[W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s24.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (10171269) splicing reads. 214649 splicing junctions. 155300 known splicing junctions. 59349 novel splicing junctions.
sampling 10% (20342539) splicing reads. 258017 splicing junctions. 166795 known splicing junctions. 91222 novel splicing junctions.
sampling 15% (30513809) splicing reads. 286452 splicing junctions. 172706 known splicing junctions. 113746 novel splicing junctions.
sampling 20% (40685079) splicing reads. 307430 splicing junctions. 176522 known splicing junctions. 130908 novel splicing junctions.
sampling 25% (50856349) splicing reads. 323673 splicing junctions. 179175 known splicing junctions. 144498 novel splicing junctions.
sampling 30% (61027619) splicing reads. 336582 splicing junctions. 181092 known splicing junctions. 155490 novel splicing junctions.
sampling 35% (71198889) splicing reads. 347509 splicing junctions. 182691 known splicing junctions. 164818 novel splicing junctions.
sampling 40% (81370159) splicing reads. 356572 splicing junctions. 183939 known splicing junctions. 172633 novel splicing junctions.
sampling 45% (91541429) splicing reads. 364192 splicing junctions. 184948 known splicing junctions. 179244 novel splicing junctions.
sampling 50% (101712699) splicing reads. 370687 splicing junctions. 185778 known splicing junctions. 184909 novel splicing junctions.
sampling 55% (111883969) splicing reads. 376279 splicing junctions. 186416 known splicing junctions. 189863 novel splicing junctions.
sampling 60% (122055239) splicing reads. 381128 splicing junctions. 186970 known splicing junctions. 194158 novel splicing junctions.
sampling 65% (132226509) splicing reads. 385235 splicing junctions. 187447 known splicing junctions. 197788 novel splicing junctions.
sampling 70% (142397779) splicing reads. 388726 splicing junctions. 187876 known splicing junctions. 200850 novel splicing junctions.
sampling 75% (152569049) splicing reads. 391754 splicing junctions. 188213 known splicing junctions. 203541 novel splicing junctions.
sampling 80% (162740319) splicing reads. 394251 splicing junctions. 188493 known splicing junctions. 205758 novel splicing junctions.
sampling 85% (172911589) splicing reads. 396337 splicing junctions. 188721 known splicing junctions. 207616 novel splicing junctions.
sampling 90% (183082859) splicing reads. 398003 splicing junctions. 188886 known splicing junctions. 209117 novel splicing junctions.
sampling 95% (193254129) splicing reads. 399286 splicing junctions. 189030 known splicing junctions. 210256 novel splicing junctions.
sampling 100% (203425399) splicing reads. 400154 splicing junctions. 189129 known splicing junctions. 211025 novel splicing junctions.