+ mkdir GM24385_0001_RNA_0001_23H5VFLT4_s40 + all_qc -bam GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder GM24385_0001_RNA_0001_23H5VFLT4_s40 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai 0it [00:00, ?it/s] 71914it [00:00, 719022.40it/s] 161648it [00:00, 823864.60it/s] 248097it [00:00, 842379.32it/s] 339729it [00:00, 871533.45it/s] 426883it [00:00, 858776.28it/s] 512793it [00:00, 817707.05it/s] 613716it [00:00, 877837.80it/s] 701974it [00:00, 829243.25it/s] 785648it [00:00, 770335.05it/s] 877268it [00:01, 811214.91it/s] 971435it [00:01, 848503.06it/s] 1058380it [00:01, 854547.26it/s] 1144609it [00:01, 790593.59it/s] 1225030it [00:01, 744356.91it/s] 1300730it [00:01, 712803.56it/s] 1372938it [00:01, 686954.06it/s] 1442264it [00:01, 666329.20it/s] 1509291it [00:01, 647497.76it/s] 1574273it [00:02, 645684.60it/s] 1639074it [00:02, 646319.06it/s] 1705055it [00:02, 650157.55it/s] 1771672it 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749465.17it/s] 6259899it [00:08, 718650.87it/s] 6332114it [00:08, 713641.20it/s] 6414433it [00:08, 745451.35it/s] 6498062it [00:09, 772081.06it/s] 6581292it [00:09, 789841.72it/s] 6660479it [00:09, 772741.64it/s] 6737963it [00:09, 724602.64it/s] 6811106it [00:09, 698748.42it/s] 6881535it [00:09, 681248.38it/s] 6950040it [00:09, 662426.04it/s] 7026451it [00:09, 690806.41it/s] 7101939it [00:09, 709058.87it/s] 7174811it [00:10, 714636.54it/s] 7259343it [00:10, 752844.47it/s] 7336397it [00:10, 758038.07it/s] 7412417it [00:10, 723490.71it/s] 7485221it [00:10, 699930.44it/s] 7555623it [00:10, 685986.93it/s] 7624506it [00:10, 683313.91it/s] 7693025it [00:10, 668767.14it/s] 7760050it [00:10, 658421.80it/s] 7825990it [00:10, 653681.59it/s] 7891415it [00:11, 595248.03it/s] 7960146it [00:11, 620405.13it/s] 8038939it [00:11, 667401.54it/s] 8120442it [00:11, 709608.20it/s] 8198303it [00:11, 729567.80it/s] 8279633it [00:11, 754095.51it/s] 8362362it [00:11, 775649.52it/s] 8443430it [00:11, 786019.87it/s] 8522326it [00:11, 764730.74it/s] 8601879it [00:12, 773718.73it/s] 8684816it [00:12, 790097.00it/s] 8764030it [00:12, 740228.10it/s] 8842723it [00:12, 753392.12it/s] 8918659it [00:12, 748768.89it/s] 8993945it [00:12, 731585.15it/s] 9070578it [00:12, 741573.49it/s] 9148966it [00:12, 753882.98it/s] 9231121it [00:12, 773752.52it/s] 9312517it [00:12, 785622.51it/s] 9391236it [00:13, 746109.34it/s] 9474807it [00:13, 771810.25it/s] 9558845it [00:13, 791730.68it/s][W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai 9639497it [00:13, 796048.41it/s] 9719383it [00:13, 764739.95it/s] 9796285it [00:13, 733626.29it/s] 9877618it [00:13, 756154.64it/s] 9959847it [00:13, 775161.46it/s] 10045635it [00:13, 799184.51it/s] 10129840it [00:13, 811759.68it/s] 10216604it [00:14, 828237.19it/s] 10308713it [00:14, 855792.10it/s] 10407601it [00:14, 895333.57it/s] 10497289it [00:14, 808131.94it/s] 10579805it [00:14, 750769.75it/s] 10656642it [00:14, 734711.71it/s] 10738988it [00:14, 758782.49it/s] 10824851it [00:14, 786663.87it/s] 10907068it [00:14, 796645.59it/s] 10988992it [00:15, 803158.51it/s] 11069835it [00:15, 800858.67it/s] 11150287it [00:15, 800883.28it/s] 11230632it [00:15, 792743.73it/s] 11310094it [00:15, 755230.51it/s] 11387804it [00:15, 761461.69it/s] 11464299it [00:15, 757239.01it/s] 11540260it [00:15, 751731.04it/s] 11618859it [00:15, 761725.39it/s] 11706176it [00:15, 794534.93it/s] 11788983it [00:16, 804441.57it/s] 11873001it [00:16, 815052.39it/s] 11957392it [00:16, 823634.04it/s] 12039828it [00:16, 788745.73it/s][W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai 12120958it [00:16, 795266.14it/s] 12201877it [00:16, 799323.37it/s] 12282009it [00:16, 787677.51it/s] 12360938it [00:16, 787456.86it/s] 12443665it [00:16, 799189.64it/s] 12486999it [00:16, 735818.30it/s] [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai [W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.md.bai + merge_qc -folders GM24385_0001_RNA_0001_23H5VFLT4_s40/GM24385_0001_RNA_0001_23H5VFLT4_s40.md -output GM24385_0001_RNA_0001_23H5VFLT4_s40/GM24385_0001_RNA_0001_23H5VFLT4_s40_full_basic_stats.tsv + head -n1 GM24385_0001_RNA_0001_23H5VFLT4_s40/GM24385_0001_RNA_0001_23H5VFLT4_s40_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed GM24385_0001_RNA_0001_23H5VFLT4_s40/GM24385_0001_RNA_0001_23H5VFLT4_s40_full_basic_stats.tsv