Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2f/9055d4b0da3d2fb2a1c6cf662c7616/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f6/8de8aa4f3506756f32b934326968f7/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f6/8de8aa4f3506756f32b934326968f7/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4a/708828745aa92ea96e9a7231bb51c7/tih_rna_sample_00105_23H5VFLT4_s21.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/97/f63ee4c7bdb5db7845b878969ce73b/tih_rna_sample_00105_23H5VFLT4_s21.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00105_23H5VFLT4_s21.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (1349516) splicing reads. 138635 splicing junctions. 126796 known splicing junctions. 11839 novel splicing junctions.
sampling 10% (2699033) splicing reads. 156772 splicing junctions. 138679 known splicing junctions. 18093 novel splicing junctions.
sampling 15% (4048550) splicing reads. 166317 splicing junctions. 143908 known splicing junctions. 22409 novel splicing junctions.
sampling 20% (5398066) splicing reads. 172509 splicing junctions. 146910 known splicing junctions. 25599 novel splicing junctions.
sampling 25% (6747583) splicing reads. 176942 splicing junctions. 148810 known splicing junctions. 28132 novel splicing junctions.
sampling 30% (8097100) splicing reads. 180329 splicing junctions. 150180 known splicing junctions. 30149 novel splicing junctions.
sampling 35% (9446616) splicing reads. 183025 splicing junctions. 151197 known splicing junctions. 31828 novel splicing junctions.
sampling 40% (10796133) splicing reads. 185174 splicing junctions. 151924 known splicing junctions. 33250 novel splicing junctions.
sampling 45% (12145650) splicing reads. 186966 splicing junctions. 152532 known splicing junctions. 34434 novel splicing junctions.
sampling 50% (13495167) splicing reads. 188481 splicing junctions. 152999 known splicing junctions. 35482 novel splicing junctions.
sampling 55% (14844683) splicing reads. 189802 splicing junctions. 153397 known splicing junctions. 36405 novel splicing junctions.
sampling 60% (16194200) splicing reads. 190926 splicing junctions. 153730 known splicing junctions. 37196 novel splicing junctions.
sampling 65% (17543717) splicing reads. 191803 splicing junctions. 154010 known splicing junctions. 37793 novel splicing junctions.
sampling 70% (18893233) splicing reads. 192656 splicing junctions. 154263 known splicing junctions. 38393 novel splicing junctions.
sampling 75% (20242750) splicing reads. 193332 splicing junctions. 154450 known splicing junctions. 38882 novel splicing junctions.
sampling 80% (21592267) splicing reads. 193854 splicing junctions. 154592 known splicing junctions. 39262 novel splicing junctions.
sampling 85% (22941783) splicing reads. 194264 splicing junctions. 154699 known splicing junctions. 39565 novel splicing junctions.
sampling 90% (24291300) splicing reads. 194557 splicing junctions. 154775 known splicing junctions. 39782 novel splicing junctions.
sampling 95% (25640817) splicing reads. 194732 splicing junctions. 154822 known splicing junctions. 39910 novel splicing junctions.
sampling 100% (26990334) splicing reads. 194809 splicing junctions. 154843 known splicing junctions. 39966 novel splicing junctions.
null device 
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