[W::hts_idx_load3] The index file is older than the data file: GM24385_0001_RNA_0001_23H5VFLT4_s40.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (10838420) splicing reads. 206523 splicing junctions. 152539 known splicing junctions. 53984 novel splicing junctions.
sampling 10% (21676840) splicing reads. 238467 splicing junctions. 161216 known splicing junctions. 77251 novel splicing junctions.
sampling 15% (32515261) splicing reads. 256701 splicing junctions. 165271 known splicing junctions. 91430 novel splicing junctions.
sampling 20% (43353681) splicing reads. 268834 splicing junctions. 167718 known splicing junctions. 101116 novel splicing junctions.
sampling 25% (54192102) splicing reads. 278061 splicing junctions. 169287 known splicing junctions. 108774 novel splicing junctions.
sampling 30% (65030522) splicing reads. 285069 splicing junctions. 170473 known splicing junctions. 114596 novel splicing junctions.
sampling 35% (75868943) splicing reads. 290628 splicing junctions. 171325 known splicing junctions. 119303 novel splicing junctions.
sampling 40% (86707363) splicing reads. 295198 splicing junctions. 172005 known splicing junctions. 123193 novel splicing junctions.
sampling 45% (97545784) splicing reads. 299103 splicing junctions. 172565 known splicing junctions. 126538 novel splicing junctions.
sampling 50% (108384204) splicing reads. 302294 splicing junctions. 173001 known splicing junctions. 129293 novel splicing junctions.
sampling 55% (119222624) splicing reads. 305003 splicing junctions. 173368 known splicing junctions. 131635 novel splicing junctions.
sampling 60% (130061045) splicing reads. 307312 splicing junctions. 173657 known splicing junctions. 133655 novel splicing junctions.
sampling 65% (140899465) splicing reads. 309303 splicing junctions. 173917 known splicing junctions. 135386 novel splicing junctions.
sampling 70% (151737886) splicing reads. 311031 splicing junctions. 174125 known splicing junctions. 136906 novel splicing junctions.
sampling 75% (162576306) splicing reads. 312410 splicing junctions. 174282 known splicing junctions. 138128 novel splicing junctions.
sampling 80% (173414727) splicing reads. 313611 splicing junctions. 174416 known splicing junctions. 139195 novel splicing junctions.
sampling 85% (184253147) splicing reads. 314584 splicing junctions. 174524 known splicing junctions. 140060 novel splicing junctions.
sampling 90% (195091568) splicing reads. 315324 splicing junctions. 174619 known splicing junctions. 140705 novel splicing junctions.
sampling 95% (205929988) splicing reads. 315914 splicing junctions. 174693 known splicing junctions. 141221 novel splicing junctions.
sampling 100% (216768409) splicing reads. 316284 splicing junctions. 174745 known splicing junctions. 141539 novel splicing junctions.