[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00116_23H5VFLT4_s36.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14141679) splicing reads. 268959 splicing junctions. 187193 known splicing junctions. 81766 novel splicing junctions.
sampling 10% (28283358) splicing reads. 333271 splicing junctions. 200203 known splicing junctions. 133068 novel splicing junctions.
sampling 15% (42425037) splicing reads. 378820 splicing junctions. 207100 known splicing junctions. 171720 novel splicing junctions.
sampling 20% (56566716) splicing reads. 414345 splicing junctions. 211711 known splicing junctions. 202634 novel splicing junctions.
sampling 25% (70708395) splicing reads. 443105 splicing junctions. 214997 known splicing junctions. 228108 novel splicing junctions.
sampling 30% (84850074) splicing reads. 467068 splicing junctions. 217502 known splicing junctions. 249566 novel splicing junctions.
sampling 35% (98991753) splicing reads. 487088 splicing junctions. 219382 known splicing junctions. 267706 novel splicing junctions.
sampling 40% (113133432) splicing reads. 504478 splicing junctions. 220960 known splicing junctions. 283518 novel splicing junctions.
sampling 45% (127275111) splicing reads. 519365 splicing junctions. 222277 known splicing junctions. 297088 novel splicing junctions.
sampling 50% (141416791) splicing reads. 531950 splicing junctions. 223300 known splicing junctions. 308650 novel splicing junctions.
sampling 55% (155558470) splicing reads. 542904 splicing junctions. 224114 known splicing junctions. 318790 novel splicing junctions.
sampling 60% (169700149) splicing reads. 552486 splicing junctions. 224889 known splicing junctions. 327597 novel splicing junctions.
sampling 65% (183841828) splicing reads. 560695 splicing junctions. 225520 known splicing junctions. 335175 novel splicing junctions.
sampling 70% (197983507) splicing reads. 567697 splicing junctions. 226107 known splicing junctions. 341590 novel splicing junctions.
sampling 75% (212125186) splicing reads. 573544 splicing junctions. 226541 known splicing junctions. 347003 novel splicing junctions.
sampling 80% (226266865) splicing reads. 578437 splicing junctions. 226911 known splicing junctions. 351526 novel splicing junctions.
sampling 85% (240408544) splicing reads. 582489 splicing junctions. 227188 known splicing junctions. 355301 novel splicing junctions.
sampling 90% (254550223) splicing reads. 585686 splicing junctions. 227418 known splicing junctions. 358268 novel splicing junctions.
sampling 95% (268691902) splicing reads. 588046 splicing junctions. 227606 known splicing junctions. 360440 novel splicing junctions.
sampling 100% (282833582) splicing reads. 589492 splicing junctions. 227716 known splicing junctions. 361776 novel splicing junctions.