[W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s32.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (9819693) splicing reads. 206460 splicing junctions. 152291 known splicing junctions. 54169 novel splicing junctions.
sampling 10% (19639386) splicing reads. 241386 splicing junctions. 161750 known splicing junctions. 79636 novel splicing junctions.
sampling 15% (29459079) splicing reads. 262431 splicing junctions. 166387 known splicing junctions. 96044 novel splicing junctions.
sampling 20% (39278772) splicing reads. 276942 splicing junctions. 169159 known splicing junctions. 107783 novel splicing junctions.
sampling 25% (49098465) splicing reads. 287744 splicing junctions. 171042 known splicing junctions. 116702 novel splicing junctions.
sampling 30% (58918158) splicing reads. 296185 splicing junctions. 172428 known splicing junctions. 123757 novel splicing junctions.
sampling 35% (68737851) splicing reads. 302986 splicing junctions. 173504 known splicing junctions. 129482 novel splicing junctions.
sampling 40% (78557544) splicing reads. 308562 splicing junctions. 174397 known splicing junctions. 134165 novel splicing junctions.
sampling 45% (88377237) splicing reads. 313192 splicing junctions. 175060 known splicing junctions. 138132 novel splicing junctions.
sampling 50% (98196930) splicing reads. 317054 splicing junctions. 175557 known splicing junctions. 141497 novel splicing junctions.
sampling 55% (108016623) splicing reads. 320387 splicing junctions. 176016 known splicing junctions. 144371 novel splicing junctions.
sampling 60% (117836316) splicing reads. 323237 splicing junctions. 176399 known splicing junctions. 146838 novel splicing junctions.
sampling 65% (127656009) splicing reads. 325620 splicing junctions. 176702 known splicing junctions. 148918 novel splicing junctions.
sampling 70% (137475702) splicing reads. 327724 splicing junctions. 176961 known splicing junctions. 150763 novel splicing junctions.
sampling 75% (147295395) splicing reads. 329495 splicing junctions. 177171 known splicing junctions. 152324 novel splicing junctions.
sampling 80% (157115088) splicing reads. 330965 splicing junctions. 177329 known splicing junctions. 153636 novel splicing junctions.
sampling 85% (166934781) splicing reads. 332161 splicing junctions. 177465 known splicing junctions. 154696 novel splicing junctions.
sampling 90% (176754474) splicing reads. 333094 splicing junctions. 177579 known splicing junctions. 155515 novel splicing junctions.
sampling 95% (186574167) splicing reads. 333825 splicing junctions. 177673 known splicing junctions. 156152 novel splicing junctions.
sampling 100% (196393860) splicing reads. 334338 splicing junctions. 177737 known splicing junctions. 156601 novel splicing junctions.