Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 206994 total bases: 31256094 Q20 bases: 26891816(86.037%) Q30 bases: 20602925(65.9165%) Read2 before filtering: total reads: 206994 total bases: 31256094 Q20 bases: 29573386(94.6164%) Q30 bases: 23955746(76.6434%) Read1 after filtering: total reads: 36441 total bases: 2341075 Q20 bases: 2229678(95.2416%) Q30 bases: 2045946(87.3934%) Read2 after filtering: total reads: 36441 total bases: 2412120 Q20 bases: 2353604(97.5741%) Q30 bases: 2211383(91.678%) Filtering result: reads passed filter: 72882 reads failed due to low quality: 13762 reads failed due to too many N: 2 reads failed due to too short: 327342 reads with adapter trimmed: 231627 bases trimmed due to adapters: 12444608 Duplication rate: 43.6114% Insert size peak (evaluated by paired-end reads): 35 JSON report: NTC_0001_0001_23H5VFLT4_s15.fastp.json HTML report: NTC_0001_0001_23H5VFLT4_s15.fastp.html fastp --in1 NTC_0001_0001_23H5VFLT4_s15_1.fastq.gz --in2 NTC_0001_0001_23H5VFLT4_s15_2.fastq.gz --out1 NTC_0001_0001_23H5VFLT4_s15_1.fastp.fastq.gz --out2 NTC_0001_0001_23H5VFLT4_s15_2.fastp.fastq.gz --json NTC_0001_0001_23H5VFLT4_s15.fastp.json --html NTC_0001_0001_23H5VFLT4_s15.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 4 seconds