Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 148051492 total bases: 22355775292 Q20 bases: 21755570006(97.3152%) Q30 bases: 20692665904(92.5607%) Read2 before filtering: total reads: 148051492 total bases: 22355775292 Q20 bases: 21641912912(96.8068%) Q30 bases: 20399829137(91.2508%) Read1 after filtering: total reads: 144221899 total bases: 16264906058 Q20 bases: 16174742160(99.4457%) Q30 bases: 15813837735(97.2267%) Read2 after filtering: total reads: 144221899 total bases: 16337361200 Q20 bases: 16124150846(98.695%) Q30 bases: 15582122293(95.3772%) Filtering result: reads passed filter: 288443798 reads failed due to low quality: 6278102 reads failed due to too many N: 44710 reads failed due to too short: 1336374 reads with adapter trimmed: 236186368 bases trimmed due to adapters: 11123765337 Duplication rate: 51.7132% Insert size peak (evaluated by paired-end reads): 106 JSON report: tih_rna_sample_00300_23KMKGLT4_1.fastp.json HTML report: tih_rna_sample_00300_23KMKGLT4_1.fastp.html fastp --in1 tih_rna_sample_00300_23KMKGLT4_1_1.fastq.gz --in2 tih_rna_sample_00300_23KMKGLT4_1_2.fastq.gz --out1 tih_rna_sample_00300_23KMKGLT4_1_1.fastp.fastq.gz --out2 tih_rna_sample_00300_23KMKGLT4_1_2.fastp.fastq.gz --json tih_rna_sample_00300_23KMKGLT4_1.fastp.json --html tih_rna_sample_00300_23KMKGLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 340 seconds