[W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s16.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (13013511) splicing reads. 232677 splicing junctions. 160540 known splicing junctions. 72137 novel splicing junctions.
sampling 10% (26027022) splicing reads. 281132 splicing junctions. 171783 known splicing junctions. 109349 novel splicing junctions.
sampling 15% (39040534) splicing reads. 312670 splicing junctions. 177558 known splicing junctions. 135112 novel splicing junctions.
sampling 20% (52054045) splicing reads. 335869 splicing junctions. 181313 known splicing junctions. 154556 novel splicing junctions.
sampling 25% (65067557) splicing reads. 353657 splicing junctions. 183757 known splicing junctions. 169900 novel splicing junctions.
sampling 30% (78081068) splicing reads. 367827 splicing junctions. 185595 known splicing junctions. 182232 novel splicing junctions.
sampling 35% (91094579) splicing reads. 379480 splicing junctions. 187079 known splicing junctions. 192401 novel splicing junctions.
sampling 40% (104108091) splicing reads. 389207 splicing junctions. 188214 known splicing junctions. 200993 novel splicing junctions.
sampling 45% (117121602) splicing reads. 397264 splicing junctions. 189084 known splicing junctions. 208180 novel splicing junctions.
sampling 50% (130135114) splicing reads. 404390 splicing junctions. 189865 known splicing junctions. 214525 novel splicing junctions.
sampling 55% (143148625) splicing reads. 410476 splicing junctions. 190461 known splicing junctions. 220015 novel splicing junctions.
sampling 60% (156162136) splicing reads. 415805 splicing junctions. 191020 known splicing junctions. 224785 novel splicing junctions.
sampling 65% (169175648) splicing reads. 420244 splicing junctions. 191455 known splicing junctions. 228789 novel splicing junctions.
sampling 70% (182189159) splicing reads. 423956 splicing junctions. 191832 known splicing junctions. 232124 novel splicing junctions.
sampling 75% (195202671) splicing reads. 427183 splicing junctions. 192133 known splicing junctions. 235050 novel splicing junctions.
sampling 80% (208216182) splicing reads. 429894 splicing junctions. 192374 known splicing junctions. 237520 novel splicing junctions.
sampling 85% (221229693) splicing reads. 432141 splicing junctions. 192596 known splicing junctions. 239545 novel splicing junctions.
sampling 90% (234243205) splicing reads. 433995 splicing junctions. 192739 known splicing junctions. 241256 novel splicing junctions.
sampling 95% (247256716) splicing reads. 435462 splicing junctions. 192879 known splicing junctions. 242583 novel splicing junctions.
sampling 100% (260270228) splicing reads. 436510 splicing junctions. 192977 known splicing junctions. 243533 novel splicing junctions.