Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 98313547 total bases: 14845345597 Q20 bases: 14367896282(96.7838%) Q30 bases: 13682293156(92.1655%) Read2 before filtering: total reads: 98313547 total bases: 14845345597 Q20 bases: 14468561005(97.4619%) Q30 bases: 13743642452(92.5788%) Read1 after filtering: total reads: 95737431 total bases: 10881004754 Q20 bases: 10831058103(99.541%) Q30 bases: 10606301366(97.4754%) Read2 after filtering: total reads: 95737431 total bases: 10898392797 Q20 bases: 10811783500(99.2053%) Q30 bases: 10534288159(96.6591%) Filtering result: reads passed filter: 191474862 reads failed due to low quality: 3056544 reads failed due to too many N: 30118 reads failed due to too short: 2065570 reads with adapter trimmed: 156186373 bases trimmed due to adapters: 7240583246 Duplication rate: 44.63% Insert size peak (evaluated by paired-end reads): 109 JSON report: tih_rna_sample_00482_23KMKGLT4_1.fastp.json HTML report: tih_rna_sample_00482_23KMKGLT4_1.fastp.html fastp --in1 tih_rna_sample_00482_23KMKGLT4_1_1.fastq.gz --in2 tih_rna_sample_00482_23KMKGLT4_1_2.fastq.gz --out1 tih_rna_sample_00482_23KMKGLT4_1_1.fastp.fastq.gz --out2 tih_rna_sample_00482_23KMKGLT4_1_2.fastp.fastq.gz --json tih_rna_sample_00482_23KMKGLT4_1.fastp.json --html tih_rna_sample_00482_23KMKGLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 237 seconds