FastQCFastQC Report
Wed 4 Mar 2026
GM24385_0001_RNA_0001_23H5VFLT4_s48_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGM24385_0001_RNA_0001_23H5VFLT4_s48_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100000000
Total Bases15.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGCGCATCTAGTAT3672770.367277TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGCGCATCTGGTAT2302900.23029TruSeq Adapter, Index 15 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)