08:45:35 : INFO : -reading targets list: ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-mapping reads to genes
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[15740000], rate=16006779.66/min
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[15760000], rate=16027118.64/min
[15770000], rate=16037288.14/min
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[15830000], rate=16098305.08/min
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[15860000], rate=16128813.56/min
[15870000], rate=16138983.05/min
[15880000], rate=16149152.54/min
[15890000], rate=16159322.03/min
[15900000], rate=16169491.53/min
[15910000], rate=16179661.02/min
[15920000], rate=16189830.51/min
[15930000], rate=15930000.00/min
[15940000], rate=15940000.00/min
[15950000], rate=15950000.00/min
[15960000], rate=15960000.00/min
[15970000], rate=15970000.00/min
[15980000], rate=15980000.00/min
[15990000], rate=15990000.00/min
[16000000], rate=16000000.00/min
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[16020000], rate=16020000.00/min
[16030000], rate=16030000.00/min
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[16120000], rate=16120000.00/min
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[16150000], rate=16150000.00/min
[16160000], rate=16160000.00/min
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[16190000], rate=16190000.00/min
[16200000], rate=16200000.00/min
[16210000], rate=16210000.00/min
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[16240000], rate=16240000.00/min
[16250000], rate=16250000.00/min
[16260000], rate=15993442.62/min
[16270000], rate=16003278.69/min
[16280000], rate=16013114.75/min
[16290000], rate=16022950.82/min
[16300000], rate=16032786.89/min
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[16340000], rate=16072131.15/min
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[16390000], rate=16121311.48/min
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[16470000], rate=16200000.00/min
[16480000], rate=16209836.07/min
[16490000], rate=16219672.13/min
[16500000], rate=16229508.20/min
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[16540000], rate=16268852.46/min
[16550000], rate=16278688.52/min 08:46:45 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file tih_rna_sample_00377_23H5VFLT4_s23.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > tih_rna_sample_00377_23H5VFLT4_s23.cancer.introns.prelim
Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18.
-tih_rna_sample_00377_23H5VFLT4_s23.introns: identified 6991 cancer introns
08:46:46 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file tih_rna_sample_00377_23H5VFLT4_s23.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > tih_rna_sample_00377_23H5VFLT4_s23.cancer.introns.prelim
08:46:46 : INFO : Running: touch /tmp/nxf.K3Lswu3n1Z/tih_rna_sample_00377_23H5VFLT4_s23.chckpts/prelim_introns.ok
08:46:46 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates tih_rna_sample_00377_23H5VFLT4_s23.cancer.introns.prelim --min_total_reads 5 > tih_rna_sample_00377_23H5VFLT4_s23.cancer.introns
08:46:46 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates tih_rna_sample_00377_23H5VFLT4_s23.cancer.introns.prelim --min_total_reads 5 > tih_rna_sample_00377_23H5VFLT4_s23.cancer.introns
08:46:46 : INFO : Running: touch /tmp/nxf.K3Lswu3n1Z/tih_rna_sample_00377_23H5VFLT4_s23.chckpts/introns_filtered.ok
08:46:46 : INFO : -found 2872 cancer introns
08:46:46 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns tih_rna_sample_00377_23H5VFLT4_s23.introns --cancer_introns tih_rna_sample_00377_23H5VFLT4_s23.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed tih_rna_sample_00377_23H5VFLT4_s23.introns.for_IGV.bed
08:46:46 : INFO : Creating the BED File.
08:46:53 : INFO : Saving Bed File as tih_rna_sample_00377_23H5VFLT4_s23.introns.for_IGV.bed
08:46:53 : INFO : Execution Time = 0.11 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns tih_rna_sample_00377_23H5VFLT4_s23.introns --cancer_introns tih_rna_sample_00377_23H5VFLT4_s23.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed tih_rna_sample_00377_23H5VFLT4_s23.introns.for_IGV.bed
08:46:53 : INFO : Running: touch /tmp/nxf.K3Lswu3n1Z/tih_rna_sample_00377_23H5VFLT4_s23.chckpts/intron_igv_bed.ok
08:46:53 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed tih_rna_sample_00377_23H5VFLT4_s23.introns.for_IGV.bed --bam tih_rna_sample_00377_23H5VFLT4_s23.Aligned.sortedByCoord.out.bam --output_prefix tih_rna_sample_00377_23H5VFLT4_s23
[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00377_23H5VFLT4_s23.Aligned.sortedByCoord.out.bam.bai
09:13:56 : INFO : Execution Time = 27.05 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed tih_rna_sample_00377_23H5VFLT4_s23.introns.for_IGV.bed --bam tih_rna_sample_00377_23H5VFLT4_s23.Aligned.sortedByCoord.out.bam --output_prefix tih_rna_sample_00377_23H5VFLT4_s23
09:13:56 : INFO : Running: touch /tmp/nxf.K3Lswu3n1Z/tih_rna_sample_00377_23H5VFLT4_s23.chckpts/reads_alignments_extracted.ok
09:13:56 : INFO : Running: samtools sort -o tih_rna_sample_00377_23H5VFLT4_s23.cancer_intron_reads.sorted.bam tih_rna_sample_00377_23H5VFLT4_s23.cancer_intron_reads.bam
09:13:57 : INFO : Execution Time = 0.01 minutes. CMD: samtools sort -o tih_rna_sample_00377_23H5VFLT4_s23.cancer_intron_reads.sorted.bam tih_rna_sample_00377_23H5VFLT4_s23.cancer_intron_reads.bam
09:13:57 : INFO : Running: touch /tmp/nxf.K3Lswu3n1Z/tih_rna_sample_00377_23H5VFLT4_s23.chckpts/sort_cancer_intron_reads.ok
09:13:57 : INFO : Running: samtools sort -o tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.sorted.bam tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.bam
[bam_sort_core] merging from 128 files and 1 in-memory blocks...
09:44:26 : INFO : Execution Time = 30.49 minutes. CMD: samtools sort -o tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.sorted.bam tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.bam
09:44:26 : INFO : Running: touch /tmp/nxf.K3Lswu3n1Z/tih_rna_sample_00377_23H5VFLT4_s23.chckpts/sort_gene_reads.ok
09:44:26 : INFO : Running: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.sorted.sifted.bam.tmp --keep_secondary tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.sorted.bam
09:54:05 : INFO : Execution Time = 9.64 minutes. CMD: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.sorted.sifted.bam.tmp --keep_secondary tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.sorted.bam
09:54:05 : INFO : Running: touch /tmp/nxf.K3Lswu3n1Z/tih_rna_sample_00377_23H5VFLT4_s23.chckpts/tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.sorted.sifted.bam.tmp.ok
09:54:05 : INFO : Running: samtools sort -o tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.sorted.sifted.bam tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.sorted.sifted.bam.tmp
[bam_sort_core] merging from 7 files and 1 in-memory blocks...
09:55:59 : INFO : Execution Time = 1.90 minutes. CMD: samtools sort -o tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.sorted.sifted.bam tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.sorted.sifted.bam.tmp
09:55:59 : INFO : Running: touch /tmp/nxf.K3Lswu3n1Z/tih_rna_sample_00377_23H5VFLT4_s23.chckpts/tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.sorted.sifted.bam.tmpsorted.ok
09:55:59 : INFO : Running: samtools index tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.sorted.sifted.bam
09:56:11 : INFO : Execution Time = 0.20 minutes. CMD: samtools index tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.sorted.sifted.bam
09:56:11 : INFO : Running: touch /tmp/nxf.K3Lswu3n1Z/tih_rna_sample_00377_23H5VFLT4_s23.chckpts/tih_rna_sample_00377_23H5VFLT4_s23.gene_reads.sorted.sifted.bam.indexed.ok
09:56:11 : INFO : Running: samtools index tih_rna_sample_00377_23H5VFLT4_s23.cancer_intron_reads.sorted.bam
09:56:11 : INFO : Execution Time = 0.00 minutes. CMD: samtools index tih_rna_sample_00377_23H5VFLT4_s23.cancer_intron_reads.sorted.bam
09:56:11 : INFO : Running: touch /tmp/nxf.K3Lswu3n1Z/tih_rna_sample_00377_23H5VFLT4_s23.chckpts/tih_rna_sample_00377_23H5VFLT4_s23.cancer_intron_reads.sorted.bam.indexed.ok
09:56:11 : INFO : Running: create_report tih_rna_sample_00377_23H5VFLT4_s23.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output tih_rna_sample_00377_23H5VFLT4_s23.ctat-splicing.igv.html --track-config tih_rna_sample_00377_23H5VFLT4_s23.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: tih_rna_sample_00377_23H5VFLT4_s23'
10:04:11 : INFO : Execution Time = 7.99 minutes. CMD: create_report tih_rna_sample_00377_23H5VFLT4_s23.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output tih_rna_sample_00377_23H5VFLT4_s23.ctat-splicing.igv.html --track-config tih_rna_sample_00377_23H5VFLT4_s23.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: tih_rna_sample_00377_23H5VFLT4_s23'
10:04:11 : INFO : Running: touch /tmp/nxf.K3Lswu3n1Z/tih_rna_sample_00377_23H5VFLT4_s23.chckpts/igv_create_html.ok
10:04:11 : INFO : done.