[W::hts_idx_load3] The index file is older than the data file: HD789_0002_RNA_0008_23H5VFLT4_s19.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (17860217) splicing reads. 290049 splicing junctions. 189208 known splicing junctions. 100841 novel splicing junctions.
sampling 10% (35720435) splicing reads. 360732 splicing junctions. 203074 known splicing junctions. 157658 novel splicing junctions.
sampling 15% (53580653) splicing reads. 410611 splicing junctions. 210355 known splicing junctions. 200256 novel splicing junctions.
sampling 20% (71440871) splicing reads. 449684 splicing junctions. 215187 known splicing junctions. 234497 novel splicing junctions.
sampling 25% (89301089) splicing reads. 481502 splicing junctions. 218653 known splicing junctions. 262849 novel splicing junctions.
sampling 30% (107161307) splicing reads. 508373 splicing junctions. 221378 known splicing junctions. 286995 novel splicing junctions.
sampling 35% (125021525) splicing reads. 531644 splicing junctions. 223485 known splicing junctions. 308159 novel splicing junctions.
sampling 40% (142881743) splicing reads. 551895 splicing junctions. 225303 known splicing junctions. 326592 novel splicing junctions.
sampling 45% (160741961) splicing reads. 569651 splicing junctions. 226788 known splicing junctions. 342863 novel splicing junctions.
sampling 50% (178602179) splicing reads. 585355 splicing junctions. 228085 known splicing junctions. 357270 novel splicing junctions.
sampling 55% (196462397) splicing reads. 599238 splicing junctions. 229115 known splicing junctions. 370123 novel splicing junctions.
sampling 60% (214322615) splicing reads. 611929 splicing junctions. 230054 known splicing junctions. 381875 novel splicing junctions.
sampling 65% (232182833) splicing reads. 623167 splicing junctions. 230808 known splicing junctions. 392359 novel splicing junctions.
sampling 70% (250043051) splicing reads. 633146 splicing junctions. 231458 known splicing junctions. 401688 novel splicing junctions.
sampling 75% (267903269) splicing reads. 641947 splicing junctions. 232081 known splicing junctions. 409866 novel splicing junctions.
sampling 80% (285763487) splicing reads. 649893 splicing junctions. 232609 known splicing junctions. 417284 novel splicing junctions.
sampling 85% (303623705) splicing reads. 656786 splicing junctions. 233100 known splicing junctions. 423686 novel splicing junctions.
sampling 90% (321483923) splicing reads. 663031 splicing junctions. 233492 known splicing junctions. 429539 novel splicing junctions.
sampling 95% (339344141) splicing reads. 668300 splicing junctions. 233828 known splicing junctions. 434472 novel splicing junctions.
sampling 100% (357204359) splicing reads. 672699 splicing junctions. 234119 known splicing junctions. 438580 novel splicing junctions.