[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15009965) splicing reads. 277467 splicing junctions. 191252 known splicing junctions. 86215 novel splicing junctions.
sampling 10% (30019931) splicing reads. 342437 splicing junctions. 204567 known splicing junctions. 137870 novel splicing junctions.
sampling 15% (45029897) splicing reads. 388867 splicing junctions. 211832 known splicing junctions. 177035 novel splicing junctions.
sampling 20% (60039863) splicing reads. 425464 splicing junctions. 216611 known splicing junctions. 208853 novel splicing junctions.
sampling 25% (75049829) splicing reads. 455618 splicing junctions. 220110 known splicing junctions. 235508 novel splicing junctions.
sampling 30% (90059795) splicing reads. 481489 splicing junctions. 222822 known splicing junctions. 258667 novel splicing junctions.
sampling 35% (105069760) splicing reads. 503220 splicing junctions. 224894 known splicing junctions. 278326 novel splicing junctions.
sampling 40% (120079726) splicing reads. 522037 splicing junctions. 226647 known splicing junctions. 295390 novel splicing junctions.
sampling 45% (135089692) splicing reads. 538586 splicing junctions. 228082 known splicing junctions. 310504 novel splicing junctions.
sampling 50% (150099658) splicing reads. 553098 splicing junctions. 229273 known splicing junctions. 323825 novel splicing junctions.
sampling 55% (165109624) splicing reads. 565979 splicing junctions. 230295 known splicing junctions. 335684 novel splicing junctions.
sampling 60% (180119590) splicing reads. 577454 splicing junctions. 231147 known splicing junctions. 346307 novel splicing junctions.
sampling 65% (195129556) splicing reads. 587366 splicing junctions. 231890 known splicing junctions. 355476 novel splicing junctions.
sampling 70% (210139521) splicing reads. 596030 splicing junctions. 232518 known splicing junctions. 363512 novel splicing junctions.
sampling 75% (225149487) splicing reads. 603705 splicing junctions. 233086 known splicing junctions. 370619 novel splicing junctions.
sampling 80% (240159453) splicing reads. 610246 splicing junctions. 233575 known splicing junctions. 376671 novel splicing junctions.
sampling 85% (255169419) splicing reads. 615878 splicing junctions. 233974 known splicing junctions. 381904 novel splicing junctions.
sampling 90% (270179385) splicing reads. 620519 splicing junctions. 234310 known splicing junctions. 386209 novel splicing junctions.
sampling 95% (285189351) splicing reads. 624377 splicing junctions. 234597 known splicing junctions. 389780 novel splicing junctions.
sampling 100% (300199317) splicing reads. 627455 splicing junctions. 234843 known splicing junctions. 392612 novel splicing junctions.