[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00088_23H5VFLT4_s02.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (17442801) splicing reads. 254812 splicing junctions. 184051 known splicing junctions. 70761 novel splicing junctions.
sampling 10% (34885602) splicing reads. 308716 splicing junctions. 196740 known splicing junctions. 111976 novel splicing junctions.
sampling 15% (52328403) splicing reads. 346399 splicing junctions. 203749 known splicing junctions. 142650 novel splicing junctions.
sampling 20% (69771204) splicing reads. 375776 splicing junctions. 208328 known splicing junctions. 167448 novel splicing junctions.
sampling 25% (87214005) splicing reads. 399403 splicing junctions. 211568 known splicing junctions. 187835 novel splicing junctions.
sampling 30% (104656806) splicing reads. 418792 splicing junctions. 213986 known splicing junctions. 204806 novel splicing junctions.
sampling 35% (122099607) splicing reads. 435380 splicing junctions. 216032 known splicing junctions. 219348 novel splicing junctions.
sampling 40% (139542408) splicing reads. 449500 splicing junctions. 217645 known splicing junctions. 231855 novel splicing junctions.
sampling 45% (156985209) splicing reads. 461893 splicing junctions. 218989 known splicing junctions. 242904 novel splicing junctions.
sampling 50% (174428010) splicing reads. 472649 splicing junctions. 220134 known splicing junctions. 252515 novel splicing junctions.
sampling 55% (191870811) splicing reads. 481967 splicing junctions. 221022 known splicing junctions. 260945 novel splicing junctions.
sampling 60% (209313612) splicing reads. 490002 splicing junctions. 221804 known splicing junctions. 268198 novel splicing junctions.
sampling 65% (226756413) splicing reads. 496914 splicing junctions. 222500 known splicing junctions. 274414 novel splicing junctions.
sampling 70% (244199214) splicing reads. 503043 splicing junctions. 223100 known splicing junctions. 279943 novel splicing junctions.
sampling 75% (261642015) splicing reads. 508302 splicing junctions. 223557 known splicing junctions. 284745 novel splicing junctions.
sampling 80% (279084816) splicing reads. 512774 splicing junctions. 223957 known splicing junctions. 288817 novel splicing junctions.
sampling 85% (296527617) splicing reads. 516513 splicing junctions. 224288 known splicing junctions. 292225 novel splicing junctions.
sampling 90% (313970418) splicing reads. 519651 splicing junctions. 224585 known splicing junctions. 295066 novel splicing junctions.
sampling 95% (331413219) splicing reads. 522189 splicing junctions. 224850 known splicing junctions. 297339 novel splicing junctions.
sampling 100% (348856021) splicing reads. 523981 splicing junctions. 225013 known splicing junctions. 298968 novel splicing junctions.