File Info

Filename
qc_report_detailed.txt
Full Path
s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0001exp0004/B23KMKGLT4/nfcore_rnafusion__73d51683--20260607-225254/samplesheet_check/qc_report_detailed.txt
Size
18.0 KB
Published
Jun 07, 2026 11:44 PM
================================================================================
BCL CONVERT QC REPORT - DETAILED
================================================================================

1. % UNDETERMINED PER LANE
--------------------------------------------------------------------------------
      %_Undetermined  Undetermined_Reads  Total_Reads
Lane                                                 
1               5.52           221059769   4004297187
2               5.90           225513465   3822265332
3               5.35           200487374   3746919997
4               5.32           200329565   3768247701
5               4.74           171826374   3628757465
6               4.75           184145712   3874163237
7               4.94           190151678   3850667784
8               5.09           202217613   3974555505

2. SAMPLES WITH LOW READS (NON-NTC)
--------------------------------------------------------------------------------
No samples found with low reads

3. NTC SAMPLES WITH HIGH READS
--------------------------------------------------------------------------------
Found 1 NTC samples with high reads:
                    SampleID                  Index  # Reads
0  NTC_0001_0001_23KMKGLT4_1  GATCTGTATG-ATCGAAGAGC  3096639

4. TOP UNKNOWN BARCODES
--------------------------------------------------------------------------------
Found 132 significant unknown barcodes:
     Lane       index      index2   # Reads  % of Unknown Barcodes  % of All Reads  Percentage_of_Lane  Percentage_of_Total
0       1  CTCACCAGTT  GGGGGGGGGG   8458630               0.038264        0.002112            0.211239             0.027580
1       1  TAGAAGATCG  GGGGGGGGGG   7394299               0.033449        0.001847            0.184659             0.024109
2       1  AGTCCAGCGT  GGGGGGGGGG   7306092               0.033050        0.001825            0.182456             0.023822
3       1  AATAGACTGC  GGGGGGGGGG   5668678               0.025643        0.001416            0.141565             0.018483
4       1  AGGACGGACA  GGGGGGGGGG   4620766               0.020903        0.001154            0.115395             0.015066
5       1  GCGAAGTAGG  CAGGCCATGT   4572171               0.020683        0.001142            0.114182             0.014908
6       1  CATCACCTAA  GGGGGGGGGG   4463209               0.020190        0.001115            0.111460             0.014552
7       1  ACGGTCGCAT  CGTCATCGAA   4368466               0.019761        0.001091            0.109094             0.014244
8       1  CCTGGAATTA  GGGGGGGGGG   3811715               0.017243        0.000952            0.095191             0.012428
9       1  CGGCATATAC  GGGGGGGGGG   3713173               0.016797        0.000927            0.092730             0.012107
10      1  GCGACTCGTA  ACCATTGTGT   3307477               0.014962        0.000826            0.082598             0.010784
11      1  GCGACTCGTA  GGGGGGGGGG   3287995               0.014874        0.000821            0.082112             0.010721
12      1  CAACGTTGAC  GGGGGGGGGG   3189964               0.014430        0.000797            0.079664             0.010401
13      1  ATGATCAACG  GGGGGGGGGG   3151810               0.014258        0.000787            0.078711             0.010277
14      1  AGGCAGCCAT  GGGGGGGGGG   3149692               0.014248        0.000787            0.078658             0.010270
15      1  TGTTACAGAG  CGTCATCGAA   2979753               0.013479        0.000744            0.074414             0.009716
16      1  ACGGTCGCAT  AAGCTATAGC   2870719               0.012986        0.000717            0.071691             0.009360
17      1  GCGCGGTTAA  GGGGGGGGGG   2790916               0.012625        0.000697            0.069698             0.009100
18      1  TTCAGATCCA  GGGGGGGGGG   2789274               0.012618        0.000697            0.069657             0.009095
19      1  GCGCGGTTAA  GTACTCTCTA   2722580               0.012316        0.000680            0.067991             0.008877
20      1  CCTATGTGTA  GGGGGGGGGG   2607642               0.011796        0.000651            0.065121             0.008502
21      1  CCTGGAATTA  TGTGTACCAG   2290216               0.010360        0.000572            0.057194             0.007467
22      1  CCTGGAATTA  GTACTCTCTA   2016250               0.009121        0.000504            0.050352             0.006574
23      2  CTCACCAGTT  GGGGGGGGGG   6725259               0.029822        0.001759            0.175950             0.021928
24      2  AGTCCAGCGT  GGGGGGGGGG   5750923               0.025501        0.001505            0.150458             0.018751
25      2  TAGAAGATCG  GGGGGGGGGG   5489139               0.024341        0.001436            0.143610             0.017897
26      2  AATAGACTGC  GGGGGGGGGG   4332307               0.019211        0.001133            0.113344             0.014126
27      2  GCGAAGTAGG  CAGGCCATGT   4076991               0.018079        0.001067            0.106664             0.013293
28      2  ACGGTCGCAT  CGTCATCGAA   3904348               0.017313        0.001021            0.102147             0.012730
29      2  CATCACCTAA  GGGGGGGGGG   3517635               0.015598        0.000920            0.092030             0.011469
30      2  AGGACGGACA  GGGGGGGGGG   3494155               0.015494        0.000914            0.091416             0.011393
31      2  CCTGGAATTA  GGGGGGGGGG   3012663               0.013359        0.000788            0.078819             0.009823
32      2  CGGCATATAC  GGGGGGGGGG   2945794               0.013063        0.000771            0.077069             0.009605
33      2  GCGACTCGTA  ACCATTGTGT   2944708               0.013058        0.000770            0.077041             0.009601
34      2  TGTTACAGAG  CGTCATCGAA   2654226               0.011770        0.000694            0.069441             0.008654
35      2  ACGGTCGCAT  AAGCTATAGC   2577659               0.011430        0.000674            0.067438             0.008405
36      2  CAACGTTGAC  GGGGGGGGGG   2488362               0.011034        0.000651            0.065102             0.008113
37      2  AGGCAGCCAT  GGGGGGGGGG   2463579               0.010924        0.000645            0.064453             0.008033
38      2  GCGACTCGTA  GGGGGGGGGG   2438677               0.010814        0.000638            0.063802             0.007951
39      2  GCGCGGTTAA  GTACTCTCTA   2432896               0.010788        0.000637            0.063651             0.007933
40      2  ATGATCAACG  GGGGGGGGGG   2432085               0.010785        0.000636            0.063629             0.007930
41      2  TTCAGATCCA  GGGGGGGGGG   2168461               0.009616        0.000567            0.056732             0.007070
42      2  GCGCGGTTAA  GGGGGGGGGG   2101575               0.009319        0.000550            0.054982             0.006852
43      2  CCTATGTGTA  GGGGGGGGGG   2076137               0.009206        0.000543            0.054317             0.006769
44      2  CCTGGAATTA  TGTGTACCAG   2056470               0.009119        0.000538            0.053802             0.006705
45      3  CTCACCAGTT  GGGGGGGGGG   6353099               0.031688        0.001696            0.169555             0.020714
46      3  AGTCCAGCGT  GGGGGGGGGG   5195636               0.025915        0.001387            0.138664             0.016941
47      3  TAGAAGATCG  GGGGGGGGGG   4880808               0.024345        0.001303            0.130262             0.015914
48      3  GCGAAGTAGG  CAGGCCATGT   4151455               0.020707        0.001108            0.110796             0.013536
49      3  AATAGACTGC  GGGGGGGGGG   4031237               0.020107        0.001076            0.107588             0.013144
50      3  ACGGTCGCAT  CGTCATCGAA   3987944               0.019891        0.001064            0.106433             0.013003
51      3  CATCACCTAA  GGGGGGGGGG   3349193               0.016705        0.000894            0.089385             0.010920
52      3  AGGACGGACA  GGGGGGGGGG   3196524               0.015944        0.000853            0.085311             0.010422
53      3  GCGACTCGTA  ACCATTGTGT   3006908               0.014998        0.000803            0.080250             0.009804
54      3  CGGCATATAC  GGGGGGGGGG   2881372               0.014372        0.000769            0.076900             0.009395
55      3  CCTGGAATTA  GGGGGGGGGG   2827073               0.014101        0.000755            0.075451             0.009218
56      3  TGTTACAGAG  CGTCATCGAA   2704405               0.013489        0.000722            0.072177             0.008818
57      3  ACGGTCGCAT  AAGCTATAGC   2627111               0.013104        0.000701            0.070114             0.008566
58      3  GCGCGGTTAA  GTACTCTCTA   2496230               0.012451        0.000666            0.066621             0.008139
59      3  AGGCAGCCAT  GGGGGGGGGG   2457459               0.012257        0.000656            0.065586             0.008013
60      3  CAACGTTGAC  GGGGGGGGGG   2412657               0.012034        0.000644            0.064390             0.007867
61      3  ATGATCAACG  GGGGGGGGGG   2345782               0.011700        0.000626            0.062606             0.007648
62      3  GCGACTCGTA  GGGGGGGGGG   2318844               0.011566        0.000619            0.061887             0.007561
63      3  CCTATGTGTA  GGGGGGGGGG   2111070               0.010530        0.000563            0.056341             0.006883
64      3  TTCAGATCCA  GGGGGGGGGG   2107300               0.010511        0.000562            0.056241             0.006871
65      3  CCTGGAATTA  TGTGTACCAG   2081886               0.010384        0.000556            0.055563             0.006788
66      3  GCGCGGTTAA  GGGGGGGGGG   2033531               0.010143        0.000543            0.054272             0.006630
67      4  CTCACCAGTT  GGGGGGGGGG   6049099               0.030196        0.001605            0.160528             0.019723
68      4  AGTCCAGCGT  GGGGGGGGGG   4946219               0.024690        0.001313            0.131260             0.016127
69      4  TAGAAGATCG  GGGGGGGGGG   4612384               0.023024        0.001224            0.122401             0.015039
70      4  GCGAAGTAGG  CAGGCCATGT   4281644               0.021373        0.001136            0.113624             0.013960
71      4  ACGGTCGCAT  CGTCATCGAA   4068746               0.020310        0.001080            0.107974             0.013266
72      4  AATAGACTGC  GGGGGGGGGG   3800972               0.018974        0.001009            0.100868             0.012393
73      4  CATCACCTAA  GGGGGGGGGG   3251052               0.016229        0.000863            0.086275             0.010600
74      4  GCGACTCGTA  ACCATTGTGT   3071285               0.015331        0.000815            0.081504             0.010014
75      4  AGGACGGACA  GGGGGGGGGG   3021813               0.015084        0.000802            0.080191             0.009853
76      4  CGGCATATAC  GGGGGGGGGG   2816660               0.014060        0.000747            0.074747             0.009184
77      4  TGTTACAGAG  CGTCATCGAA   2757669               0.013766        0.000732            0.073182             0.008991
78      4  CCTGGAATTA  GGGGGGGGGG   2725624               0.013606        0.000723            0.072331             0.008887
79      4  ACGGTCGCAT  AAGCTATAGC   2687520               0.013415        0.000713            0.071320             0.008763
80      4  GCGCGGTTAA  GTACTCTCTA   2562849               0.012793        0.000680            0.068012             0.008356
81      4  AGGCAGCCAT  GGGGGGGGGG   2398519               0.011973        0.000637            0.063651             0.007820
82      4  CAACGTTGAC  GGGGGGGGGG   2344071               0.011701        0.000622            0.062206             0.007643
83      4  ATGATCAACG  GGGGGGGGGG   2256026               0.011262        0.000599            0.059869             0.007356
84      4  GCGACTCGTA  GGGGGGGGGG   2234566               0.011154        0.000593            0.059300             0.007286
85      4  CCTGGAATTA  TGTGTACCAG   2126765               0.010616        0.000564            0.056439             0.006934
86      4  CCTATGTGTA  GGGGGGGGGG   2114862               0.010557        0.000561            0.056123             0.006896
87      4  TTCAGATCCA  GGGGGGGGGG   2042821               0.010197        0.000542            0.054211             0.006661
88      4  GCGCGGTTAA  GGGGGGGGGG   1980505               0.009886        0.000526            0.052558             0.006457
89      4  CCTGGAATTA  GTACTCTCTA   1892375               0.009446        0.000502            0.050219             0.006170
90      5  CCAGTGCACT  GGGGGGGGGG   8539896               0.049701        0.002353            0.235339             0.027845
91      5  TTGGATTGTC  GGGGGGGGGG   4910547               0.028579        0.001353            0.135323             0.016011
92      5  CATCAGGCGT  GGGGGGGGGG   4439230               0.025836        0.001223            0.122335             0.014474
93      5  ACAATAGCAC  GGGGGGGGGG   3094884               0.018012        0.000853            0.085288             0.010091
94      5  AATTGGCACA  GGGGGGGGGG   2733163               0.015907        0.000753            0.075320             0.008912
95      5  GTACGCATCG  GGGGGGGGGG   2701236               0.015721        0.000744            0.074440             0.008807
96      5  TGCAACACTA  GGGGGGGGGG   2660121               0.015481        0.000733            0.073307             0.008673
97      5  GAAGCAAGCG  GGGGGGGGGG   1876059               0.010918        0.000517            0.051700             0.006117
98      5  CCACCTGCTT  GGGGGGGGGG   1827986               0.010639        0.000504            0.050375             0.005960
99      6  CCAGTGCACT  GGGGGGGGGG  11955380               0.064923        0.003086            0.308593             0.038981
100     6  TTGGATTGTC  GGGGGGGGGG   6822504               0.037049        0.001761            0.176103             0.022245
101     6  CATCAGGCGT  GGGGGGGGGG   6072888               0.032979        0.001568            0.156754             0.019801
102     6  ACAATAGCAC  GGGGGGGGGG   4081883               0.022167        0.001054            0.105362             0.013309
103     6  AATTGGCACA  GGGGGGGGGG   3718466               0.020193        0.000960            0.095981             0.012124
104     6  GTACGCATCG  GGGGGGGGGG   3573840               0.019408        0.000922            0.092248             0.011653
105     6  TGCAACACTA  GGGGGGGGGG   3383154               0.018372        0.000873            0.087326             0.011031
106     6  GAAGCAAGCG  GGGGGGGGGG   2685584               0.014584        0.000693            0.069320             0.008756
107     6  CCACCTGCTT  GGGGGGGGGG   2217437               0.012042        0.000572            0.057237             0.007230
108     6  TCGCATGACA  GGGGGGGGGG   2075737               0.011272        0.000536            0.053579             0.006768
109     7  CCAGTGCACT  GGGGGGGGGG  12520817               0.065846        0.003252            0.325160             0.040824
110     7  TTGGATTGTC  GGGGGGGGGG   7190619               0.037815        0.001867            0.186737             0.023445
111     7  CATCAGGCGT  GGGGGGGGGG   6398853               0.033651        0.001662            0.166175             0.020864
112     7  ACAATAGCAC  GGGGGGGGGG   4262477               0.022416        0.001107            0.110694             0.013898
113     7  AATTGGCACA  GGGGGGGGGG   3935036               0.020694        0.001022            0.102191             0.012830
114     7  GTACGCATCG  GGGGGGGGGG   3781413               0.019886        0.000982            0.098201             0.012329
115     7  TGCAACACTA  GGGGGGGGGG   3522018               0.018522        0.000915            0.091465             0.011484
116     7  GAAGCAAGCG  GGGGGGGGGG   2870324               0.015095        0.000745            0.074541             0.009359
117     7  CCACCTGCTT  GGGGGGGGGG   2310566               0.012151        0.000600            0.060004             0.007534
118     7  TCGCATGACA  GGGGGGGGGG   2172931               0.011427        0.000564            0.056430             0.007085
119     7  AGTACAACTG  GGGGGGGGGG   1973818               0.010380        0.000513            0.051259             0.006436
120     8  CCAGTGCACT  GGGGGGGGGG  13808902               0.068287        0.003474            0.347433             0.045024
121     8  TTGGATTGTC  GGGGGGGGGG   8055516               0.039836        0.002027            0.202677             0.026265
122     8  CATCAGGCGT  GGGGGGGGGG   7292412               0.036062        0.001835            0.183477             0.023777
123     8  ACAATAGCAC  GGGGGGGGGG   4755613               0.023517        0.001197            0.119651             0.015506
124     8  AATTGGCACA  GGGGGGGGGG   4462422               0.022067        0.001123            0.112275             0.014550
125     8  GTACGCATCG  GGGGGGGGGG   4370742               0.021614        0.001100            0.109968             0.014251
126     8  TGCAACACTA  GGGGGGGGGG   3938302               0.019476        0.000991            0.099088             0.012841
127     8  GAAGCAAGCG  GGGGGGGGGG   3465446               0.017137        0.000872            0.087191             0.011299
128     8  CCACCTGCTT  GGGGGGGGGG   2611613               0.012915        0.000657            0.065708             0.008515
129     8  TCGCATGACA  GGGGGGGGGG   2436248               0.012048        0.000613            0.061296             0.007943
130     8  AGTACAACTG  GGGGGGGGGG   2300837               0.011378        0.000579            0.057889             0.007502
131     8  GCCGTGAACA  GGGGGGGGGG   2057642               0.010175        0.000518            0.051770             0.006709

5. BARCODES WITH HIGH MISMATCH RATES
--------------------------------------------------------------------------------
No barcodes found with high mismatch rates

================================================================================
FINAL STATUS CHECK
================================================================================
Overall % Undetermined: 5.20%
Status: FAIL
Reason(s):
  Overall undetermined 5.20% >= 5.0%
  NTC sample NTC_0001_0001_23KMKGLT4_1 has 3,096,639 reads > 2,000,000