Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e2/9036ca2723a28edf42d1a158f3bdbb/V4_0001_RNA_0005_23H5VFLT4_s37.starfusion.starfusion.fusion_predictions.tsv
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0a/2de37b681ace2e20a9e6fbe92bb26e/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0a/2de37b681ace2e20a9e6fbe92bb26e/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/stage-5b456d70-fab3-4fcc-916c-bc437f411fba/b2/04c094314651ca12aee230a027f8da/starfusion_filter_config.yaml
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/blacklist_breakpoint_database_2026-04-27.tsv
==> STAGING COMPLETE (6 inputs)

=== DEBUGGING INFORMATION ===
Current working directory: /tmp/nxf.KTOUdP8THV
Input files:
  - starfusion_tsv: V4_0001_RNA_0005_23H5VFLT4_s37.starfusion.starfusion.fusion_predictions.tsv
  - blacklist: blacklist_hg38_GRCh38_v2.4.0.tsv.gz
  - filter_config: starfusion_filter_config.yaml
  - aih_blacklist_breakpoints: blacklist_breakpoint_database_2026-04-27.tsv

=== PYTHON INFORMATION ===
/usr/local/bin/python
Python 3.11.14
/usr/local/bin/python3
Python 3.11.14

=== SCRIPT LOCATION ===
Checking for filter_starfusion_main.py:
/tmp/nxf.KTOUdP8THV/nextflow-bin/filter_starfusion_main.py
filter_starfusion_main.py not in current directory
bin/filter_starfusion_main.py not found
bin/ directory does not exist

=== ENVIRONMENT PATH ===
/tmp/nxf.KTOUdP8THV/nextflow-bin:/usr/local/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin

=== CURRENT DIRECTORY CONTENTS ===
total 64692
drwx------. 3 1000 root     4096 Jun  8 09:08 .
drwxrwxrwx. 4 root root       70 Jun  8 09:08 ..
-rw-r--r--. 1 1000 root        0 Jun  8 09:08 .command.err
-rw-r--r--. 1 1000 root      881 Jun  8 09:08 .command.out
-rw-r--r--. 1 1000 root    14577 Jun  8 09:08 .command.run
-rw-r--r--. 1 1000 root     3218 Jun  8 09:08 .command.sh
-rw-r--r--. 1 1000 root        0 Jun  8 09:08 .command.trace
-rw-r--r--. 1 1000 root   706954 Jun  8 09:08 V4_0001_RNA_0005_23H5VFLT4_s37.starfusion.starfusion.fusion_predictions.tsv
-rw-r--r--. 1 1000 root 10714998 Jun  8 09:08 blacklist_breakpoint_database_2026-04-27.tsv
-rw-r--r--. 1 1000 root 54779787 Jun  8 09:08 blacklist_hg38_GRCh38_v2.4.0.tsv.gz
drwxr-xr-x. 3 1000 root     4096 Jun  8 09:08 nextflow-bin
-rw-r--r--. 1 1000 root     4128 Jun  8 09:08 starfusion_filter_config.yaml

=== PROJECT DIRECTORY INFO ===
NXF_PROJECT_DIR: not set
baseDir: not set

=== Running filter script ===

================================================================================
STEP 1: LOADING STAR-FUSION PREDICTIONS
================================================================================
Loading STAR-Fusion predictions from: V4_0001_RNA_0005_23H5VFLT4_s37.starfusion.starfusion.fusion_predictions.tsv
  ✓ Loaded: 2170 fusions

================================================================================
STEP 2: CONVERTING TO BREAKPOINT_ID FORMAT
================================================================================
✓ Converted to breakpoint_ID format: 2170 valid, 0 invalid

================================================================================
STEP 3: FLAGGING WITH BLACKLIST
================================================================================
Loading blacklist (this may take a while)...
Loading blacklist from: blacklist_hg38_GRCh38_v2.4.0.tsv.gz
  Loaded 9381 single rules and 16721785 pair rules
  Indexed blacklist (bin_size=100000)
Flagging fusions with blacklist...
✓ Flagged 151 of 2170 fusions as blacklisted

================================================================================
STEP 3b: AIH CURATED BREAKPOINT BLACKLIST
================================================================================
Loading AIH blacklist table from: blacklist_breakpoint_database_2026-04-27.tsv
  Qualified breakpoint keys (orientations): 1378
  AIH curated blacklist: matched 71 fusion rows (breakpoint_ID in qualified set)

================================================================================
STEP 4: APPLYING YAML-BASED FILTER
================================================================================
✓ Filter initialized from: starfusion_filter_config.yaml
Applying filter criteria (adding CALL column)...
✓ Filter applied: 15 pass (CALL=1), 2155 fail (CALL=0)
  Total fusions: 2170 (all rows retained)
  Added FILTER column: PASS=15, FAIL=2155

✓ Verified: All 181 blacklisted fusions have CALL=0

================================================================================
STEP 5: GENERATING SUMMARY STATISTICS
================================================================================

================================================================================
FILTERING SUMMARY
================================================================================

Total fusions: 2170
  Pass (CALL=1): 15 (0.7%)
  Fail (CALL=0): 2155 (99.3%)

Blacklist Flagging:
  Total blacklisted: 181
  ✓ All 181 blacklisted fusions have CALL=0

Failure Reasons (top 10):
  Read count too low: 1 < 8: 1183
  Excluded SpliceType: INCL_NON_REF_SPLICE: 709
  Blacklisted: 110
  Read count too low: 2 < 8: 44
  Blacklisted; aih_curated_blacklist score(3): 41
  aih_curated_blacklist score(3): 28
  Excluded annotation pattern: HGNC_GENEFAM: 22
  Read count too low: 5 < 8: 4
  Excluded annotation pattern: DGD_PARALOGS: 4
  Read count too low: 6 < 8: 2

Breakdown by SpliceType:
  INCL_NON_REF_SPLICE: 1 pass, 1068 fail (total: 1069)
  ONLY_REF_SPLICE: 14 pass, 1087 fail (total: 1101)

Read Count Statistics:
  Fusions with < 3 total reads: 2073
  Of these, 2073 have CALL=0

================================================================================
STEP 6: EXPORTING RESULTS
================================================================================
✓ Results exported to: V4_0001_RNA_0005_23H5VFLT4_s37.starfusion.starfusion.fusion_predictions.filtered.tsv
  Total fusions: 2170
  CALL=1 (pass): 15
  CALL=0 (fail): 2155
✓ Passing fusions (CALL=1) exported to: V4_0001_RNA_0005_23H5VFLT4_s37.starfusion.starfusion.fusion_predictions.filtered_pass_only.tsv
✓ Summary exported to: V4_0001_RNA_0005_23H5VFLT4_s37.starfusion.starfusion.fusion_predictions.filter_summary.txt

================================================================================
✓ FILTERING COMPLETE
================================================================================