[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00117_23H5VFLT4_s37.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (9729066) splicing reads. 218348 splicing junctions. 175093 known splicing junctions. 43255 novel splicing junctions.
sampling 10% (19458133) splicing reads. 246245 splicing junctions. 184323 known splicing junctions. 61922 novel splicing junctions.
sampling 15% (29187199) splicing reads. 262187 splicing junctions. 188717 known splicing junctions. 73470 novel splicing junctions.
sampling 20% (38916266) splicing reads. 272597 splicing junctions. 191266 known splicing junctions. 81331 novel splicing junctions.
sampling 25% (48645332) splicing reads. 279852 splicing junctions. 193002 known splicing junctions. 86850 novel splicing junctions.
sampling 30% (58374399) splicing reads. 285550 splicing junctions. 194212 known splicing junctions. 91338 novel splicing junctions.
sampling 35% (68103465) splicing reads. 290134 splicing junctions. 195150 known splicing junctions. 94984 novel splicing junctions.
sampling 40% (77832532) splicing reads. 293895 splicing junctions. 195903 known splicing junctions. 97992 novel splicing junctions.
sampling 45% (87561598) splicing reads. 296957 splicing junctions. 196445 known splicing junctions. 100512 novel splicing junctions.
sampling 50% (97290665) splicing reads. 299496 splicing junctions. 196914 known splicing junctions. 102582 novel splicing junctions.
sampling 55% (107019731) splicing reads. 301639 splicing junctions. 197264 known splicing junctions. 104375 novel splicing junctions.
sampling 60% (116748798) splicing reads. 303513 splicing junctions. 197601 known splicing junctions. 105912 novel splicing junctions.
sampling 65% (126477864) splicing reads. 305140 splicing junctions. 197899 known splicing junctions. 107241 novel splicing junctions.
sampling 70% (136206931) splicing reads. 306541 splicing junctions. 198162 known splicing junctions. 108379 novel splicing junctions.
sampling 75% (145935997) splicing reads. 307737 splicing junctions. 198372 known splicing junctions. 109365 novel splicing junctions.
sampling 80% (155665064) splicing reads. 308721 splicing junctions. 198532 known splicing junctions. 110189 novel splicing junctions.
sampling 85% (165394130) splicing reads. 309524 splicing junctions. 198650 known splicing junctions. 110874 novel splicing junctions.
sampling 90% (175123197) splicing reads. 310152 splicing junctions. 198746 known splicing junctions. 111406 novel splicing junctions.
sampling 95% (184852263) splicing reads. 310601 splicing junctions. 198837 known splicing junctions. 111764 novel splicing junctions.
sampling 100% (194581330) splicing reads. 310887 splicing junctions. 198883 known splicing junctions. 112004 novel splicing junctions.