Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2f/9055d4b0da3d2fb2a1c6cf662c7616/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/15/03ea68d13b8030c917b2ccb1ae1672/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/15/03ea68d13b8030c917b2ccb1ae1672/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/38/7cd2061c1c16e215b06cb192370845/tih_rna_sample_00115_23H5VFLT4_s35.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f2/da47d93bf4d914f2e2abc11162b7d0/tih_rna_sample_00115_23H5VFLT4_s35.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00115_23H5VFLT4_s35.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (10109850) splicing reads. 229221 splicing junctions. 174040 known splicing junctions. 55181 novel splicing junctions.
sampling 10% (20219701) splicing reads. 270573 splicing junctions. 185325 known splicing junctions. 85248 novel splicing junctions.
sampling 15% (30329551) splicing reads. 296217 splicing junctions. 190792 known splicing junctions. 105425 novel splicing junctions.
sampling 20% (40439402) splicing reads. 314493 splicing junctions. 194231 known splicing junctions. 120262 novel splicing junctions.
sampling 25% (50549252) splicing reads. 328332 splicing junctions. 196546 known splicing junctions. 131786 novel splicing junctions.
sampling 30% (60659103) splicing reads. 339249 splicing junctions. 198331 known splicing junctions. 140918 novel splicing junctions.
sampling 35% (70768953) splicing reads. 348084 splicing junctions. 199701 known splicing junctions. 148383 novel splicing junctions.
sampling 40% (80878804) splicing reads. 355054 splicing junctions. 200703 known splicing junctions. 154351 novel splicing junctions.
sampling 45% (90988654) splicing reads. 361219 splicing junctions. 201528 known splicing junctions. 159691 novel splicing junctions.
sampling 50% (101098505) splicing reads. 366269 splicing junctions. 202231 known splicing junctions. 164038 novel splicing junctions.
sampling 55% (111208355) splicing reads. 370509 splicing junctions. 202808 known splicing junctions. 167701 novel splicing junctions.
sampling 60% (121318206) splicing reads. 373975 splicing junctions. 203271 known splicing junctions. 170704 novel splicing junctions.
sampling 65% (131428056) splicing reads. 377063 splicing junctions. 203670 known splicing junctions. 173393 novel splicing junctions.
sampling 70% (141537907) splicing reads. 379507 splicing junctions. 203989 known splicing junctions. 175518 novel splicing junctions.
sampling 75% (151647757) splicing reads. 381685 splicing junctions. 204251 known splicing junctions. 177434 novel splicing junctions.
sampling 80% (161757608) splicing reads. 383345 splicing junctions. 204471 known splicing junctions. 178874 novel splicing junctions.
sampling 85% (171867458) splicing reads. 384673 splicing junctions. 204639 known splicing junctions. 180034 novel splicing junctions.
sampling 90% (181977309) splicing reads. 385605 splicing junctions. 204748 known splicing junctions. 180857 novel splicing junctions.
sampling 95% (192087159) splicing reads. 386247 splicing junctions. 204825 known splicing junctions. 181422 novel splicing junctions.
sampling 100% (202197010) splicing reads. 386598 splicing junctions. 204860 known splicing junctions. 181738 novel splicing junctions.
null device 
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