[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00104_23H5VFLT4_s20.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (7484555) splicing reads. 206611 splicing junctions. 164588 known splicing junctions. 42023 novel splicing junctions.
sampling 10% (14969111) splicing reads. 234933 splicing junctions. 173983 known splicing junctions. 60950 novel splicing junctions.
sampling 15% (22453667) splicing reads. 251281 splicing junctions. 178207 known splicing junctions. 73074 novel splicing junctions.
sampling 20% (29938222) splicing reads. 262615 splicing junctions. 180668 known splicing junctions. 81947 novel splicing junctions.
sampling 25% (37422778) splicing reads. 271068 splicing junctions. 182351 known splicing junctions. 88717 novel splicing junctions.
sampling 30% (44907334) splicing reads. 277877 splicing junctions. 183639 known splicing junctions. 94238 novel splicing junctions.
sampling 35% (52391889) splicing reads. 283357 splicing junctions. 184600 known splicing junctions. 98757 novel splicing junctions.
sampling 40% (59876445) splicing reads. 287922 splicing junctions. 185271 known splicing junctions. 102651 novel splicing junctions.
sampling 45% (67361001) splicing reads. 291830 splicing junctions. 185833 known splicing junctions. 105997 novel splicing junctions.
sampling 50% (74845557) splicing reads. 295116 splicing junctions. 186285 known splicing junctions. 108831 novel splicing junctions.
sampling 55% (82330112) splicing reads. 297894 splicing junctions. 186680 known splicing junctions. 111214 novel splicing junctions.
sampling 60% (89814668) splicing reads. 300354 splicing junctions. 187010 known splicing junctions. 113344 novel splicing junctions.
sampling 65% (97299224) splicing reads. 302368 splicing junctions. 187275 known splicing junctions. 115093 novel splicing junctions.
sampling 70% (104783779) splicing reads. 304090 splicing junctions. 187530 known splicing junctions. 116560 novel splicing junctions.
sampling 75% (112268335) splicing reads. 305589 splicing junctions. 187701 known splicing junctions. 117888 novel splicing junctions.
sampling 80% (119752891) splicing reads. 306767 splicing junctions. 187833 known splicing junctions. 118934 novel splicing junctions.
sampling 85% (127237446) splicing reads. 307641 splicing junctions. 187944 known splicing junctions. 119697 novel splicing junctions.
sampling 90% (134722002) splicing reads. 308332 splicing junctions. 188030 known splicing junctions. 120302 novel splicing junctions.
sampling 95% (142206558) splicing reads. 308752 splicing junctions. 188091 known splicing junctions. 120661 novel splicing junctions.
sampling 100% (149691114) splicing reads. 308961 splicing junctions. 188120 known splicing junctions. 120841 novel splicing junctions.