[W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s08.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (12609051) splicing reads. 230239 splicing junctions. 159723 known splicing junctions. 70516 novel splicing junctions.
sampling 10% (25218102) splicing reads. 278026 splicing junctions. 170963 known splicing junctions. 107063 novel splicing junctions.
sampling 15% (37827154) splicing reads. 309396 splicing junctions. 176738 known splicing junctions. 132658 novel splicing junctions.
sampling 20% (50436205) splicing reads. 332255 splicing junctions. 180416 known splicing junctions. 151839 novel splicing junctions.
sampling 25% (63045256) splicing reads. 349986 splicing junctions. 183065 known splicing junctions. 166921 novel splicing junctions.
sampling 30% (75654308) splicing reads. 364268 splicing junctions. 184978 known splicing junctions. 179290 novel splicing junctions.
sampling 35% (88263359) splicing reads. 376057 splicing junctions. 186502 known splicing junctions. 189555 novel splicing junctions.
sampling 40% (100872410) splicing reads. 385728 splicing junctions. 187678 known splicing junctions. 198050 novel splicing junctions.
sampling 45% (113481462) splicing reads. 393839 splicing junctions. 188635 known splicing junctions. 205204 novel splicing junctions.
sampling 50% (126090513) splicing reads. 400830 splicing junctions. 189368 known splicing junctions. 211462 novel splicing junctions.
sampling 55% (138699564) splicing reads. 406799 splicing junctions. 189995 known splicing junctions. 216804 novel splicing junctions.
sampling 60% (151308616) splicing reads. 412035 splicing junctions. 190527 known splicing junctions. 221508 novel splicing junctions.
sampling 65% (163917667) splicing reads. 416568 splicing junctions. 190997 known splicing junctions. 225571 novel splicing junctions.
sampling 70% (176526718) splicing reads. 420371 splicing junctions. 191397 known splicing junctions. 228974 novel splicing junctions.
sampling 75% (189135770) splicing reads. 423624 splicing junctions. 191717 known splicing junctions. 231907 novel splicing junctions.
sampling 80% (201744821) splicing reads. 426405 splicing junctions. 192000 known splicing junctions. 234405 novel splicing junctions.
sampling 85% (214353872) splicing reads. 428759 splicing junctions. 192251 known splicing junctions. 236508 novel splicing junctions.
sampling 90% (226962924) splicing reads. 430637 splicing junctions. 192419 known splicing junctions. 238218 novel splicing junctions.
sampling 95% (239571975) splicing reads. 432057 splicing junctions. 192572 known splicing junctions. 239485 novel splicing junctions.
sampling 100% (252181027) splicing reads. 433107 splicing junctions. 192667 known splicing junctions. 240440 novel splicing junctions.