Done. -parsing GTF file: /tmp/nxf.R3SmTO1c36/tih_rna_sample_00116_23H5VFLT4_s36.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.R3SmTO1c36/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.R3SmTO1c36/tih_rna_sample_00116_23H5VFLT4_s36_1.fastp.fastq.gz /tmp/nxf.R3SmTO1c36/tih_rna_sample_00116_23H5VFLT4_s36_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.R3SmTO1c36/fi_workdir/tih_rna_sample_00116_23H5VFLT4_s36.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.R3SmTO1c36/fi_workdir/tih_rna_sample_00116_23H5VFLT4_s36.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam tih_rna_sample_00116_23H5VFLT4_s36.star.sortedByCoord.out.bam * Running CMD: samtools index tih_rna_sample_00116_23H5VFLT4_s36.star.sortedByCoord.out.bam 10:37:05 : INFO : Done. -parsing GTF file: /tmp/nxf.R3SmTO1c36/fi_workdir/tih_rna_sample_00116_23H5VFLT4_s36.gtf -parsing /tmp/nxf.R3SmTO1c36/fi_workdir/tih_rna_sample_00116_23H5VFLT4_s36.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] [160000] [170000] [180000] [190000] [200000] [210000] [220000] [230000] [240000] [250000] [260000] [270000] -done parsing /tmp/nxf.R3SmTO1c36/fi_workdir/tih_rna_sample_00116_23H5VFLT4_s36.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'num_hits: 5 != num_counted_on_fusion_contigs 2 ' => 14, 'num_hits: 5 != num_counted_on_fusion_contigs 4 ' => 4, 'num_hits: 3 != num_counted_on_fusion_contigs 2 ' => 8, 'num_hits: 5 != num_counted_on_fusion_contigs 3 ' => 24, 'per_id < 96' => 1905, 'seq-similar region overlap' => 193, ' ** passed ** ' => 2310, 'num genes matched < 2' => 2, 'small anchor length' => 8, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 18, 'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 6, 'excessive soft clipping' => 3470 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.R3SmTO1c36/fi_workdir/tih_rna_sample_00116_23H5VFLT4_s36.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] -fusion SPANNING read extraction for scaff: AC013268.1--MALL [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] -fusion SPANNING read extraction for scaff: AC092807.3--DDAH1 [1000] [2000] [3000] -fusion SPANNING read extraction for scaff: LINC00937--AC083906.5 [1000] [2000] [3000] -fusion SPANNING read extraction for scaff: LINC00937--AC083906.2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] -fusion SPANNING read extraction for scaff: ZNF587--CLASRP -filtered reads reasons: $VAR1 = { 'seq similar region alignment' => 18934, 'lacks exon overlap' => 5272 }; EM: Starting log likelihood: -1656.805582 EM: Round [1] log likelihood: -1656.287130 EM: Round [2] log likelihood: -1655.821445 EM: Round [3] log likelihood: -1655.401864 EM: Round [4] log likelihood: -1655.022796 EM: Round [5] log likelihood: -1654.679514 EM: Round [6] log likelihood: -1654.367983 EM: Round [7] log likelihood: -1654.084735 EM: Round [8] log likelihood: -1653.826771 EM: Round [9] log likelihood: -1653.591481 EM: Round [10] log likelihood: -1653.376585 EM: Round [11] log likelihood: -1653.180076 EM: Round [12] log likelihood: -1653.000184 EM: Round [13] log likelihood: -1652.835342 EM: Round [14] log likelihood: -1652.684154 EM: Round [15] log likelihood: -1652.545377 EM: Round [16] log likelihood: -1652.417897 EM: Round [17] log likelihood: -1652.300716 EM: Round [18] log likelihood: -1652.192936 EM: Round [19] log likelihood: -1652.093746 EM: Round [20] log likelihood: -1652.002415 EM: Round [21] log likelihood: -1651.918280 EM: Round [22] log likelihood: -1651.840742 EM: Round [23] log likelihood: -1651.769255 EM: Round [24] log likelihood: -1651.703322 EM: Round [25] log likelihood: -1651.642492 EM: Round [26] log likelihood: -1651.586353 EM: Round [27] log likelihood: -1651.534528 EM: Round [28] log likelihood: -1651.486674 EM: Round [29] log likelihood: -1651.442475 EM: Round [30] log likelihood: -1651.401644 EM: Round [31] log likelihood: -1651.363917 EM: Round [32] log likelihood: -1651.329050 EM: Round [33] log likelihood: -1651.296822 EM: Round [34] log likelihood: -1651.267028 EM: Round [35] log likelihood: -1651.239480 EM: Round [36] log likelihood: -1651.214005 EM: Round [37] log likelihood: -1651.190445 EM: Round [38] log likelihood: -1651.168653 EM: Round [39] log likelihood: -1651.148494 EM: Round [40] log likelihood: -1651.129844 EM: Round [41] log likelihood: -1651.112589 EM: Round [42] log likelihood: -1651.096622 EM: Round [43] log likelihood: -1651.081847 EM: Round [44] log likelihood: -1651.068174 EM: Round [45] log likelihood: -1651.055519 EM: Round [46] log likelihood: -1651.043806 EM: Round [47] log likelihood: -1651.032965 EM: Round [48] log likelihood: -1651.022929 EM: Round [49] log likelihood: -1651.013639 EM: Round [50] log likelihood: -1651.005039 EM: Round [51] log likelihood: -1650.997077 EM: Round [52] log likelihood: -1650.989705 EM: Round [53] log likelihood: -1650.982880 EM: Round [54] log likelihood: -1650.976561 EM: Round [55] log likelihood: -1650.970710 EM: Round [56] log likelihood: -1650.965292 EM: Round [57] log likelihood: -1650.960276 EM: Round [58] log likelihood: -1650.955631 EM: Round [59] log likelihood: -1650.951329 EM: Round [60] log likelihood: -1650.947346 EM: Round [61] log likelihood: -1650.943657 EM: Round [62] log likelihood: -1650.940241 EM: Round [63] log likelihood: -1650.937078 EM: Round [64] log likelihood: -1650.934148 EM: Round [65] log likelihood: -1650.931435 EM: Round [66] log likelihood: -1650.928922 EM: Round [67] log likelihood: -1650.926595 EM: Round [68] log likelihood: -1650.924440 EM: Round [69] log likelihood: -1650.922444 EM: Round [70] log likelihood: -1650.920595 EM: Round [71] log likelihood: -1650.918883 EM: Round [72] log likelihood: -1650.917297 EM: Round [73] log likelihood: -1650.915828 EM: Round [74] log likelihood: -1650.914468 EM: Round [75] log likelihood: -1650.913208 EM: Round [76] log likelihood: -1650.912041 EM: Round [77] log likelihood: -1650.910960 EM: Round [78] log likelihood: -1650.909959 EM: Round [79] log likelihood: -1650.909032 EM: Round [80] log likelihood: -1650.908173 EM: Round [81] log likelihood: -1650.907378 EM: Round [82] log likelihood: -1650.906641 EM: Round [83] log likelihood: -1650.905959 EM: Round [84] log likelihood: -1650.905327 EM: Round [85] log likelihood: -1650.904742 EM: Round [86] log likelihood: -1650.904199 EM: Round [87] log likelihood: -1650.903697 EM: Round [88] log likelihood: -1650.903232 EM: Round [89] log likelihood: -1650.902801 EM: Round [90] log likelihood: -1650.902402 EM: Round [91] log likelihood: -1650.902032 EM: Round [92] log likelihood: -1650.901690 EM: Round [93] log likelihood: -1650.901373 EM: Round [94] log likelihood: -1650.901079 EM: Round [95] log likelihood: -1650.900807 EM: Round [96] log likelihood: -1650.900555 EM: Round [97] log likelihood: -1650.900322 EM: Round [98] log likelihood: -1650.900105 EM: Round [99] log likelihood: -1650.899905 EM: Round [100] log likelihood: -1650.899720 EM: Round [101] log likelihood: -1650.899548 EM: Round [102] log likelihood: -1650.899389 EM: Round [103] log likelihood: -1650.899241 EM: Round [104] log likelihood: -1650.899105 EM: Round [105] log likelihood: -1650.898978 EM: Round [106] log likelihood: -1650.898861 EM: Round [107] log likelihood: -1650.898752 EM: Round [108] log likelihood: -1650.898652 EM: Round [109] log likelihood: -1650.898559 EM: Stopping iterations at round 109 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.R3SmTO1c36/tih_rna_sample_00116_23H5VFLT4_s36.consolidated.bam into /tmp/nxf.R3SmTO1c36/tih_rna_sample_00116_23H5VFLT4_s36.consolidated.bam.read_coords CMD: touch /tmp/nxf.R3SmTO1c36/tih_rna_sample_00116_23H5VFLT4_s36.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.R3SmTO1c36/tih_rna_sample_00116_23H5VFLT4_s36.consolidated.bam.read_coords > /tmp/nxf.R3SmTO1c36/tih_rna_sample_00116_23H5VFLT4_s36.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.R3SmTO1c36/tih_rna_sample_00116_23H5VFLT4_s36.consolidated.bam.frag_coords > /tmp/nxf.R3SmTO1c36/tih_rna_sample_00116_23H5VFLT4_s36.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.R3SmTO1c36/fi_workdir/tih_rna_sample_00116_23H5VFLT4_s36.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.R3SmTO1c36/fi_workdir/tih_rna_sample_00116_23H5VFLT4_s36.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.R3SmTO1c36/fi_workdir/tih_rna_sample_00116_23H5VFLT4_s36.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.R3SmTO1c36/tih_rna_sample_00116_23H5VFLT4_s36.post_blast_and_promiscuity_filter -done, see /tmp/nxf.R3SmTO1c36/tih_rna_sample_00116_23H5VFLT4_s36.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -1577.000478 EM: Round [1] log likelihood: -1577.000478 EM: Stopping iterations at round 1 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.R3SmTO1c36/tih_rna_sample_00116_23H5VFLT4_s36_1.fastp.fastq.gz: 296460757 Warning - not locating file: /tmp/nxf.R3SmTO1c36/tih_rna_sample_00116_23H5VFLT4_s36.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000186148.13] WARNING, no entry stored in dbm for [ENSG00000186148.13] WARNING, no entry stored in dbm for [ENSG00000186148.13] WARNING, no entry stored in dbm for [ENSG00000186148.13] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] ls: cannot access 'IGV_inputs': No such file or directory