006898427746No Hit
GCTGCGGTTATCAGTACGACCTGGCATGAAAACTATTCCTTCCTGTGGAT603180.2149317985819875No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC542140.19318134766278505No Hit
CCCGGATCAGCCCGTATAGGACTTTGAATGCTAGAACGTGGAAAATGAAT535730.19089726525138126No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACGCAACACAATCTGGGGGG516720.184123410861243TruSeq Adapter, Index 15 (96% over 33bp)
GTGAAGAATCCATATCCAGGTTCCGGAATATTAACCGGATTCCCTTTCGA508750.18128345191913878No Hit
GTCGCAATCCTCAGTCCCAGCTGGCTGTATTCCCACGGGCTATAACACTC501370.17865372833159432No Hit
CCCTGTTGTACATTTAAGTCGTATACAAATGATTTATCCTTGCGCAAAAT446930.15925506273458614No Hit
CTCGACTCGTCGAAAGCACTTTACAAATAACTGGGATCCTCGCCACACGG444430.1583642349610277No Hit
ACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAAGCACTTT437290.15582003083974486No Hit
GGGGCTCCGGGGGCGGGGAGCGGGGCGTGGGCGGGAGGAGGGGAGGAGGC421180.15007953666693438No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT415470.14804488603212695No Hit
CTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCA413480.14733578712437445No Hit
GCCCACTAAAAGCTCTTCATTCAAATGTCCACGTTCAATTAAGCAACAAG399940.14251106390278206No Hit
GTCGCCTTCTTCAAATTACAACTCGGACGTCGAAGACGCCAGATTTCAAA392830.13997754971478188No Hit
GCGGGGCTCCGGGGGCGGGGAGCGGGGCGTGGGCGGGAGGAGGGGAGGAG390710.13922212776280435No Hit
GTCCGCGGGGCTCCGGGGGCGGGGAGCGGGGCGTGGGCGGGAGGAGGGGA390010.138972695986208No Hit
GCCTTCTTCAAATTACAACTCGGACGTCGAAGACGCCAGATTTCAAATTT387430.1380533617238957No Hit
GGGGCGGGGAGCGGGGCGTGGGCGGGAGGAGGGGAGGAGGCGTGGGGGGG370220.13192090333071954No Hit
GCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAGAATCCATATCCA369660.13172135790944242No Hit
CGTATACAAATGATTTATCCTTGCGCAAAATGACATTGCAATTCGCCGGC368340.13125100084500357No Hit
GGCCCACTAAAAGCTCTTCATTCAAATGTCCACGTTCAATTAAGCAACAA364420.12985418289606399No Hit
GCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTC356310.12696433759864048No Hit
GCGCAAAATGACATTGCAATTCGCCGGCAAGCACCCAAAGCCTTTCCGCT352410.12557464627188933No Hit
GGCGGCATTTAACCATTACGCCAGCATCCTTGACAAAAGTCGCAATCCTC342160.1219222524002998No Hit
CGGGGCTCCGGGGGCGGGGAGCGGGGCGTGGGCGGGAGGAGGGGAGGAGG340450.12131292620318586No Hit
GCCCACTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAAGCACTTT335880.11968449303312106No Hit
CCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA332290.11840526435029114No Hit
CCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGT330270.11768547550925594No Hit
CTGCGGTTATCAGTACGACCTGGCATGAAAACTATTCCTTCCTGTGGATT322260.11483126332277477No Hit
GCTGCATTCCCAAACAACTCGACTCGTCGAAAGCACTTTACAAATAACTG313080.11156014373826825No Hit
CCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAAGCACT308780.11002791996774777No Hit
ATACAAATGATTTATCCTTGCGCAAAATGACATTGCAATTCGCCGGCAAG303170.10802890244388265No Hit
CCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGAGTTAA301610.1074730259131822No Hit
GCTACTACCACCAAGATCTGCACTAGAGGCCGTTCTACCCAGCTTTACAG298340.10630782318536779No Hit
GAAAACTATTCCTTCCTGTGGATTTTCAAGGGCCGTCGCGGGCGCACCGG297300.10593723883156748No Hit
GAAGAATCCATATCCAGGTTCCGGAATATTAACCGGATTCCCTTTCGATG296230.10555596454448449No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)