Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 100000000 total bases: 15100000000 Q20 bases: 14815838143(98.1181%) Q30 bases: 14322946307(94.8539%) Read2 before filtering: total reads: 100000000 total bases: 15100000000 Q20 bases: 14743765335(97.6408%) Q30 bases: 14095135972(93.3453%) Read1 after filtering: total reads: 97275510 total bases: 12109817027 Q20 bases: 12057288139(99.5662%) Q30 bases: 11859740105(97.9349%) Read2 after filtering: total reads: 97275510 total bases: 12126761053 Q20 bases: 12033899895(99.2342%) Q30 bases: 11763488246(97.0044%) Filtering result: reads passed filter: 194551020 reads failed due to low quality: 3211322 reads failed due to too many N: 451012 reads failed due to too short: 1786646 reads with adapter trimmed: 127096290 bases trimmed due to adapters: 5233570456 Duplication rate: 34.7775% Insert size peak (evaluated by paired-end reads): 109 JSON report: GM24385_0001_RNA_0001_23H5VFLT4_s48.fastp.json HTML report: GM24385_0001_RNA_0001_23H5VFLT4_s48.fastp.html fastp --in1 GM24385_0001_RNA_0001_23H5VFLT4_s48_1.fastq.gz --in2 GM24385_0001_RNA_0001_23H5VFLT4_s48_2.fastq.gz --out1 GM24385_0001_RNA_0001_23H5VFLT4_s48_1.fastp.fastq.gz --out2 GM24385_0001_RNA_0001_23H5VFLT4_s48_2.fastp.fastq.gz --json GM24385_0001_RNA_0001_23H5VFLT4_s48.fastp.json --html GM24385_0001_RNA_0001_23H5VFLT4_s48.fastp.html --thread 12 --detect_adapter_for_pe fastp v0.23.4, time used: 202 seconds