Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 113617216 total bases: 17156199616 Q20 bases: 16656144463(97.0853%) Q30 bases: 15888378253(92.6101%) Read2 before filtering: total reads: 113617216 total bases: 17156199616 Q20 bases: 16745717309(97.6074%) Q30 bases: 15927230483(92.8366%) Read1 after filtering: total reads: 110759071 total bases: 12565976984 Q20 bases: 12510793751(99.5609%) Q30 bases: 12255973456(97.533%) Read2 after filtering: total reads: 110759071 total bases: 12586455517 Q20 bases: 12486284571(99.2041%) Q30 bases: 12163080016(96.6363%) Filtering result: reads passed filter: 221518142 reads failed due to low quality: 3423260 reads failed due to too many N: 34576 reads failed due to too short: 2258454 reads with adapter trimmed: 179445209 bases trimmed due to adapters: 8418674506 Duplication rate: 45.7891% Insert size peak (evaluated by paired-end reads): 97 JSON report: tih_rna_sample_00338_23KMKGLT4_1.fastp.json HTML report: tih_rna_sample_00338_23KMKGLT4_1.fastp.html fastp --in1 tih_rna_sample_00338_23KMKGLT4_1_1.fastq.gz --in2 tih_rna_sample_00338_23KMKGLT4_1_2.fastq.gz --out1 tih_rna_sample_00338_23KMKGLT4_1_1.fastp.fastq.gz --out2 tih_rna_sample_00338_23KMKGLT4_1_2.fastp.fastq.gz --json tih_rna_sample_00338_23KMKGLT4_1.fastp.json --html tih_rna_sample_00338_23KMKGLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 275 seconds