Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/af/34079b33231d7fd5604b2bacd85b73/NTC_0001_0001_23H5VFLT4_s15_2.fastp.fastq.gz Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.gtf Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ea/7d4cf9a69da2d00bc38b19be96dda2/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2e/b98e3c81f942c27eced3763638b9e2/kallisto Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ea/7d4cf9a69da2d00bc38b19be96dda2/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/af/34079b33231d7fd5604b2bacd85b73/NTC_0001_0001_23H5VFLT4_s15_1.fastp.fastq.gz ==> STAGING COMPLETE (6 inputs) [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 194,360 [index] number of k-mers: 112,035,259 [quant] running in paired-end mode [quant] will process pair 1: NTC_0001_0001_23H5VFLT4_s15_1.fastp.fastq.gz NTC_0001_0001_23H5VFLT4_s15_2.fastp.fastq.gz [quant] finding pseudoalignments for the reads ... done [quant] processed 36,441 reads, 1,198 reads pseudoaligned [quant] estimated average fragment length: 32.9026 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 542 rounds