[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00090_23H5VFLT4_s32.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (13426258) splicing reads. 252031 splicing junctions. 175005 known splicing junctions. 77026 novel splicing junctions.
sampling 10% (26852516) splicing reads. 312152 splicing junctions. 187959 known splicing junctions. 124193 novel splicing junctions.
sampling 15% (40278774) splicing reads. 354897 splicing junctions. 194978 known splicing junctions. 159919 novel splicing junctions.
sampling 20% (53705033) splicing reads. 388331 splicing junctions. 199670 known splicing junctions. 188661 novel splicing junctions.
sampling 25% (67131291) splicing reads. 415237 splicing junctions. 202958 known splicing junctions. 212279 novel splicing junctions.
sampling 30% (80557549) splicing reads. 437979 splicing junctions. 205530 known splicing junctions. 232449 novel splicing junctions.
sampling 35% (93983807) splicing reads. 456981 splicing junctions. 207527 known splicing junctions. 249454 novel splicing junctions.
sampling 40% (107410066) splicing reads. 473475 splicing junctions. 209132 known splicing junctions. 264343 novel splicing junctions.
sampling 45% (120836324) splicing reads. 487808 splicing junctions. 210498 known splicing junctions. 277310 novel splicing junctions.
sampling 50% (134262582) splicing reads. 500070 splicing junctions. 211579 known splicing junctions. 288491 novel splicing junctions.
sampling 55% (147688840) splicing reads. 510498 splicing junctions. 212526 known splicing junctions. 297972 novel splicing junctions.
sampling 60% (161115099) splicing reads. 519769 splicing junctions. 213284 known splicing junctions. 306485 novel splicing junctions.
sampling 65% (174541357) splicing reads. 527569 splicing junctions. 213948 known splicing junctions. 313621 novel splicing junctions.
sampling 70% (187967615) splicing reads. 534176 splicing junctions. 214545 known splicing junctions. 319631 novel splicing junctions.
sampling 75% (201393873) splicing reads. 539933 splicing junctions. 215005 known splicing junctions. 324928 novel splicing junctions.
sampling 80% (214820132) splicing reads. 544841 splicing junctions. 215373 known splicing junctions. 329468 novel splicing junctions.
sampling 85% (228246390) splicing reads. 548792 splicing junctions. 215677 known splicing junctions. 333115 novel splicing junctions.
sampling 90% (241672648) splicing reads. 551863 splicing junctions. 215922 known splicing junctions. 335941 novel splicing junctions.
sampling 95% (255098906) splicing reads. 554206 splicing junctions. 216093 known splicing junctions. 338113 novel splicing junctions.
sampling 100% (268525165) splicing reads. 555769 splicing junctions. 216229 known splicing junctions. 339540 novel splicing junctions.